Browsing by Author "Gbadegesin, M. A."

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An overview of the genetics of cervical cancer.
(2018) Gbadegesin, M. A.; Soremekun, O.; Oluwasola, T. A. O.; Okolo, C. A.; Oluwasola, A. O.
Cervical cancer is the fourth most common cancer in women, and the seventh of all human cancers. It is the most rampant cancer of the female genital tract in the developing world and manifests in two common histological subtypes: squamous cell carcinoma which is derived from squamous cells of the cervix and cervical adenocarcinoma which arose from the glandular cells. Most cases of deaths from cervical cancer occur in the less developed countries of the world where there are ineffective screening systems. Factors that increase the risk for developing cervical cancer include infection by Human Papilloma Virus (HPV) as the main direct factor and other indirect factors such as smoking, dietary habits, age, race, socioeconomic status, sexual history, use of oral contraceptives, high parity and the human immunodeficiency virus infection. Identifying the genetic alterations that predispose to or associate with cervical cancer will help in the screening of patients at risk of the cancer thereby allowing early diagnosis and prompt management with better outcomes. In this review we describe the role of HPV DNA integration into the host cellular genome, the effects of viral E6 and E7 proteins, and the loss of heterozygosity as genetic factors in cervical cancer.
Mutation analysis of p53 gene in cervical cancer and useful polymorphic variants in exons 3 and 4
(2021) Gbadegesin, M. A.; Omotosho, O. E.; Oluwasola, T. A. O.; Okolo, C. A.; Oluwasola, A. O.; Soremekun, O.; Ogun, G. O.; Abideen O. O.; Oyeronke A. O.
Background: Factors contributing to the pathogenesis and progression of cervical cancer include poor attitude to screening and health intervention, late presentation, among others. Mutations in p53 gene have been attributed to several cancer cases. The present study was designed to find relationships between the mutation patterns in p53 gene and cervical carcinoma staging. Such knowledge could contribute to early diagnosis of cervical cancer. Results: From the sequence analysis of p53 gene fragment isolated by polymerase chain reactions (PCR), nineteen (19) polymorphic variants were identified. Missense mutations occurred in 47% of the samples, 32% were silent mutations, 16% were frameshift mutations and 5% nonsense mutations. Socio-biological characteristics of the study participants revealed that 60% have husbands with multiple sexual partners and that only 23.3% of the participants have ever had the Papanicolaou (Pap) smear test prior to diagnosis, whilst 20% were unaware of the screening test. Conclusions: Increased severity of cervical carcinoma staging as revealed from the histopathological analysis was found to be associated with accumulation of higher levels of mutations in the p53 gene. Molecular analysis of p53 gene mutations may prove useful as a screening biomarker for cervical cancer.