Browsing by Author "Ewuola, M. K."

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    Insilico Analysis of Myostatin Gene in Selected Poultry Species
    (2018) Ewuola, M. K.; Akinyemi, M.O.; Osaiyuwu, H.O.
    Myostatin gene (GDF8) is a member of transforming growth-  superfamily and has been reported to act as a negative regular of skeletal muscle during myogenesis, regulation of adipocyte function in livestock species. This study was carried out to computationally investigate molecular genetic variation and categorize precise mutation in myostation gene in selected poultry species at the studied locus. A total of twenty (20) myostatin nucleotide sequences consisting of chicken (12), quail (4) and turkey (4) were retrieved from the GenBank. Functional analysis of nsSNP using PROVEAN showed three amino acid substitutions (P20Q, Y11F and G3R) in chicken, one in quail (Y100R) and three in turkey (N65P, F155W and K95A) were all returned neutral, suggesting their beneficial impacts. The information from nucleotides sequences showed the interclustering and close relatedness of members of phasianidae family (chicken, quail and turkey).
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    Single Nucleotide Polymorphisms Of Insulin-Like Growth Factor 2 (Igf-2) Gene Associated W Ith Bodyweights Of Nigerian Locally Adapted Turkey
    (2022) Ewuola, M. K.; Akinyemi, M.O.; Osaiyuwu, O.H.; Fatai, R.B.; Tiamiyu, A.K.; Oso, Y.A.A.; Salako,A.E.
    The Insulin-Like Growth Factor 2 (IGF-2) gene was studied as a candidate gene for bodyweight of three turkey populations (Black, Spotted and White strains). The primer was designed to detect single nucleotide polymorphisms of exon2 at the IGF-2 locus by polymerase chain reaction-restriction fragment length polymorphism. Co-dominant alleles A and B corresponding to genotypes AA, AB and BB were detected at the locus tested. No significant (P>0.05) associations were recorded for all the genotypes detected with bodyweights of the population utilized at weeks 3, 7, 9, 11 and 21 but bodyweights of homozygote BB genotypes across the three strains were higher than those of AA and AB genotypes. Significant differences (P<0.05) also occurred for male and female turkeys at weeks 15 and 19. No significant (P>0.05) association of different genotypes with bodyweight at other weeks were observed (P <0.05). The products amplified displayed polymorphism and the variation in exon2 of IGF-2 gene may be a molecular marker for superior growth in the turkey populations utilized.