Browsing by Author "Aiyelaagbe, O. O."

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    Anti-measles virus activity of 4-hydroxy-3-methoxy benzaldehyde (Vanillin) isolated from xylopia aethiopica (Dunal) A. rich
    (Elsevier B.V., 2023) Oluremi, B. B.; Osamudiamen, P. M.; Adeniji, J. A.; Aiyelaagbe, O. O.
    Xylopia aethiopica is a plant used ethnomedicinally for the treatment of several infections in Nigeria. This study was carried out to isolate the active compound(s) in Xylopia aethiopica and evaluate their anti-measles virus activity. The Dichloromethane (DCM) fraction of Xylopia aethiopica was fractionated using chromatographic techniques, which led to the isolation of a compound characterized using spectroscopic techniques, FT-IR, 1D and 2D NMR in addition to in vitro anti-measles evaluation in adsorption and post-infection inhibition assays. The isolated compound characterized as 4-Hydroxy-3-methoxy benzaldehyde (Vanillin) was active on measles virus and has minimum nontoxic dose of 10 μg with cytotoxic and inhibitory activity of CC 50 = 84.18 μg/mL, IC 50 = 0.71 μg/mL and selectivity index (SI) = 118.56 (r 2 = 0.979) and interfered with viral attachment and fusion. This is the first report of the isolation of Vanillin from Xylopia aethiopica leaf and its high antiviral activity shows it could be developed further into a promising antiviral lead compound.
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    Trans -resveratrol, piceatannol and gallic acid: potent polyphenols isolated from Mezoneuron benthamianum effective as anticaries, antioxidant and cytotoxic agents
    (Elsevier B.V. on behalf of African Institute of Mathematical Sciences, 2020) Osamudiamen, P. M.; Oluremi, B. B.; Oderinlo, O. O.; Aiyelaagbe, O. O.
    Phytochemical investigation of the ethyl acetate extract of the root of Mezoneuron ben- thamianum , a shrub commonly used as chewing stick in the southwest of Nigeria, led to the isolation of three compounds characterised as trans -resveratrol, piceatannol and gallic acid by the use of spectroscopic techniques. The anticaries activity of the polyphenols was evaluated against four oral bacteria pathogens ( Streptococcus mutans, Pseudomonas aerugi- nosa, Staphylococcus aureus and Escherichia coli), isolated from clinical samples presented by patients having dental caries, while their antioxidant and cytotoxic activities were deter- mined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and brine shrimp ( artemia salina nau- plii) bioassay techniques. The results showed that the isolated compounds exhibited very strong anticaries activity with minimum inhibitory concentration (MIC) ranging from 25 to 300 μg/ml, antioxidant activity with IC 50 of 35.81, 30.35 and 11.73 μg/ml, when com- pared with ascorbic acid (IC 50 = 38.20 μg/ml) and cytotoxic activity with LC 50 of 7.15, 99.99 and 98.71 μg/ml for resveratrol, piceatannol and gallic acid respectively The isolated compounds demonstrated very strong anticaries, antioxidant and cytotoxic activities. This study further justifies the ethno-medicinal use of M. benthamianum as an effective tool in maintaining oral hygiene.