Browsing by Author "Somade, O.T."

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"Combined use of earthworm (Alma millsoni) and bacterium (Bacillus sp.) improved the bioremediation of spent engine oil contaminated soil"
(Taylor & Franciis, 2018) Adeyi, A.O.; Nneji, L.M.; Adeyi, E.O.; Somade, O.T.; Agbaogun, B.K.
This study was carried out to assess the effectiveness of the combined use of earthworm (Alma millsoni) and bacterium (Bacillus sp.) in the bioremediation of spent engine oil (SEO) contaminated soils. A. millisoni were collected from the Botanical Garden of University of Ibadan, Nigeria. The stock culture of hydrocarbonoclastic Bacillus sp. was used for the bioremediation study. A set-up of eight pots containing 1000 g soil sample and 20 g of cow dung were mixed with 100, 75, 50 and 0 mL SEO respectively. Each of the set up was subjected to bioremediation agents; A. millisoni alone, Bacillus sp alone, A. millisoni and Bacillus sp, no treatment (control) in duplicate. Treatment of 100 ml SEO contaminated soil with combined A. millisoni and Bacillus sp resulted in significantly (P < 0.05) lower levels of TPH and heavy metals (Ni and Pb), higher microbial activity and C: N ratio than the other treatments. The effectiveness of the bioremediating agents as indicated by reduction in the indices of petroleum hydrocarbon pollution followed the pattern: vermibacterium > bacterium > vermi-remediating agents. Earthworms exposed to 100mLSEO-contaminated soil had higher CAT, SOD, and GPx activities compared to the control. Findings indicated that A. millisoni with Bacillus sp. can synergistically improve bioremediation of SEO contaminated soils.
"Earthworm-assisted bioremediation of petroleum hydrocarbon–contaminated soils from motorcar mechanic workshops in Ibadan, Oyo State, southwestern Nigeria"
(Taylor & Francis, 2016) Nneji, L.M.; Somade, O.T.; Adeyi, A.O.
Enhanced microbial bioremediation of petroleum hydrocarbon–contaminated (PHC) soils with the earthworm Alma millisoni and the bacterium Bacillus spp. was conducted. The petroleum-contaminated topsoils (PCTS) (0–15 cm) collected from motorcar mechanic workshops were thoroughly mixed, sieved, and air dried for 7 days. The pH, water holding capacity (WHC), total nitrogen (N), organic carbon (OC), heavy metal (HM), and bacteriological analysis of the soil samples were evaluated. The indigenous bacterial isolates were subjected to 1%, 5%, and 50% of spent engine oil (SEO), incubated for 7 days at 37_C, and the isolate with the highest tolerance pattern was used for the remediation. Out of four indigenous bacteria isolated, Bacillus spp. had the highest tolerance to SEO. Preliminary exposure assessments of A. millisoni to PHC soils (100%, 60%, 50%, and 40% PHC) were carried out using 48-h avoidance response, coiling exhibition, swollen clitelium, 14-day survival tests, and antioxidant enzyme activities such as catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), and glutathione peroxidase (GPx). Subsequently, four treatments of 1 kg soil mixed with 100%, 75%, 50%, and 0% PCTS were designed and spiked with 20 g of dried cow dung. Each of the treatments consisted of four setups, viz., A. millisoni alone, A. millisoni and Bacillus spp., Bacillus spp. alone, and control. The bacterial counts, total petroleum hydrocarbon (TPH), total and bioavailable HM, and total OC and N of the soils were evaluated every 7 days for 35 days. Significant increases in the activities of CAT, SOD, GPx, and GST compared with control were recorded in A. millisoni exposed to the various treatments. Treatment with combined A. millisoni and Bacillus spp. resulted in significant (p < .05) reduction in TPH, reduction in total and bioavailable heavy metals, and increased total OC and N of the soil compared with other treatments. The percentage reduction in TPH and heavy metals with concomitant increase in total OC and total N recorded in the 50% PHC soils followed the order A. millisoni and Bacillus spp. > A. millisoni alone > Bacillus spp. alone.Hence, enhanced bioremediation using A. millisoni and Bacillus spp. may be a good biocatalyst in the remediation of petroleum hydrocarbon–contaminated soils.
Ferulic acid mitigates 2-methoxyethanol-induced testicular oxidative stress via combined downregulation of FoxO1, PTEN, and modulation of Nrf2-Hmox1-NQO1 signaling pathway in rats
(Elsevier, 2023) Adeyi, O.E.; Somade, O.T.; James, A.S.; Adeyi, A.O.; Ogbonna-Eze, S.N.; Salako, O.Q; Makinde, T.V.; Ajadi, O.M.; Nosiru, S.A.
