Browsing by Author "Oridupa, O. A."

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Anti-inflammatory and analgesic effects of methanol extract of stellaria media (L.) Vill leaf
(2012-01) Oyebanji, B. O.; Saba, A. B.; Oridupa, O. A.
The anti-inflammatory and analgesic effect of the methanolic extract of Stellaria media (L.) Vill leaf was studied using albumen-induced paw oedema and formalin-induced paw lick in rats as the anti-inflammatory test models; acetic acid-induced writhing, hot plate and tail flick tests in mice as the analgesic models. Three groups of five rats or mice each were administered orally with the leaf extract of S. media at 100mg/kg, 300mg/kg or 500mg/kg body weight respectively. A fourth group was administered with Indomethacin (5mg/kg b.w) and distilled water (10mls/kg b.w) for the control group. The extract of S. media dose-dependently, significantly (p<0.05) inhibited egg albumen-induced paw oedema as effectively as Indomethacin. The late phase of the formalin response was also inhibited. The extract at 300mg/kg body weight produced a significant (p<0.05) inhibition of the acetic acid-induced abdominal constrictions in mice compared to the control group and mice administered with indomethacin. The analgesic property of the extract was also exhibited in the tail flick test as the extract significantly (p<0.05) increased the tolerance of the mice to pain relative to indomethacin-treated mice. The methanolic extract of S. media showed potent peripherally and centrally mediated anti-inflammatory and analgesic properties. The analgesic effect appears mediated through inhibition of release of histamine, serotonin and kinins, prostaglandin, cyclooxygenase and slow reacting substance.
Design of cissus–alginate microbeads revealing mucoprotection properties in anti-inflammatory therapy
(Elsevier B.V., 2015) Okunlola, A.; Odeku, O. A.; Lamprech, A.; Oyagbemi, A. A.; Oridupa, O. A.; Aina, O. O.
Cissus gum has been employed as polymer with sodium alginate in the formulation of diclofenac microbeads and the in vivo mucoprotective properties of the polymer in anti-inflammatory therapy assessed in rats with carrageenan-induced paw edema in comparison to diclofenac powder and commercial diclofenac tablet. A full 23 factorial experimental design has been used to investigate the influence of concentration of cissus gum (X1); concentration of calcium acetate (X2) and stirring speed (X3) on properties of the microbeads. Optimized small discrete microbeads with size of 1.22 ± 0.10 mm, entrapment efficiency of 84.6% and t80 of 15.2 ± 3.5 h were obtained at ratio of cissus gum:alginate (1:1), low concentration of calcium acetate (5% w/v) and high stirring speed (400 rpm). In vivo studies showed that the ranking of percent inhibition of inflammation after 3 h was diclofenac powder > commercial tablet = cissus > alginate. Histological damage score and parietal cell density were lower while crypt depthand mucosal width were significantly higher (p < 0.05) in the groups administered with the diclofenac microbeads than those administered with diclofenac powder and commercial tablet, suggesting the mucoprotective property of the gum. Thus, cissus gum could be suitable as polymer in the formulation of non-steroidal anti-inflammatory drugs ensuring sustained release while reducing gastric side effects.