Browsing by Author "Adetosoye, A. I."

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Field and experimental investigations of an outbreak of African swine in Nigeria
(2005) Otesile, E. B.; Ajuwape, A. T. P.; Odemuyiwa, S. O.; Akpavie, S. O.; Olaifa, A. K.; Odaibo, G. N.; Olaleye, O. D.; Adetosoye, A. I.
An African swine fever (ASF), characterized by a mortality reaching 50 to 100 p. 100 in different herds were diagnosed with high domestic pigs loose in Delta State in Nigeria in August 1998. The etiologic confirmation of the PPP was obtained by virus isolation, PCR and sequencing of 280 base pairs a segment of the gene encoding the major protein (VP72) capsid. The experimental infection of animals with infected blood caused fevers, with the highest peaks two to four days after infection, death followed five to six days after infection. Post-mortem examinations revealed very extensive bleeding and the appearance of congested and edematous tissue. The lymph nodes, spleen, liver and kidneys exhibited pronounced random focal necrosis and loss of cells in the follicles of the spleen and lymphoid tissue. Pigs have also revealed acute orchitis with massive neutrophilic infiltrates and macrophages in intertubular connective tissues. of meningitis and focal hemorrhages were observed in the brain and spinal cord. It seems that the home came from the spread east of the PPA, from Benin, neighboring state where the PPP had declared the previous year (1997)
Polymerase chain reaction assay of ureaplasma strains isolated from high vaginal swabs of women in Ibadan, Nigeria
(College of Medicine, University of Ibadan, Ibadan, 2008) Agbakoba, N. R.; Adetosoye, A. I.; Adesina, O. A.; Adewole, I. F.
Human ureaplasma previously had one species known as Ureaplasma urealyticum but was recently separated into 2 species, U. urealyticum and U. parvum. This study was carried out to separate the ureaplasma strains isolated from women attending a tertiary-care hospital in Nigeria. Thirty (30) Ureaplasma strains isolated from the vaginal tracts of 13 pregnant and 17 non-pregnant women were assayed. The polymerase chain reaction (PCR) technique was employed using two primer pairs: UMS-170/UMA-263 specific for U. urealyticum and UMS-57/UMA-222 specific for U. parvum. The positivity bands of the primer pairs were 476 bp and 326 bp for U. urealyticum and U. parvum respectively. All isolates were found to be U. urealyticumn (100%). Eleven (84.6%) of the 13 U. urealyticum from pregnant women were from asymptomatic women while from the non-pregnant women; 6 (35.3%) were from women with complaint of infertility problems; 5 (29.4%) from those who complained of vaginal discharge, one (5.9%) was asymptomatic while the remaining 5 (29.4%) had various other complaints. U. urealyticum is thus the prevalent species of Ureaplasma among pregnant and non-pregnant women in the study population and this to the best of our knowledge is a pioneer study to speciate human ureaplasmas in this country.