Browsing by Author "Fabunmi, F. K."

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Antibiotic resistance patterns of bacterial isolates obtained from catfish and tilapia fish types consumed in Ibadan.
(Valahia University Press, 2018) Afolabi, F. T.; Fabunmi, F. K.
This study was carried out to identify antibiotic resistant bacteria isolated from Catfish (Clarias gariepinus) and Tilapia (Oreochromis sp.) fish types at Eleyele, University of Ibadan fish farm and Asejire all within Ibadan metropolis. Antibiotic susceptibility testing was carried out on Ninety (90) of the previously isolated bacterial isolates using the agar gel diffusion technique. Antibiotics used were Gentamycin, Ofloxacin, Nitrofurantoin, Ampicillin, Augmentin, Amoxicillin, Ceftazidime, Cefuroxime, Ciprofloxacin, Ceftriaxone, Erythromycin, Amikacin and Ticarcillin. Various level of multi drug resistance was observed among the isolates. The bacterial population identified from this study includes; Staphylococcus aureus, Salmonella sp., Shigella sp., Pseudomonas aeruginosa, Klebsiella pneumonia, Klebsiella oxytoca, Klebsiella ozaenae, Acinetobacter baumanii, Proteus vulgaris, Escherichia coli, Enterobacter aerogenes, Citrobacter freundii and Moraxella catarrhalis. Twelve (12) of the isolates were further subjected to plasmid profile analysis in which, plasmids of different sizes were found for 10 of the isolates. It was observed that only one Shigella sp., out of the Five (5) isolates had lost the plasmids and became responsible to the earlier resistant antibiotics while the other four isolates Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus maintained their resistance to the antibiotics. The spread of multidrug resistance pathogens has constituted a major impediment to the control of infectious diseases, and since this study has shown that fish samples could be a reservoir of bacteria carrying R-plasmids as well as genes responsible for resistance, therefore, the indiscriminate use of antibiotics should be discouraged and also national regulations on the use of antibacterial should be introduced and enforced.
Antibiotic resistance patterns of bacterial isolates obtained from catfish and tilapia fish types consumed in Ibadan.
(Valahia University Press, 2018) Afolabi, F. T.; Fabunmi, F. K.
This study was carried out to identify antibiotic resistant bacteria isolated from Catfish (Clarias gariepinus) and Tilapia (Oreochromis sp.) fish types at Eleyele, University of Ibadan fish farm and Asejire all within Ibadan metropolis. Antibiotic susceptibility testing was carried out on Ninety (90) of the previously isolated bacterial isolates using the agar gel diffusion technique. Antibiotics used were Gentamycin, Ofloxacin, Nitrofurantoin, Ampicillin, Augmentin, Amoxicillin, Ceftazidime, Cefuroxime, Ciprofloxacin, Ceftriaxone, Erythromycin, Amikacin and Ticarcillin. Various level of multi drug resistance was observed among the isolates. The bacterial population identified from this study includes; Staphylococcus aureus, Salmonella sp., Shigella sp., Pseudomonas aeruginosa, Klebsiella pneumonia, Klebsiella oxytoca, Klebsiella ozaenae, Acinetobacter baumanii, Proteus vulgaris, Escherichia coli, Enterobacter aerogenes, Citrobacter freundii and Moraxella catarrhalis. Twelve (12) of the isolates were further subjected to plasmid profile analysis in which, plasmids of different sizes were found for 10 of the isolates. It was observed that only one Shigella sp., out of the Five (5) isolates had lost the plasmids and became responsible to the earlier resistant antibiotics while the other four isolates Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus maintained their resistance to the antibiotics. The spread of multidrug resistance pathogens has constituted a major impediment to the control of infectious diseases, and since this study has shown that fish samples could be a reservoir of bacteria carrying R-plasmids as well as genes responsible for resistance, therefore, the indiscriminate use of antibiotics should be discouraged and also national regulations on the use of antibacterial should be introduced and enforced.
Physicochemical and microbiological quality of fresh and smoked catfish (Clarias gariepinus) and Tilapia (Oreochromis niloticus) in Ibadan, Nigeria
(Sciencedomain International, 2018) Afolabi, F. T.; Fabunmi, F. K.
"Aim: This study aimed to determine the microbiological quality of fresh and smoked Catfish (Clarias gariepinus) and Tilapia (Oreochromis niloticus) collected randomly from two fish markets (Asejire and Eleyele) and University of Ibadan fish farm. Study Design: Microbiological analyses of the samples were done using standard microbiological procedures. Place and Duration of Study: Fresh Catfish (Clarias gariepinus) and Tilapia (Oreochromis niloticus) species fish samples were collected at Eleyele, Asejire and University of Ibadan fish farm. All samples were collected randomly and placed in sterile polythene bags. The fresh fish samples were also collected in clean plastic containers and transported to the Postgraduate Laboratory of the Department of Microbiology, University of Ibadan for analyses. Methodology: The fish samples were cultured and isolates were obtained from the flesh, gills and guts. The pH was determined by weighing 3g of the fish samples and the samples were washed in sterile distilled water. The fish samples were also analyzed to determine their proximate composition. The fish samples were analysed using standard microbiological procedures. Results: A total of 90 bacterial isolates were obtained from the different fish samples with their bacterial count ranging from 1.0 × 102 – 5.0 × 105 CFU/g. The bacterial diversity were; Staphylococcus aureus, Salmonella sp., Shigella sp., Pseudomonas aeruginosa, Klebsiella pneumonia, Klebsiella oxytoca, Klebsiella ozaenae, Acinetobacter baumanii, Proteus vulgaris, Escherichia coli, Enterobacter aerogenes, Citrobacter freundii and Moraxella catarrhalis. A total of 51 fungal isolates were obtained also with the fungal counts ranging from 1.0 × 102 – 6.0 × 104 CFU/g. The three (3) species obtained from the samples were identified as Aspergillus sp., Fusarium sp. and Penicillium sp. The frequency of occurrence of the bacterial isolates was highest (27%) for Shigella sp. and least (1%) for both Proteus vulgaris and Pseudomonas aeruginosa. Conclusion: In the current study the fish samples obtained from the different locations could be contaminated with pathogenic microorganisms and therefore Catfish and Tilapia fish should be properly washed, cooked and smoked before consumption."