Browsing by Author "Akinsomisoye, S. O."

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    Alteration in hormonal level and testicular histomorphology in rats treated with alcohol (ALC) and cannabinol (CBN)
    (Centre for Enchancing Knowledge, 2019) Akintayo, C. O.; Ajayi, T. O.; Akinsomisoye, S. O.; Faeji, C. O.; Adewumi, A. F.; Akele, Y. R.
    This study explores the effects of chronic alcohol and cannabinol exposure on the testosterone levels and the histomorphological integrity/cytoarchitecture of the rats’ testes. Adults male Wistar rats were randomly divided into control (distilled water), methanol (2mg/kg bw), alcohol (3g/kg bw), cannabinol (10mg/kg bw) and alcohol (3g/kg bw) plus cannabinol (10mg/kg bw) groups respectively. Alcohol and cannabinol were administered orally twice daily for 52 days (spermatogenic cycle in rats) and at the end of treatment, male reproductive organ (testis) was removed and cleared of adherent tissue and then fixed for histological examination. Blood samples were collected via retro-orbital sinus for hormone (testosterone) assay. Serum testosterone levels were measured using the enzyme immunoassay (E.I.A.) technique. Hormonal assay showed significant reductions in the levels of testosterone (T) (p < 0.05) in the alcohol alone, cannabinol alone and in the alcohol plus cannabinol treated groups. The Histological analysis of the treated groups showed severe reduction of the spermatogenic cells. The present study showed that following chronic alcohol and/or cannabinol administration the results showed significant reduction (p<0.05) in testosterone levels and a detrimental effect on the histomorphology of the testes. Alcohol and/or cannabinol therefore exhibit inhibitory effects causing inhibition of testosterone as observed in this study.
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    Effects of quercetin on oral administration of cannabinol and alcohol on reproductive functions in male Wistar rats
    (Centre for Enchancing Knowledge, 2019) Akintayo, C. O.; Ajayi, T. O.; Akinsomisoye, S. O.; Karga, S. V.; Ayodele, M. J.; Obuekwe, C. A.
    The male reproductive system ensures the continuity of species; however, several toxicants have demonstrated hampering effects on the structures of male reproductive system, thereby leading to reduced physiological functions. This study was carried out to investigate the effect of quercetin in experimental rats treated with alcohol and/or cannabinol. Forty-five male rats were grouped into the following: Group 1 rats were given distilled water. Group 2 rats were administered with methanol. Group 3 rats were administered with alcohol (3g/kg bw as 25%v/v). Group 4 rats were administered with cannabinol (10mg/kg bw). Group 5 rats were administered with cannabinol (10mg/kg bw) and alcohol (3g/kg bw as 25%v/v). Group 6 rats were administered with quercetin (30mg/kg bw). Group 7 rats were administered with alcohol (3g/kg bw as 25%v/v) and quercetin (30mg/kg bw). Group 8 rats were administered with cannabinol (10mg/kg bw) and quercetin (30mg/kg bw). Group 9 rats were administered with cannabinol (10mg/kg bw) plus alcohol (3g/kg bw as 25%v/v) plus quercetin (30mg/kg bw). At the end of the experiment, animals were sacrificed and their reproductive organs were harvested and weighed immediately. Results showed that rats treated with alcohol and/or cannabinol had significant decrease (p<0.05) in sperm indices, lipid peroxidation, gonadotrophic hormones and male sex hormone (testosterone); however, with quercetin co-treatment with alcohol and/or cannabinol administration the results obtained in the reproductive parameters showed ameliorative potential of quercetin in the present study.