Browsing by Author "Fukushi, Y."

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Anti-sickling activities of two isolated compounds from the root of Combretum racemosum P. beauv. (Combretaceae)
(Elsevier Inc., 2021) Famojuro, T. I.; Ajayi, T. O.; Fasola, F. A.; Fukushi, Y.; Omotade, O. O.; Moody, J. O.; Adeyemi, A. A.
Ethnopharmacological relevance: Evaluation of plants such as Combretum racemosum with claimed traditional use in the management of sickle cell anaemia in Nigeria and other parts of West Africa could serve as a useful research strategy in the search for potential anti-sickling drugs and templates. Aim of the study: This study aimed at evaluating the antisickling potential of C. racemosum by activity-guided purification and isolation of its active constituents. Materials and methods: Crude methanol extract of the root of C. racemosum and the fractions obtained by par titioning with chloroform, ethyl acetate, and aqueous were investigated for anti-sickling activity against sodium metabisulphite induced sickling of sickle cell haemoglobin (HbSS). Repeated chromatographic separations were conducted on the most active chloroform fraction to purify and isolate bioactive compounds for further tests for anti-sickling activity. The characterization of the isolated compounds was done by mass spectrometry (FD+MS) and nuclear magnetic resonance ( 1 HNMR) spectroscopy. Results: The chloroform fraction (FA) (% sickled erythrocyte ranged from 3.0 to 34.1) exhibited better anti sickling activity than aqueous (% sickled erythrocyte ranged from 38.9 to 51.5) as well as the crude methanol (% sickled erythrocyte ranged from 19.1 to 30.4). Hence, the phytochemical investigation was focused on the chloroform fraction, which led to the identification of two ellagic acid derivatives (3,3′ ,4′ -tri-O-methyl ellagic acid (A) and 3,3′ -di-O- methyl ellagic acid (B). The two isolated compounds possessed good, comparable anti sickling activities with compound A exhibiting a slightly better in vitro activity. Conclusion: This paper reports for the first time anti-sickling principles from C. racemosum and therefore, provided some justification for the ethnomedicinal use of the plant in the management of sickle cell disease.
Antimicrobial activity of garcinia kola (Heckel) seed extracts and isolated constituents against caries-causing microorganisms
(2014-09) Ajayi, T. O.; Moody, J. O.; Fukushi, Y.; Adeyemi, T. A.; Fakeye, T. O.
Garcinia kola Heckel (Guttiferae) seed, has found use in folkloric medicine of Southern Nigeria for the treatment of toothache and prevention of dental caries. The crude ethanolic extract, chromatographic fractions and isolated constituents of Garcinia kola seed against clinical strains of dental-caries-causing and related microorganisms is being evaluated. Antimicrobial evaluations were done by testing different concentrations of the crude extract, vacuum liquid chromatographic (VLC) fractions and pure isolates against Streptococcus mutans, Streptococcus viridans and Staphylococcus aureus in already set blood agar with gentamicin as the reference standard. The zones of inhibition and minimum inhibitory concentrations (MIC) were determined as appropriate. Fraction N, eluted with (hexane: ethyl acetate 70: 30), exhibited the highest activity with MIC’s of 1.50 mgml⁻¹ and 0.33 mgml⁻¹ while the pure isolates 1 (cycloartenol) and 2 (24-methylenecycloartanol) gave MIC’s of 0.17 mgml⁻¹ and 0.38 mgml⁻¹ against Streptococcus mutans and Streptococcus viridans respectively. Isolate 3 (garcinianin) gave MIC of 1.0 mgml⁻¹ against Streptococcus mutans but there was no significant activity against Streptococcus viridans and Staphylococcus aureus. The results provide justifications for the folkloric use of Garcinia kola Heckel (Guttiferae) for dental caries-related health problems while the isolated compounds may also serve as templates for future antimicrobial drug development.
Antimicrobial activity of Garcinia kola (heckel) seed extracts and isolated constituents against caries-causing microorganisms
(Ibadan Biomedical Communications Group, 2014) Ajayi, T. O.; Moody, J. O.; Fukushi, Y.; Adeyemi, T. A.; Fakeye, T. O.
Garcinia kola Heckel (Guttiferae) seed, has found use in folkloric medicine of Southern Nigeria for the treatment of toothache and prevention of dental caries. The crude ethanolic extract, chromatographic fractions and isolated constituents of Garcinia kola seed against clinical strains of dental-caries-causing and related microorganisms is being evaluated. Antimicrobial evaluations were done by testing different concentrations of the crude extract, vacuum liquid chromatographic (VLC) fractions and pure isolates against Streptococcus mutans, Streptococcus viridans and Staphylococcus aureus in already set blood agar with gentamicin as the reference standard. The zones of inhibition and minimum inhibitory concentrations (MIC) were determined as appropriate. Fraction N, eluted with (hexane: ethyl acetate 70: 30), exhibited the highest activity with MIC’s of 1.50 mgml⁻¹ and 0.33 mgml⁻¹ while the pure isolates 1 (cycloartenol) and 2 (24-methylenecycloartanol) gave MIC’s of 0.17 mgml⁻¹ and 0.38 mgml⁻¹ against Streptococcus mutans and Streptococcus viridans respectively. Isolate 3 (garcinianin) gave MIC of 1.0 mgml⁻¹ against Streptococcus mutans but there was no significant activity against Streptococcus viridans and Staphylococcus aureus. The results provide justifications for the folkloric use of Garcinia kola Heckel (Guttiferae) for dental caries-related health problems while the isolated compounds may also serve as templates for future antimicrobial drug development.