Ajayi, T. O.Srivedavyasasri, R.Nyong, E. E.Odeniyi, M. A.Moody, J. O.Ross, S. A.2026-02-1220190039-128X1878-5867ui_art_ajayi_two_2019Steroidshttps://repository.ui.edu.ng/handle/123456789/12134The crude methanol extract of Sphenocentrum jollyanum root exhibited 98% and 80% antimicrobial activity against Aspergillus fumigatus Pinh and Vancomycin resistant enterococcus (VRE) at a concentration of 200 μg/mL, with ICso 11.45 and 12.95 μg/mL, respectively. The ethyl acetate fraction of methanol extract showed in-vitro antimicrobial activity against A. fumigatus Pinh at 83% with ICso of <8 μg/mL. The phytochemical investigation of ethyl acetate fraction yielded six compounds, which were identified by their NMR, IR and MS spectral analyses as two new phytoecdysteroidal glycosides Sphenocentroside A (1), and NUSART Sphenocentroside B (2), and four known phytoecdysteroids: polypodoaurein (3), polypodine B (4), ecdysterone (5), and 20, 26-dihydroxyecdysone (6).enSphenocentrum jollyanumPhytoecdysteroidsAntimicrobial activityAspergillus fumigatusVancomycin resistant enterococcusArticle