Browsing by Author "Osungunna, M. O."

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Bacteriological and antibiotic sensitivity patterns of bacterial Isolates from creams and lotions hawked in Sagamu, Ogun State
(Asian Network for Scientific Information, 2010) Osungunna, M. O.; Oluremi, B. B.; Adetuyi, A.
Fifteen cosmetic products, consisting of 10 creams and 5 lotions were randomly purchased from a local market in sagamu and their microbial qualities studied in addition to the antibiotic sensitivity patterns of different isolates obtained from the selected creams and lotions. While only one of the creams was devoid of any microorganism including fungi, organisms isolated from others include Staphylococcus aureus 38%; Klebsiella, 28%; Pseudomonas aeruginosa, 21%; Bacillus spp, 7% and Penicillium, 28%. Antibiotic sensitivity study reveals that these isolates displayed different sensitivity patterns to the antibiotics used but cotrimoxazole, tetracycline and nalidixic acid will be of assistance in case of infection from these cosmetic products. However, majority of the creams and lotions evaluated did not meet the official monograph’s requirements and as such may be a potential health hazard to unsuspecting consumers moreso that all the isolates display some degree of resistance to various antibiotics used.
Comparative assessment of antibacterial activity of uvaria chamae parts
(Academic Journals, 2010) Oluremi, B. B.; Osungunna, M. O.; Omafuma, O. O.
Methanolic extracts of the root, stem and leaf of Uvaria chamae were evaluated for their antibacterial activity against Methicillin-Resistant Staphylococcus aureus (MRSA), Staphylococcus aureus, Escherichia coli, Klebsiella spp, Proteus spp, and typed strains E. coli ATCC 25922, S. aureus ATCC 25923 and Pseudomonas aeruginosa ATCC27853 using standard agar diffusion method at 50, 100, 150, 200 and 250 mg/ml. Preliminary phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, cardiac glycosides and cyanogenic glycosides. In general, the stem bark extract inhibited the growth of all the tested organisms with leaf extract showing the least antibacterial activity. The MIC of methanolic stem extract on E. coli, MRSA, Klebsiella spp, Proteus spp, E. coli ATCC 25922, S. aureus ATCC 25923 and P. aeruginosa ATCC 27853 were 5.62, 12.59, 200, 35.48, 28.18, 5.62 and 19.95 mg/ml respectively while that of root extract with the exclusion of Klebsiella spp were 31.62, 10.0, 3.55 2.82, 12.59 and 39.81 mg/ml respectively. Methanolic leaf extract was found to have MIC 200, 19.95, 250, 250, 250 and 200 mg/ml respectively with no MIC value against P. aeruginosa.
Comparative microbial quality of jedi drinks sold in two major cities in Nigeria
(Asian Network for Scientific Information, 2010) Osungunna, M. O.; Oluremi, B. B.; Talabi, I.
The microbial quality of ten samples of Jedi herbal preparations sold for the treatment of anal fistula in major motor parks of Sagamu and Ibadan, both in Nigeria was studied and compared. Phytochemical analysis of the samples revealed that all the samples contained Saponins and Tannins. Alkaloids and cardenolides were also present to varying extent. These secondary metabolites are known to be responsible for the prevention of anal fistula. 100% contained Pseudomonas aeruginosa, 80% from Sagamu contained Klebsiella spp; 60% from Ibadan contained Bacillus spp, 30% of the samples contained Staphylococcus aureus and 70% contained fungi. Antibiotic sensitivity patterns revealed that the Gram-negative isolates were most sensitive to ofloxacin, a quinolone antibiotic while the Gram-positive isolates were most sensitive to Streptomycin, an aminoglycoside
Evaluation of anticaries activity of selected mouthwash marketed in Nigeria
(Pharmacotherapy Group,, 2010) Oluremi, B. B.; Osungunna, M. O.; Idowu, O. A.; Adebolu, O. O.
Purpose: This work was aimed at evaluating four brands of mouthwash marketed in Nigeria for their anticaries activity. Method: The susceptibility pattern of bacterial isolates obtained from 30 patients having carious teeth to the selected mouthwash was determined using the cup-plate method. The zones of inhibition produced by the mouthwash against the bacterial isolates were measured to determine the degree of susceptibility. Results: The organisms isolated from the patients were Staphylococccus aureus, Klebsiella sp, Streptococcus sp, Escherichia coli and Pseudomonas aeruginosa in the following proportions: 30, 27, 20, 17 and 7 %, respectively. Anticaries evaluation revealed that 0.047 % thymol in ethanol displayed the highest degree of anticaries activity against all the Streptococcal species tested. Incorporation of preservatives in mouthwashes may adversely affect their anticaries activity as indicated by the fact that the preserved mouthwash exhibited lower activity than the unpreserved mouthwash. Conclusion: Anticaries activity is optimally achieved by using appropriate mouthwash combinations as this prevents both the initiation and progression of dental caries. The use of preservatives in mouthwash formulations should be done with caution as this may interfere with mouthwash activity.
Phytochemical and antimicrobial screening of the leaf extract of diospyros barteri. Gurke.
(2010-08) Oluremi, B. B.; Osungunna, M. O.; Ogbole, O. O.
Diospyrous bateri Gurke family Ebenaceae, has been commonly used by traditional medical practitioners as anti-nfectious agent. In this study, preliminary screening of phytochemical constituents of D. barteri was carried out in addition to evaluating the antimicrobial activity of four different extracts of this leaf on some common bacteria species. The antimicrobial activity of methanol, chloroform, hexane and aqueous extracts of the leaves were tested using the agar diffusion method on two Gram positive, three Gram negative bacteria viz; Staphylococcus aureus, Proteus vulgaris, Klebsiella pneumoniae Escherichia coli, and Pseudomonas aeruginosa, The preliminary phytochemical screening revealed the presence of alkaloids, saponins and tannins while other secondary metabolites were absent. The study amply demonstrated the broad-spectrum antimicrobial property of the polar extracts while the non polar extract exhibits no activity. The minimum inhibitory concentration of the methanol extract on Proteus vulgaris, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Staphylococcus aureus were 1.20mg/ml, 2.24mg/ml, 3.55mg/ml, 9.12mg/ml and 10.0mg/ml respectively, while the minimum inhibitory concentration of the water extract on Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus were 8.91mg/ml, 9.55mg/ml, 10.59mg/ml, 10.96mg/ml and 71.78mg/ml respectively.