Browsing by Author "Ogbole, O. O."

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Anti-inflammatory and antimicrobial activities of hippocratea indica root bark and poga oleosa fruits
(2007) Ogbole, O. O.; Ekor, M. N.; Oluremi, B. B.; Ajaiyeoba, E. O.; Gbolade, A. A.; Ayoola, M. A.; Adeyemi, A. A.
The methanolic extracts of Hippocratea indica root bark and Poga oleosa fruits were investigated for anti-inflammatory and antimicrobial activities. Both extracts inhibited carrageenan-induced paw oedema significantly in rats in a dose-dependent manner in 4 h. H. indica inhibited oedema significantly at the minimal dose (125 mg/ml, p< 0.05) from 2 h onward, and gave 100% inhibition in 4 h. at 250 mg/kg. It was shown to be a more potent anti-inflammatory agent than P. oleosa. Also, H. indica extract exhibited greater antimicrobial activity against tested bacteria, with Staphylococcus aureus being the most susceptible bacterium (MIC, 16 mg/ml). Both plants were inactive against Candida albicans. These results confirm the anti-inflammatory and antibacterial activities of the two plants.
Evaluation of the antibacterial and antifungal potentials of peptide-rich extracts from selected Nigerian plants
(Elsevier, 2022) Agbebi, E. A.; Alabi, O. S.; Nkrumah, A. O.; Ogbole, O. O.
Introduction: Host defence peptides (HDPs) has gained wide recognition as a potential source of antimicrobial agents because of their abundance in nature, low incidence of reported resistance, and their immunomodulatory properties. This study investigated the presence and antimicrobial activities of bioactive peptides in seven selected plants from the Rubiaceae, Apocynaceae, and Euphorbiaceae families. Methods: The reversed-phase solid-phase extraction was used to obtain the peptide-rich fractions from the crude extracts of the plants, and the circular peptides were chemically detected by a modified G-250 spray on a developed TLC plate. The peptide fraction and crude extracts were screened for their antimicrobial activities using the broth microdilution method. The rate of kill study was conducted for the peptide fractions Euphorbia hirta and Nauclea diderichii Results: The study revealed Nauclea diderichii peptide-rich fraction as the most potent on the tested strains of gram-positive and gram-negative bacteria and fungi (MIC =7.8, 15.63-62.5, and 7.8 μg/mL respectively). Euphorbia hirta and Nauclea diderichii peptide fractions exhibited bactericidal activity (MIC index ≤4, and >3Log10 reduction of initial inoculum) and have a broad spectrum of activity, which further validates their ethnomedicinal use in the management of diverse infectious diseases, including enteric and respiratory tract infections. The peptide fractions of all the plants are more potent than the crude extracts. Conclusion: This study established the antimicrobial potentials of the peptide-rich fractions from these plant species, and the basis for further investigation to isolate and characterize the bioactive peptides responsible for their antimicrobial activities.
In vitro antiviral activity of peptide‑rich extracts from seven Nigerian plants against three non‑polio enterovirus species C serotypes
(Springer Nature, 2021) Ogbole, O. O.; Akinleye, T. E.; Nkumah, A. O.; Awogun, A.O.; Attah, A. F.; Adewumi, M. O.; Adeniji, A.J.
Background: As frequent viral outbreaks continue to pose threat to public health, the unavailability of antiviral drugs and challenges associated with vaccine development underscore the need for antiviral drugs discovery in emergent moments (endemic or pandemic). Plants in response to microbial and pest attacks are able to produce defence molecules such as antimicrobial peptides as components of their innate immunity, which can be explored for viral therapeutics. Methods: In this study, partially purified peptide-rich fraction (P-PPf ) were obtained from aqueous extracts of seven plants by reverse-phase solid-phase extraction and cysteine-rich peptides detected by a modified TLC method. The peptide-enriched fractions and the aqueous (crude polar) were screened for antiviral effect against three non-polio enterovirus species C members using cytopathic effect reduction assay. Results: In this study, peptide fraction obtained from Euphorbia hirta leaf showed most potent antiviral effect against Coxsackievirus A13, Coxsackievirus A20, and Enterovirus C99 (EV-C99) with IC50< 2.0 μg/mL and selective index ≥ 81. EV-C99 was susceptible to all partially purified peptide fractions except Allamanda blanchetii leaf. Conclusion: These findings establish the antiviral potentials of plants antimicrobial peptides and provides evidence for the anti-infective use of E. hirta in ethnomedicine. This study provides basis for further scientific investigation geared towards the isolation, characterization and mechanistic pharmacological study of the detected cysteine-richpeptides.
Medicinal Importance and Phytoconstituents of Underutilized Legumes from the Caesalpinioideae DC Subfamily
(MDPI, 2023-08) Ogunniyi, Q. A.; Ogbole, O. O.; Akin-Ajani, O. D.; Ajala, T. O.; Olorunsola, B.; Fettke, J.; Odeku, O. A.
