Browsing by Author "Adeniji, J.A."

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Direct Detection and Identification of Enteroviruses from Faeces of Healthy Nigerian Children Using a Cell-Culture Independent RT-Seminested PCR Assay
(Hindawi Publishing Corporation, 2016) Faleye, T. O. C.; Adewumi, M. O.; Coker, B. A.; Nudamajo, F. Y.; Adeniji, J.A.
Recently, a cell-culture independent protocol for detection of enteroviruses fromclinical specimen was recommended by theWHO for surveillance alongside the previously established protocols. Here, we investigated whether this new protocol will show the same enterovirus diversity landscape as the established cell-culture dependent protocols. Faecal samples were collected from sixty apparently healthy children in Ibadan,Nigeria. Samples were resuspended in phosphate buffered saline, RNAwas extracted, and the VP1 gene was amplified using WHO recommended RT-snPCR protocol. Amplicons were sequenced and sequences subjected to phylogenetic analysis. Fifteen (25%) of the 60 samples yielded the expected band size. Of the 15 amplicons sequenced, 12 were exploitable. The remaining 3 had electropherograms with multiple peaks and were unexploitable. Eleven of the 12 exploitable sequences were identified as Coxsackievirus A1 (CVA1), CVA3, CVA4, CVA8, CVA20, echovirus 32 (E32), enterovirus 71 (EV71), EVB80, and EVC99. Subsequently, the last exploitable sequence was identified as enterobacteriophage baseplate gene by nucleotide BLAST. Theresults of this study document the first description of molecular sequence data on CVA1, CVA8, and E32 strains present in Nigeria. The result further showed that species A enteroviruses were more commonly detected in the region when cell-culture bias is bypassed.
Draft Genome Sequence of Mycoplasma arginini Strain NGR_2017
(Merican Society for Microbiology (ASM), 2018-06-28) Adeniji, J.A.; Faleye, T. O. C.; Adewu , M. O.; Olayinka O. A.; Donbraye, E.; Oluremi, B.; George,a U. E.; Arowolo, O. A.; Omoruy, E. C.; Ifeorah, M. I.; Akande, A.
We present the draft genome of Mycoplasma arginini strain NGR_2017. This strain was recovered in Nigeria from cell culture in 2017. The assembly contains 620,555 bp in 12 contigs. It contains 561 coding sequences, 34 RNAs (29 tRNAs, 4rRNAs, and 1 transfer-messenger RNA [tmRNA]), and a_26-kb integrative and conjugative element.
Evaluation of CD4 T lymphocyte cell Levels among Hepatitis B, C and E Viruses negative individuals in Ibadan, Southwestern Nigeria
(SCIENCEDOMAIN International, 2017) Adewumi, M. O .; Omoruyi, E. C.; Ifeorah, I. M.; Bakarey, A. S.; Ogunwale, A. O.; Akere, A.; Faleye, T. O. C.; Adeniji, J.A.
Aim: The CD4 T lymphocytes play a key role in achieving a regulated effective immune response to foreign antigens. It is also a valuable parameter for assessing HIV disease progression. However, variations in CD4 T lymphocyte values due to diverse factors have been reported. Here we evaluated CD4 T lymphocytes among community dwellers who tested negative for hepatitis B, hepatitis C and hepatitis E viruses and compared the results with the National Reference Values (NRVs).Study Design: A cross-sectional study was conducted. Participants were enrolled using a convenient sampling technique and their socio-demographic characteristics were captured by administration of semi-structured questionnaires. Place and Duration of Study: This study was conducted among residents of Ibadan metropolis, Southwestern Nigeria. Participants were enrolled between July and September, 2013 at the University College Hospital, Ibadan. Methodology: Four hundred consenting participants who fulfilled the criteria for enrolment were evaluated for CD4 T lymphocyte counts. Results: Estimated mean CD4 T lymphocyte count of 1,183 (CD4 Range: 328-2680) cells/μl of blood was recorded for the participants. Four (1.0%), 151 (37.8%), 157 (39.2%), 74 (18.5), and 14(3.5) of the participants had CD4 T lymphocyte count ranged 352-500, 501-1,000, 1,001-1500, 1501-2,000, and >2,000 cells/μl of blood, respectively. Differences in the estimated mean CD4 count between different age groups varied significantly (P=0.010).Conclusion: In this study, significantly higher CD4 T lymphocyte values were observed among the study population in comparison to the NRVs, and consequently we advise careful interpretation and use of extrapolated CD4 T lymphocyte values in the management of persons with diverse geographical background or health conditions.
Genome Sequences of Two Dual-Serotype-Specific Echovirus 20 Strains from Nigeria
(American Society for Microbiology (ASM), 2020) Faleye, T. O. C.; Adewumi, O. M.; Klapsa, D.; Majumdar, M.; Martin, J.; Adeniji, J.A.
Here, we describe nearly complete genome sequences (7,361 nucleotides [nt] and 6,893 nt) of two echovirus 20 (E20) isolates from Nigeria that were simultaneously typed as CVB and E20 (dual serotype) by neutralization assay. Both include two overlapping open reading frames (ORFs) of 67 and 2,183 amino acids that encoded a recently described gut infection-facilitating protein and the classic enterovirus proteins, respectively.
Near-complete genome sequence of an enterovirus species fisolate recovered from sewage in Nigeria
(American Society for Microbiology (ASM), 2021) Faleye, T. O. C.; Ifeorah, M. I.; Olayinka, O. A.; Oluremi, B.; George, U. E.; Arowolo, O. A.; Omoruyi, E. C.; Donbraye, E.; Oyero, A. O.; Adewumi, O. M.; Adeniji, J.A.
Here, we describe the near-complete genome of an enterovirus F (EV-F) isolate from Nigeria. The obtained sequence was 7,378 nucleotides (nt) long and encodes 2 open reading frames (ORFs), an upstream ORF (uORF; 56 amino acids [aa])and a polyprotein ORF (ppORF; 2,167 aa). Both ORFs overlap but are in different reading frames, with the uORF in a _1 reading frame relative to the ppORF.