Browsing by Author "Ajayi, O. L."

Now showing 1 - 2 of 2

Isolation and prevalence of' pathogenic leptospira interrogans in slaughtered cattle in two abattoirs i n southwestern Nigeria
(Bundesministerium, 2011) Jagun, A. T.; Ajayi, O. L.; Ilugbo, M. O.; Olugasa, B. O.
Leptospirosis is a waterborne bacterial disease, transmitted to humans through contaminated water, usually by urine of rodents that are chronically infected by the pathogenic strains.The prevalence and incidence of leptospirosis in slaughtered cattle in relation to the risk of exposure of abattoir workers and the public consumers was determined in this study. The objective of this study ;was to evaluate the prevalence of bovine leptospirosis in slaughtered cattle in southwestern Nigeria. Kidney samples from 108 cattle were examined. Samples were .collected from Bodija abattoir in Ibadan, capital city of ;0yo State, and Lafenwa abattoir in Abeokuta, capital city :of Ogun State. Leptospira was isolated in Ellinghausen- McCullough-Johnson-Harris broth medium (EMJH). Pathogenecity test was carried out in Guinea pigs. Gross 'and histopathological lesions were observed in their kidneys. Leptospira species were isolated from 89 (82.4%) out of 108 kidneys from the slaughtered cattle. Twenty (95.2%) out of 21 kidneys and 69 (79.3%) out of 87 kidneys collected from Ibadan and Abeokuta respectively were positive. Only 31 (28.7%) (9 kidneys from Ibadan and 22 from Abeokuta) kidneys showed visible macroscopic changes, while histomorphological changes such as interstitial nephritis, tubular nephrosis and tubular protein cast were observed. Death of guinea pigs that were inoculated occurred within 24 hours to 168 hrs. The isolation of ieptospira interrogans and the pathology associated with kidneys obtained in this study indicates that cattle slaughter in public abattoir in South-western Nigeria may be sources of exposure and infection to abattoir workers and the public to leptospirosis
Spermiogram and testicular morphological studies of the buck after treatment with ethanol leaf extract of spondias mombin
(Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto-Nigeria, 2017-04) Oloye, A. A.; Ola-Davies, O. E.; Ajayi, O. L.; Oyeyemi, M. O.; Olurode, S. A.; Emikpe, B. O.
Twelve sexually matured West African Dwarf bucks from were used for the experiment. Plant extraction was by cold extraction method using hexane and ethanol as solvents. All the goats had bilaterally well descended free testicles. They were kept in standard goat pen, were served water ad libitum, centrosema plant and ration. They were stabilised for two weeks after which pre-treatment spermiogram was done followed by 14 days of oral administration of 800mg/kg ethanol leaf extract of Spondias mombin. Spermiogram was repeated after treatment. Two randomly selected goats were then castrated through a midline pre-scrotal incision for morphological study and histology of the testes and epididymides. Total spermatozoa morphological abnormalities in pre-treatment of 17.1% was significantly (p≤0.05) higher than the 10% recorded for post-treatment. Curved mid-piece (1.8%) and bent tail (1.8%) constituted the highest abnormalities post-treatment while curved tail (3.5%) was highest pre-treatment. Mean values of progressive motility and percentage liveability were significantly higher (p≤0.05) in post- treatment (96.17±3.10% and 98.25±1.36% respectively) compared to pre-treatment (80.83±11.84% and 78.75±9.56% respectively). Post-treatment sperm concentration (2.50±0.32 x109 cells/ml) compared with pre-treatment (2.32±0.36 x109 cells/ml) was not significantly different. Post-treatment gross and histological features of the bucks’ testes and epididymis were normal. The work revealed that Spondias mombin at 800mg/kgBW improved semen quality in bucks indicating its usefulness as a potential profertility agent.