Browsing by Author "Fasuyi, O. C."

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Antimicrobial activity of crude extact of Grewia Mollis Smith (Malvaceae) on clinical isolates of Escherichia Coli from cases of Diarrhea
(College of Medicine and Allied Health Sciences, University of Sierra Leone, 2021) Okunye, O. L.; Idowu, P. A.; Ike, W. E.; Ojieabu, W. A.; Fasuyi, O. C.
Background: Escherichia coli is one of the leading causes of primary intestinal infections, particularly diarrhoea and other types of opportunistic infections of humans. The antimicrobial, phytochemical contents and minimum inhibitory concentration of Grewia mollis Smith (Malvaceae) were determined in a quest to evaluate their potentials as sources of alternative medicine for the treatment of diarrhoea. Methods: The bacteriological investigation was carried out on 25 clinical isolates of Escherichia coli from cases of diarrhoea which includes; sub-culturing onto eosin methylene blue agar and incubated at 370C for 24 hours. Gram staining, lactose fermentation, indole formation from tryptophan, gelatin liquefaction and Voges - Proskauer test were carried out on the isolates. The antimicrobial, phytochemical analysis and minimum inhibitory concentration (MIC) of Grewia mollis extract were determined using Evans and Trease method. Results: Grewia mollis leave methanol, ethyl acetate and N- hexane extracts gave extraction yield 8.40, 3.86 and 2.55 respectively. Tannins, saponins, flavonoids and alkaloids were detected as bioactive compounds from the leave of Grewia mollis investigated. The minimum inhibitory and minimum bactericidal concentration of the extract elicited remarkable antimicrobial activity in correlation to the polarity of each solvent.
Antimicrobial Activity of Crude Extract of Grewia mollis Smith (Malvaceae) on Clinical Isolates of Escherichia coli from Cases of Diarrhoea
(College of Medicine and Allied Health Sciences, University of Sierra Leone, 2021) Okunye, O. L.; Idowu, P. A.; Ike, W. E.; Ojieabu, W. A.; Fasuyi, O. C.
Background: Escherichia coli is one of the leading causes of primary intestinal infections, particularly diarrhoea and other types of opportunistic infections of humans. The antimicrobial, phytochemical contents and minimum inhibitory concentration of Grewia mollis Smith (Malvaceae) were determined in a quest to evaluate their potentials as sources of alternative medicine for the treatment of diarrhoea. Methods: The bacteriological investigation was carried out on 25 clinical isolates of Escherichia coli from cases of diarrhoea which includes; sub-culturing onto eosin methylene blue agar and incubated at 370C for 24 hours. Gram staining, lactose fermentation, indole formation from tryptophan, gelatin liquefaction and Voges–Proskauer test were carried out on the isolates. The antimicrobial, phytochemical analysis and minimum inhibitory concentration (MIC) of Grewia mollis extract were determined using Evans and Trease method. Results: Grewia mollisleave methanol, ethyl acetate and N- hexane extracts gave extraction yield 8.40, 3.86 and 2.55 respectively. Tannins, saponins, flavonoids and alkaloids were detected as bioactive compounds from the leave of Grewia mollis investigated. The minimum inhibitory and minimum bactericidal concentration of the extract elicited remarkable antimicrobial activity in correlation to the polarity of each solvent. Conclusion: Therefore, Grewia mollis leave extracts hold the potential for clinical application.
Assessment of the antimicrobial activity of lactic acid bacteria from sorghum slurry on Clinical Strains of Diarrhoeagenic Escherichia coli
(Antibacterial activity, Diarrhoeagenic Escherichia coli, Lactic acid bacteria, Lactobacillus plantarum, Sorghum slurry., 2021) Okunye, O. L.; Okanlawon, B. M.; Idowu, P. A.; Adeleye, O.; Fasuyi, O. C.
Background: Sorghum is a nutrient-rich grain ground into flour to make different types of delicacies, and it has been reported to possess probiotic potentials. Objective: To assess the antimicrobial activity of Lactobacillus plantarum obtained from sorghum slurry on strains of enteropathogenic Escherichia coli from cases of diarrhoea. Methods: A total of 36 samples of wet-milled sorghum slurry and liquor pH were obtained and cultured on MRSA and were, after that, biochemically characterized for Lactobacillus plantarum, which was tested by agar well diffusion against 15 strains of Escherichia coli isolated from cases of diarrhoea. Results: Microbiological analysis of the 36 samples of sorghum explored produced 15 isolates of Lactobacillus plantarum. A progressive increase in acidity in relation to an increase in the period of fermentation was observed. Ninety-five per cent of the Escherichia coli strains showed resistance against some standard antibiotics. At the same time, the isolates of Lactobacillus plantarum obtained inhibited isolates of diarrhoeagenic Escherichia coli tested, showing potential usefulness of the sorghum slurry as a probiotic. Conclusion: The inhibitory activity of Lactobacillus plantarum isolated from sorghum slurry showed antimicrobial potentials that could be used for therapeutic purposes in treating diarrhoea caused by Escherichia coli, pending further investigation.
Assessment of the antimicrobial activity of lactic acid bacteria from sorghum slurry on clinical strains of diarrhoeagenic Escherichia coli.
(Dental Consultants Association of Nigeria, OOUTH, Sagamu, Nigeria, 2021) Okunye, O. L.; Okanlawon, B. M.; Idowu, P. A.; Adeleye, O.; Fasuyi, O. C.
Background: Sorghum is a nutrient-rich grain ground into flour to make different types of delicacies, and it has been reported to possess probiotic potentials. Objective: To assess the antimicrobial activity of Lactobacillus plantarum obtained from sorghum slurry on strains of enteropathogenic Escherichia coli from cases of diarrhoea. Methods: A total of 36 samples of wet-milled sorghum slurry and liquor pH were obtained and cultured on MRSA and were, after that, biochemically characterized for Lactobacillus plantarum, which was tested by agar well diffusion against 15 strains of Escherichia coli isolated from cases of diarrhoea. Results: Microbiological analysis of the 36 samples of sorghum explored produced 15 isolates of Lactobacillus plantarum. A progressive increase in acidity in relation to an increase in the period of fermentation was observed. Ninety-five per cent of the Escherichia coli strains showed resistance against some standard antibiotics. At the same time, the isolates of Lactobacillus plantarum obtained inhibited isolates of diarrhoeagenic Escherichia coli tested, showing potential usefulness of the sorghum slurry as a probiotic. Conclusion: The inhibitory activity of Lactobacillus plantarum isolated from sorghum slurry showed antimicrobial potentials that could be used for therapeutic purposes in treating diarrhoea caused by Escherichia coli, pending further investigation.