Browsing by Author "Oniya, M. O."

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    IgG Enzyme-linked Immunosorbent Assay (ELISA) for immunodiagnosis of Schistosoma haematobium infected subjects living in an endemic Nigerian village
    (Academic Journals, 2011-04) Oniya, M. O.; Omosun, Y. O.; Anumudu, C. I.; Nwuba, R. I.; Odaibo, A. B.
    Schistosoma haematobium infects school children in areas where the disease is endemic. This study was carried out to determine the reliability of IgG Enzyme-Linked Immunosorbent Assay (ELISA) as an immunodiagnostic tool, using whole antigen derived from Schistosoma eggs. Children of school going age living in Ipogun village, in south western Nigeria; a schistosomiasis endemic community; were used in this study. The sensitivity of the IgG ELISA in detecting positive cases was 68.0% and there was a significant correlation (P < 0.05) between IgG titres and the intensity of infection. However, the specificity was 59.3%, slightly lower than the sensitivity. Results from this study shows that IgG ELISA can be used as a diagnostic tool for determining S. haematobium infection, as it provides an evidence for the intensity of the infection in the infected individuals.
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    Schistosome specific antibodies in individuals co-infected with malaria in Southwest Nigeria
    (Nigerian Society of Parasitology, 2012-09) Anumudu, C. I.; Alabi, O.; Oniya, M. O.
    A cross-sectional study design in two primary schools in Ibadan and Akure was used to determine the prevalence of urinary schistosomiasis, and the human humoral immune response to schistosome antigens in individuals with malaria co-infection. Urine samples were collected from 163 children, while 112 gave blood samples. Malaria parasitaemia was determined by microscopy after Giernsa staining and schistosomiasis by centrifugation technique. Serum samples were analyzed for antibodies to crude S. mansoni soluble egg, adult worm antigens, and crude S. haematobium egg antigen by ELISA. The sample population consisted of 40% (62/163) infected with schistosomiasis, 31% (50/163) with malaria, and 6% (10/163) co-infected. All the co-infected students had asymptomatic malaria with parasite densities ranging from 200 - 4,420 parasites/ul blood. IgG titres to the various Schistosoma antigens did not vary significantly. However, antibody titres to the soluble egg antigen increased with age of volunteers. Antigen specific isotype distribution showed a higher prevalence of IgG3 and lgG4.