Underutilized legumes are common crops in developing countries with superior dietarypotentials that could be useful sources of protein as well as some phytoconstituents. They are more tolerant of abiotic environmental conditions like drought than the major legumes. This makes them more adapted to harsh soil and climatic conditions, which helps to minimize the pressure brought on by climate change. However, despite their potential, underutilized legumes have been greatly overlooked compared to the major legumes due to supply constraints. Underutilized legumes in the subfamily Caesalpinioideae are better suited for use as animal feeds with little or no value as food for humans, and the extracts and infusions of the different parts of plant species in this subfamily are traditionally used for the treatment of different diseases. In addition, underutilized legumes in this subfamily contain phytoconstituents that are of pharmacological relevance, some of which have been isolated, characterized and evaluated for use in the treatment of a variety of disorders. Therefore, this review describes the medicinal activities of some selected underutilized legumes from five genera in the subfamily Caesalpinioideae as well as their phytoconstituents, which could be exploited as lead compounds for drug discovery
Molecular identity and cytotoxicity of Lenzites quercina macrofungus extracts toward cancer cell lines
(2017) Ogidi, O. C.; Oyetayo, V. O.; Akinyele, B. J.; Ogbole, O. O.; Adeniji, J. A.; Oluremi B. B.
The medicinal uses of wild macrofungi have been attributed to their accumulated bioactive compounds. Several mushrooms have been reported to possess antitumor activity, but little, in this regard, is known about Lenzites quercina collected from Akure in Nigeria. Hence, the molecular identification and cytotoxic activity of extracts obtained from raw and fermented Lenzites quercina were assessed. The macrofungus Lenzites quercina was identified using Internal Transcribed Spacers (ITS) sequence analysis. The basic local alignment search tool (BLAST) analyzed on NCBI GenBank data revealed that the Lenzites species from Nigeria – accession number, JF689829.1 – was closely related to Lenzites quercina (a 100% relationship match). The cytotoxic activity of raw and fermented Lenzites quercina extracts was tested against human cervical cancer (HeLa), rhabdomyosarcoma (RD) and African green monkey kidney (VERO) cell lines. A tetrazolium yellow 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay was used to evaluate the reduction in viability of cell cultureswith or without the extracts of Lenzites quercina. Extracts of Lenzites quercina exhibited cytotoxic activity (6.0-84.5%) against the tested cancer cell lines (HeLa, VERO and RD). The concentration of the bioactive compounds in the crude extract ranged from 0.01 to 1000 μg/ml. The results revealed that bioactive compounds in Lenzites quercina possess cytotoxic properties. These bioactive compounds may be isolated and used as alternative therapies to currently available anticancer drugs.
Peptide-rich extracts from leaves of newbouldia laevis (P. Beauv.) seem. ex. bureau (bignoniaceae) with antimicrobial and brine shrimp lethality activities
(Faculty of Pharmaceutical Sciences, University of Jos, Nigeria, 2024-05) Nkumah, A. O.; Kehinde, C. T.; Oluremi, B. B.; Attah, A. F.; Ogbole, O. O.
Global concerns over antibiotic-resistant microbial pathogens have spurred a quest for innovative, stable, and target-specific molecules like bioactive peptides. This study evaluates the antimicrobial properties and toxicity of peptide-enriched extracts from Newbouldia laevis leaves. Aqueous extraction, thin layer chromatography (TLC), and Phenomenex's Stratum Giant Tube C18-E were used to obtain partly purified fraction (PPF) and crude peptide extracts (CPE). Antimicrobial inhibitory capacity was determined using p-INT dye, with gentamicin and fluconazole as standards. Cytotoxicity was assessed with Artemia salina nauplii. Microsoft Excel and GraphPad Prism 5 was used in analysing the data. Peptides were detected in CPE and PPF via TLC and modified G-250. PPF demonstrated more efficacy (IC50: 5.89μg/mL to 33.94μg/mL) against bacteria and fungi, with low toxicity (LC50: 5964.87μg/mL for PPF and 1094.20μg/mL for CPE) in the Brine shrimp lethality assay. The study presents the discovery of bioactive peptides from the leaves of Newbouldia laevis. Consequently, bioactive peptides extracted from this plant hold potential as foundational compounds for the development of novel broad-spectrum antibiotics aimed at combating microbial infections.
Phytochemical and antimicrobial screening of the leaf extract of diospyros barteri. Gurke.
(2010-08) Oluremi, B. B.; Osungunna, M. O.; Ogbole, O. O.
Diospyrous bateri Gurke family Ebenaceae, has been commonly used by traditional medical practitioners as anti-nfectious agent. In this study, preliminary screening of phytochemical constituents of D. barteri was carried out in addition to evaluating the antimicrobial activity of four different extracts of this leaf on some common bacteria species. The antimicrobial activity of methanol, chloroform, hexane and aqueous extracts of the leaves were tested using the agar diffusion method on two Gram positive, three Gram negative bacteria viz; Staphylococcus aureus, Proteus vulgaris, Klebsiella pneumoniae Escherichia coli, and Pseudomonas aeruginosa, The preliminary phytochemical screening revealed the presence of alkaloids, saponins and tannins while other secondary metabolites were absent. The study amply demonstrated the broad-spectrum antimicrobial property of the polar extracts while the non polar extract exhibits no activity. The minimum inhibitory concentration of the methanol extract on Proteus vulgaris, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus were 1.20mg/ml, 2.24mg/ml, 3.55mg/ml, 9.12mg/ml and 10.0mg/ml respectively, while the minimum inhibitory concentration of the water extract on Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were 8.91mg/ml, 9.55mg/ml, 10.59mg/ml, 10.96mg/ml and 71.78mg/ml respectively.