Browsing by Author "Adeniji, J. A"

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    Characterization of hepatitis delta virus strains spreading in Abuja, Nigeria
    (Wiley Periodicals, Inc.,, 2019) Ifeorah, I. M.; Faleye, T. O. C.; Bakarey, A. S.; Adewumi, O. M.; Gerber, A.; Le Gal, F.; Adeniji, J. A; Gordien, E.; Onyemelukwe, N. F.
    Hepatitis delta virus (HDV) is responsible for the most severe form of liver disease in humans. So far, eight genotypes (HDV‐1 to ‐8) have been individualized worldwide. Little is known about HDV strains that spread in Nigeria. HDV genotyping was performed in 15 anti–HDV positive samples from a cohort of 306 hepatitis B virus (HBV)‐infected patients in Abuja (Nigeria). Phylogenetic analyses revealed 90% were HDV‐1, two among them clustering with European/Asian HDV‐1, the remaining one being HDV‐6. It was also found that two members of a couple superinfected with the same HDV strain, were enveloped by two different HBV strains of genotype E.
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    Genome sequences of novel members of previously described DNA and RNA virus families, isolated from feces of a drill monkey in Nigeria
    (American Society for Microbiology (ASM), 2020) George,U.; Simsek, C.; Faleye, T. O. C.; Arowolo,O. A.; Oragwa, A.; Adewumi,O. M.; Matthijnssens, J.; Adeniji, J. A; Adeniji, J. A
    The genomes of seven novel members of previously described DNA and RNA virus families are described here. These viruses were recovered using a viral metagenomic approach from the stool of a drill monkey (Mandrillus leucophaeus) housed in a sanctuary in Cross River State, Nigeria.
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    Prevalence of hepatitis b virus (HBV) basal core promoter/precore region molecular variants among HIV/HBV co-infected and HBV mono-infected patients in Ile-Ife, Nigeria
    (Medwin Publishers, 2021) Osasona, O. G.; Boudarene, L.; Fasoro, O. A.; George, U. E.; Oguzie, J.; Ariyo, O. E.; Olumade, T. J.; Adeniyi, A.; Adewumi, O. M.; Adeniji, J. A
    Introduction: Evolution of phenotypic diversity among viruses occurs as an escape mechanism against host immune pressure or drug selective pressure. Among HIV/HBV co-infected individuals, various HBV basal core promoter (BCP)/precore (PC) region molecular mutants had been reported with associated phenotypic defect in HBeAg production. The emergence of HBeAg negative variants of HBV in HIV co-infected individuals have profound implication on the diagnosis, management and prognosis of this subset of individuals. This includes delayed clearance of HBV, early development of adverse hepatic events such as liver cirrhosis and hepatocellular carcinoma. Currently, little is known about HBV BCP/PC region genomic heterogeneity in HIV/HBV co-infected patients in Nigeria. Therefore, this study was focused on investigating evidence of precore/core region genomic variability among HIV/HBV co-infected patients in Nigeria. Materials and Methods: A total of 40 patients (20 HIV/HBV co-infected and 20 HBV mono-infected samples) were enrolled into the study and subsequently tested for HBsAg, HBeAg and HBeAb using specific Enzyme-Linked Immunosorbent Assay (ELISA). The BCP/PC genome regions (nucleotides 1653-1959) were amplified using a nested PCR assay and then subjected to BCP/PC mutational analysis in genome sites affecting HBeAg expression especially at the BCP transcriptional and PC Translational stop codon sites. Results: Overall, 5 (83.3%) of the six exploitable sequences after analysis showed various BCP/PC mutations. Only 1(16.6%) sequence from an HIV/HBV co-infected patient had the BCP transcriptional (double mutation; A1762T/G1764A) mutant. Analysis of the PC translational stop codon showed 4 (66.6%) having the G1896A mutants while 33.3% (2) had G1899A mutants. Conclusion: This study has broadened the available evidence of BCP/PC region molecular mutants among HIV/HBV coinfected patients in Nigeria and assessed the difference of mutation prevalence in comparison with HBV mono-infected cohort.