Browsing by Author "Fatai, R.B."

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Genetic Variation In Indigenous Turkey Populations In South West Nigeria
    (2017) Fatai, R.B.; Akinyemi, M.O.; Osaiyuwu,O. H.
    The study was carried out to assess the genetic variation in indigenous turkey populations in South West Nigeria using seven protein markers: Hemoglobin (Hb), Albumin (Alb), Transferin (Tf), Esterase-1 (Es-1), Alkaline phosphatase (ALP), Carbonic anhydrase (CA), and Esterase-3 (Es-3).. A total of 97 turkey birds from Ibadan, Ijebu Ode and Akure were purposively sampled. Separation of plasma and red cell proteins were determined on cellulose acetate electrophoresis to estimate the genetic variability within and among the populations. The degree of heterozygosity, deviation from Hardy Weinberg’s Equilibrum (HWE), FIS, and FST values were estimated. From a total of 7 loci analyzed, six were found to be polymorphic in all the populations sampled while Es-1 was monomorphic. Two codominant alleles which controlled three different genotypes were observed at each polymorphic loci. Deviations from Hardy Weinberg equilibrium and a deficiency of heterozygotes were observed in all the populations. Average heterozygosities ranged between 0.13 and 0.20, heterozygote deficit FIT was estimated at 0.5784 while within breed deficit as evaluated by FIS ranged from 0.38 to 0.56. The fixation index FST revealed that genetic diversity within the studied population was moderately differentiated. Genetic distance among the populations quantified through calculation of Nei genetic distance ranged from 0.037 - 0.109. The result obtained may be used as an initial guide in defining objectives for future investigations of genetic integrity and developing conservation strategies for Nigeria indigenous turkeys.
  • Thumbnail Image
    Item
    Single Nucleotide Polymorphisms Of Insulin-Like Growth Factor 2 (Igf-2) Gene Associated W Ith Bodyweights Of Nigerian Locally Adapted Turkey
    (2022) Ewuola, M. K.; Akinyemi, M.O.; Osaiyuwu, O.H.; Fatai, R.B.; Tiamiyu, A.K.; Oso, Y.A.A.; Salako,A.E.
    The Insulin-Like Growth Factor 2 (IGF-2) gene was studied as a candidate gene for bodyweight of three turkey populations (Black, Spotted and White strains). The primer was designed to detect single nucleotide polymorphisms of exon2 at the IGF-2 locus by polymerase chain reaction-restriction fragment length polymorphism. Co-dominant alleles A and B corresponding to genotypes AA, AB and BB were detected at the locus tested. No significant (P>0.05) associations were recorded for all the genotypes detected with bodyweights of the population utilized at weeks 3, 7, 9, 11 and 21 but bodyweights of homozygote BB genotypes across the three strains were higher than those of AA and AB genotypes. Significant differences (P<0.05) also occurred for male and female turkeys at weeks 15 and 19. No significant (P>0.05) association of different genotypes with bodyweight at other weeks were observed (P <0.05). The products amplified displayed polymorphism and the variation in exon2 of IGF-2 gene may be a molecular marker for superior growth in the turkey populations utilized.