Browsing by Author "Ajani, O. S."

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Effects of ageratum conyzoides on semen characteristics and sperm morphology in rats exposed to sodium arsenite
(Biomedical Communications Group, Ibadan, 2016-01) Ola-Davies, O.; Ajani, O. S.
Arsenite is a major environmental toxicant that is well known to cause reproductive injury. The sperm protective potential of Ageratum conyzoides Linn in arsenic-treated rats was carried out in this study taking advantage of the antioxidant constituents and its androgenic activities. Twenty-four male albino rats aged 16 weeks, weighing 225 to 228g were used. They were grouped into 4(A-Da) with each group containing 6 rats. Group A was orally treated with 100mg/kg ethanol leaf extract of Ageratum conyzoides L., daily for 14 days, group B (single oral dose of sodium arsenite 2.5 mg/kg body weight), C (Ageratum conyzoides extract daily for 14 days and sodium arsenite (SA) given on the 14th day) and group D (Propylene glycol as negative control). It was observed that group B had a lower (p<0.05) percentage motility (26.7±6.67%) when compared across the groups while group A had a significantly higher (p<0.05) mean value (63.3±3.33%). The sperm motility of rats in group D was significantly higher (p<0.05) than groups B and C. This implies that A. conyzoides extract had no adverse effect on the sperm motility of the rats and also ameliorates the adverse effect of arsenite on sperm motility. The mean value obtained for sperm liveability, semen volume and Sperm concentration followed a similar pattern although, the differences were not significant (p>0.05) for semen volume and the Sperm concentration of rats across the groups. The total sperm abnormality obtained across the groups ranges between 10.44 and 14.27% with group B treated with sodium arsenite (SA) having the highest value when compared with groups A and D, although, the differences were not significant (P>0.05). The study concluded that ethanol leaf extract of Ageratum conyzoides has no negative effect on sperm motility, liveability characteristics and morphology and also protected spermatozoa against arsenic reproductive toxicity in Wistar strain albino rats.
Spermatozoa morphology and characteristics of spondias mombin L. (anacardiaceae) protected male Wistar rats exposed to sodium arsenite
(Academic Journals, 2014-02) Ola-Davies, O.; Ajani, O. S.; Oyeyemi, M. O.
The sperm protective potential of Spondias mombin L in arsenic-treated rats was carried out. Thirty-five male albino rats (225 to 228 g) were used and grouped into 7 (A to G), each group containing 5 rats. Group A was treated with 0.1 ml dimethyl sulphoxide (DMSO), B (0.1 ml of distilled water), C (sodium arsenite (SA-2.5 mg/kg body weight), D (ethyl acetate fraction), E (ethyl acetate fraction for 7 days and sodium arsenite the 7th day), F (methanolic fraction for 7 days and sodium arsenite the 7th day) and G (methanolic fraction for 7 days). It was observed that groups G and E had the lowest percentage motility 25.00±15.00 and 38.00±13.90%, respectively which were significantly lower (P<0.05) than groups A and B (89.00±2.45 and 85.00±3.16%), respectively. Percentage motility of groups A to D was similar but higher (P<0.05) than groups E to G. The morphological characteristics ranged between 10.88 and 12.74% in all the groups. In this study, motility of the sperm cells in groups A to D and F were above 60%. This indicated that the sperm motility in these groups was not affected by the treatment. However, groups E and G showed a reduction in percentage motility and viability. The study concluded that S. mombin fractions did not affect sperm cells structurally but treatment with ethyl acetate and methanolic fraction caused significant reduction (P<0.05) in percentage motility and viability, thus may precipitate infertility.
Testicular and epididymal parameters of spondias mombin L. (anacardiaceae) protected male wistar rats exposed to sodium arsenite
(IDOSI Publications, 2014) Ola-Davies, O.; Ajani, O. S.; Oyeyemi, M. O.; Ekundayo, S.
The testicular and epididymal protective potential of Spondias mombin. L in arsenic-treated rats was carried out. Thirty-five male albino rats (225-228g) were used and grouped into 7 (A to G), each group containing 5 rats. Group A was treated with 0.1ml Dimethysulphoxide (DMSO), B (0.1ml of Distilled water), C (Sodium arsenite; SA-2.5mg/kg body weight), D (Ethylacetate fraction), E (Ethylacetate fraction for 7 days and Sodium arsenite the 7th day), F (Methanolic fraction for 7days and Sodium arsenite the 7th day) and G (Methanolic fraction for 7 days). The results showed significant decrease (P>0.05) in the mean value of both the right and left testes length for group C (treated with Sodium arsenite only) compared to the remaining groups. It was also observed that Group G (treated with Methanolic fraction) had the lowest mean value (P>0.05) for the left and right testes weight and the left and right testicular diameter when compared to other groups. It was also observed that Group G (treated with Methanolic fraction) had the lowest mean values for all the epididymal parameters in this study. There was a significant decrease in the mean value of left epididymal weight, right and left epididymal length of group G compared to group B (treated with distilled water) and group A (treated with DMSO). It was concluded that the Methanolic fraction of Spondias mombin. L has no protective effect on the male albino rats exposed to sodium arsenite as it caused a reduction in epididymal parameters and testicular weight and diameter which may trigger testicular degeneration leading to infertility.