Browsing by Author "Okoli, S. U."

Now showing 1 - 5 of 5

Hypogonadism in males exposed to mixed chemicals in a mechanic village in Bodija, Ibadan
(2015) Okoli, S. U.; Charles-Davies, M. A.; Onifade, A. A.; Adekola, S.
Africa has great intensity of chemical exposure and high level of infertility. Functional disorders of the male germ cell and endocrinopathies have been attributed to exposure to mixed chemicals. Data on the mixed chemical exposure on reproductive hormones are sparse in Nigeria. This study was designed to evaluate male reproductive hormones and determinants of occupational exposure to mixed chemicals in a mechanic village in Ibadan. Forty-three males, auto mechanics aged 18-60 years occupationally exposed to mixed chemicals (MCG) at their work place in Bodija mechanic village with mean±SEM duration of 21.2±1.9 years were age matched with 40 unexposed males (controls) from the University College Hospital, Ibadan and environs. Demography, anthropometry, blood pressure and sexual history were obtained by standard methods. Blood (10 ml) was obtained from each participant for estimation of reproductive hormones by enzyme linked immunosorbent assay while total antioxidant capacity (TAC) was determined spectrophotometrically. Testosterone/oestradiol ratio (TE ratio) was calculated. The MCG were classified based on their reproductive hormone levels into 4 subgroups as eugonadic, hypogonadotrophic hypogonadism, sub-optimal hypogonadism and compensated hypogonadism. P<0.05 was regarded as significant. There were significantly raised oestradiol levels, diastolic blood pressure, waist circumference and waist to hip ratio and significantly lower TAC and TE ratio in the MCG than the controls (P<0.01). Exposed hypogonadic males exhibited significantly lower LH, FSH, testosterone, prolactin, TAC levels and TE ratio than exposed eugonadic males (P<0.05). Hypogonadism in the males exposed to mixed chemicals may be associated with reduced TE ratio resulting from increased adipose mass as well as oxidative stress associated with the mixed chemical exposure.
Inhibin B levels in relation to obesity measures and lipids in males with different numbers of metabolic syndrome components
(2016) Laniyan, D. O.; Charles-Davies, M. A.; Fasanmade, A. A.; Olaniyi, J. A.; Oyewole, O. E.; Owolabi, M. O.; Adebusuyi, J. R.; Hassan, O.; Ajobo, B. M.; Ebesunun, M. O.; Adigun, K.; Akinlade, K. S.; Okoli, S. U.; Arinola, O. G.; Agbedana, E. O.
Introduction: Defective spermatogenesis and metabolic syndrome affect 2-4% and 12.4% of males respectively. Deficient testosterone levels due to increased conversion of testosterone to oestradiol have been demonstrated in males with the metabolic syndrome (MS) with limited pituitary and leptin contribution. Defective spermatogenesis is thus implicated in males with MS but is controversial. Inhibin B is a marker of spermatogenesis. This study aims at evaluating inhibin B levels and their relationship with obesity measures and lipids in males with different number of MS components. Materials and Methods: This is a preliminary prospective study in which a total of 106 apparently healthy males (30, 30, 30 and 16 males with 0, 1, 2 and ≥3 components of metabolic syndrome (NMSC) respectively) aged 19-64 years were purposely selected. Blood pressure (BP) and obesity measures (including visceral adiposity index (VAI) and body mass index (BMI)) were obtained by standard methods. Fasting plasma glucose (FPG), total cholesterol (TC), triglycerides and high density lipoprotein cholesterol (HDLC) were determined by enzymatic methods while low density lipoprotein cholesterol (LDLC) and the lipid ratios (TG/HDLC, TC/HDLC, LDLC/HDLC) were calculated. Inhibin B was analysed by enzyme linked immunosorbent assay (RayBiotech, Inc. USA). Data analysed using analysis of variance (ANOVA) and multiple regressions were significant at P <.05. Results: Inhibin B decreased significantly in males with 0 to 2 NMSC (P <.05). However, inhibin levels between males with 0 and ≥3 NMSC were similar. Age and inhibin B levels were also similar among the different classes of BMI (P>0.05). Inhibin B related positively with HDLC and TC but negatively with VAI, LDLC and TC/HDLC. Conclusion: Reproductive function appears protected in Nigerian males with MS. However, improvement in HDLC, LDLC, TC levels, VAI and TC/HDLC may enhance fertility potential especially in males with one or two MS components, probably through dietary modulation and physical activity.
Oxidative stress biomarkers and their relationship with testosterone in male auto mechanics in Ibadan, Nigeria
(Sciencedomain International, London, 2016) Adekola, S. A.; Charles-Davies, M. A.; Onifade, A. A.; Okoli, S. U.
