Browsing by Author "Oyinloye, O. E."

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GC-MS profiling and evaluation of antioxidant and antimicrobial properties of methanolic extract and fractions of the leaves of solanum dasyphyllum schumach and thonn
(West African Postgraduate College of Pharmacists, 2023) Oyinloye, O. E.; Alabi, O. S.; Ademowo, O. G.
Background: Solanum dasyphyllum is a plant with several ethno-medicinal uses and to date, there is little scientific validation of its folkloric claims compared to other species of the Solanaceae family. Objective: This study determined the antimicrobial and antioxidant potential of methanol extract and fractions of the leaves of S. dasyphyllum and identified the phyto-constituents using GC-MS technique. Methods: Crude methanolic extract of S. dasyphyllum leaves were subjected to Phytochemical screening and liquid-liquid fractionation. Crude extracts and fractions were subjected to antimicrobial screening, including minimum inhibitory concentrations (MICs) and minimum bactericidal/fungicidal concentrations (MBC/MFC) determination, time-kill kinetics study, antioxidant activity and Gas Chromatography-Mass Spectrometry (GCMS) analysis. Results: Phytochemicals present included flavonoids, alkaloids, saponins, cyanogenic glycosides, tannins and reducing sugars. Antimicrobial activities were recorded against all the isolates (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Aspergillus niger, Rhizopus spp., Candida albicans and Penicillium spp.) with MIC between 0.25 and >4 mg/mL, MBC of 1 and >4 mg/mL and MFC of 0.5 and >4 mg/mL. Time-kill kinetics study showed dichloromethane and ethylacetate fractions to be bactericidal. Crude extract displayed moderate antioxidant (IC50 = 425.51μg/mL) and GC-MS analysis showed 29 metabolites, including phenols and polyphenols derivatives, benzofuranone derivative and esters of decanoic acid derivatives, all of which are known to have anti-infective and anti-oxidant properties. Conclusion: This study therefore elucidates the potentials of the Solanum dasyphyllum plant as a good source of bioactive compounds including those with anti-infective and antioxidant properties.
Preliminary phytochemical screening, antimicrobial potentials and GC-MS analysis of Secamone afzelli Rhoem (asclepiadaceae) leaves extracts
(Faculty of Physical Sciences and Faculty of Life Sciences, Univ. of Ilorin, Nigeria, 2020) Oyinloye, O. E.; Alabi, O. S.
Secamone afzelli is used ethno-medicinally as remedies for stomach, colic and gastro-intestinal tract infections. This study screened the hexane, ethyl acetate and ethanol extracts of S. afzelli leaves for phytocompounds, antimicrobial activity and identified bioactive compounds in the most active extract using Gas Chromatography Mass Spectrometry (GC-MS) analysis. S. afzelli (200mg) leave was extracted by soxhlet apparatus using three solvents (hexane, ethyl acetate and ethanol) and the phytochemicals determined. Antimicrobial susceptibility and minimum inhibitory concentrations (MICs) was determined by agar-diffusion and agar-dilution methods respectively against selected resistant bacterial and fungal clinical isolates. The most active of the three crude extracts was analysed by GC-MS for bioactive compounds identification. Presence of carbohydrates, anthraquinone, glycosides, proteins, tannin, phenolic compounds and steroids were determined. The three extracts (hexane, ethyl acetate, and ethanol extracts) had antibacterial (Range of inhibition zone = 10 to 18 mm and MICs 12.5 to 200 mg/mL) but no antifungal activity. The ethanol extract was the most active with 29 bioactive compounds including piperazine, phytol, leucine methyl ester, N-methyl-N-(1-methylethyl)- 1Pentanamine, 7-propyl-pyrrolizin-1-thione and 4,5-dimethylnonane. The antibacterial activity and arrays of bioactive compounds detected in S. afzelli leaves suggest that the plant may be a good source of novel antimicrobial compounds.