Occupational exposure to mixed chemicals generates free radicals with inadequate antioxidants resulting in oxidative stress. Recently, hypogonadism in male auto-mechanics was associated with oxidative stress. Studies show that testosterone, a male hormone increases the activities of antioxidant enzymes. This study is aimed at evaluating the oxidative stress biomarkers and their relationship with testosterone in auto mechanics in Ibadan, Nigeria. Eighty-three males participated in this prospective cross sectional study after informed consent. Forty-three were male auto-mechanics, occupationally exposed to mixed chemicals in the mechanic community, Bodija, Ibadan (cases). Their mean (SEM) age and body mass index (BMI) were 42.5 (1.7) years and 23.8 (0.5) Kg/m2 respectively. They were age and BMI matched with 40 unexposed, apparently healthy males from the University College Hospital and environs (controls). Demography, social habits, anthropometry and gonadal status were obtained by standard methods. Serum obtained from blood (10 ml) collected from the participants was used for biochemical analyses. Testosterone levels were determined by enzyme immunoassay method (Immunometrics UK Ltd). Levels of total antioxidant capacity, total plasma peroxide (TPP), malondialdehyde (MDA), hydrogen peroxide (H2O2), glutathione peroxidase (GPX), superoxide dismutase (SOD), glutathione-S-transferase (GST), and reduced glutathione (GSH) were determined using spectrophotometric methods while oxidative stress index (OSI) was calculated. P<0.05 was regarded as significant. TPP, MDA, OSI, H2O2 and GST `levels were significantly higher (P<0.001) in eugonadal cases compared with controls. All these biomarkers levels were similar in hypogonadal compared with eugonadal cases. (P>0.05) Testosterone related negatively with SOD in the controls only but positively with MDA and negatively with GST in cases only (P<0.05). Occupationally exposed auto mechanics appear to have oxidative stress and may benefit improvement in antioxidant status. Testosterone may contribute to and enhance total antioxidant status, which may be important in gonadal function.
Relationship between testosterone, oxidative stress biomarkers and antioxidant levels in male auto-mechanics in Ibadan, Nigeria
(Biomedical Communications Group, Ibadan, 2016) Balogun, A. M.; Charles-Davies, M. A.; Chikezie, I. C.; Okoli, S. U.
Hypogonadism attributable to males with metabolic syndrome was observed in automechanics occupationally exposed to mixed chemicals accompanied by oxidative stress (OS). We evaluated association between testosterone, OS biomarkers, enzymatic and non-enzymatic antioxidants in normal weight automechanics in Ibadan. This was a prospective cross sectional study involving 100 normal weight males aged 18 – 60 years. They were 50 automechanics in Ibadan, age and anthropometry matched with 50 eugonadic males from University College Hospital and environs (controls). Demographic, anthropometry, social habits and dietary history were obtained by standard methods. Blood (10mL) was collected and serum/plasma was used for biochemical analyses. Enzymatic antioxidants (catalase, glutathione peroxidase, superoxide dismutase (SOD) and glutathione -S- transferase (GST); non-enzymatic antioxidants (reduced glutathione (GSH), selenium and zinc), OS biomarkers (hydrogen peroxide (H2O2), malondialdehyde (MDA), total antioxidant capacity (TAC), total plasma peroxides (TPP) and oxidative Stress index (OSI) were estimated spectrophotometrically. Testosterone was assayed by enzyme immunoassay method (Dialab, Austria). Student’s t-test, Chi-square test and multiple regression were used for comparisons, associations and relationships respectively, which were significant at P<0.05. Testosterone, TPP, OSI, GST, MDA, H2O2, selenium and zinc concentrations were significantly higher while catalase and SOD concentrations were lower in automechanics than controls (P<0.05). However, testosterone levels in both groups were within the normal reference interval. TAC, OSI and GSH had significantly negative relationship while TPP had positive relationship with years at occupation in automechanics only (P<0.05). Automechanics may have OS but not hypogonadism probably due to increased antioxidant intake.
Reproductive function in premenopausal African blacks with metabolic syndrome: associations among Inhibin B, adipokines, pituitary and sex hormones and sex hormone binding globulin
(2016) Famuyiwa, I. O.; Bitrus, D. P.; Charles-Davies, M. A.; Fabian, U. A.; Fasanmade, A. A.; Olaniyi, J. A.; Oyewole, O. E.; Owolabi, M. O.|; Adebusuyi, J. R.; Hassan, O.|; Ajobo, B. M.; Ebesunun, M. O.; Adigun, K.; Akinlade, K. S.; Okoli, S. U.; Arinola, O. G.; Agbedana, E. O.
Reproductive dysfunction is associated with metabolic syndrome. Since fertility is highly valued in Africa, preliminary data on the association of metabolic syndrome with indices of reproductive function in premenopausal Nigerian women was provided. Sixty six premenopausal participants (44 with metabolic syndrome and 22 controls) aged 18-45 years were purposely selected for this study. Reproductive history, blood pressure and waist circumference were obtained by standard methods. Fasting blood was obtained for pituitary hormones, adipokines, sex hormone and sex hormone binding globulin, and inhibin B assays by EIA, ELISA and electro-chemiluminiscence. Plasma glucose, triglycerides and high density lipoprotein cholesterol were estimated by enzymatic methods. Free androgen index and oestrogen-testosterone ratio were calculated. Data obtained were statistically significant at P<0.05. All reproductive factors except follicle stimulating hormone and free androgen index levels were similar in both groups (P>0.05). Leptin levels were higher while adiponectin levels were lower in MS group than controls (P<0.05). Reproductive function appears sustained in MS. However, altered adipokines may relate to MS.