INTRODUCTION: Ferulic acid (FERA) is a natural antioxidant that is richly found in herbs, including Ligusticum chuangxiong, Cimicifuga heracleifolia, and female ginseng (Angelica sinensis), which are utilized in modern Chinese medicine, and in cereals/grains including rice, which is mostly consumed by humans. 2METE on the other hand, is a ubiquitous substance that has many industrial applications, including use in the preparation of dyes for textiles, hydraulic fluid for automobiles, paints, and liquid soaps. It is a testicular toxin, which can induce oxidative stress in the testis of rats. Therefore, this study investigated the effect of FERA, which was concomitantly administered, against 2-methoxyethanol (2METE)-induced testicular oxidative stress in rats. METHODS: Male Wistar rats totaling twenty (20), separated into four (4) groups, were used for the study. Rats in group one served as the control, rats in groups two and three were administered 100 mg/kg of 2METE only for 30 consecutive days, but only rats in group three were concomitantly treated with 50 mg/kg of FERA for the same duration, while rats in group four were treated with 50 mg/kg of FERA only. RESULTS: Following analysis, 2METE administration caused a significant reduction in the relative testes weight (RTW), NAD(P)H quinone oxidoreductase 1 (NQO1), and reduced glutathione (GSH) levels, as well as superoxide dismutase (SOD) and glutathione S-transferase (GST) activities in the testis of rats compared with the control. Moreover, 2METE administration also significantly increased the testicular levels of malondialdehyde (MDA), nitric oxide (NO), and RNA gene expressions of heme oxygenase 1 (Hmox1), nuclear factor erythroid 2-related factor 2 (Nrf2), forkhead box protein O1 (FoxO1), and phosphatase and tensin homolog deleted on chromosome 10 (PTEN) compared with the control. FERA treatment, on the other hand, significantly decreased the testicular levels of MDA, as well as Nrf2, Hmox1, PTEN, and FoxO1 gene expressions, and significantly increased the testic- ular GSH and NQO1 levels, activities of GST, SOD, glutathione peroxidase (GPx), and catalase (CAT) compared with 2METE only administered rats. CONCLUSION: 2METE-induced testicular oxidative stress, marked by the depletion of the endogenous antioxidant systems, was recorded, which resulted in the activation of PTEN, FoxO1, and Nrf2 genes in rats. FERA demon- strated a strong antioxidant effect by restoring the levels and activities of the endogenous antioxidants as well as downregulating the expressions of PTEN, FoxO1, and Nrf2 in the testis of rats.
"Launaea taraxacifolia leaf partitions ameliorate alloxan-induced pathophysiological complications via antioxidant mechanisms in diabetic rats"
(Elsevier, 2020) Gbadamosi, I.T.; Adeyi, A.O.; Oyekanmi, O.O.; Somade, O.T.
Launaea taraxacifolia (Wild.) Amin ex. Jeffery belongs to family Asteracaea. The plant is used for treatment of diseases and eaten as vegetable in Nigeria. This study investigated the ameliorative potentials of L. taraxacifolia leaf partitions in alloxan induced diabetic complications. Male Albino rats were divided into eleven groups of five rats each. Diabetes was induced following intraperitoneal administration of 150 mg/kg alloxan monohydrate and was treated with 200 and 300 mg/kg of each partitioned fractions. Hyperglycemia was reversed in all treated rats within seven days of treatments. Rats treated with the partitions showed significant increase in hematological parameters compared with diabetic control. Nhexane fraction had the best overall effect against oxidative stress particularly on heart and pancreas reduced glutathione (GSH), superoxide dismutase (SOD) and kidney glutathione S-transferase (GST) activities. The various degrees of degeneration observed in the kidney, liver, pancreas and heart of the untreated diabetic rats were milder in rats treated with partitions. The results therefore revealed the ameliorative potentials of the partitioned fractions of L. taraxacifolia leaf extract against diabetes mellitus complications via activation of the antioxidant enzymes.
Syringic acid demonstrates better anti-apoptotic, anti-inflammatory and antioxidative effects than ascorbic acid via maintenance of the endogenous antioxidants and downregulation of pro-inflammatory and apoptotic markers in DMN-induced hepatotoxicity in rats
(Elsevier, 2023) Adeyi, O.E.; Somade, O.T.; Ajayi, B.O.; James, A.S.; Adeyi, A.O.; Olayemi, Z.M.; Tella, N.B.
Dimethyl nitrosamine (DMN) is a known hepatotoxin, carcinogen, and mutagen. This study is therefore carried out to investigate the therapeutic effects of syringic acid (SYRA) and ascorbic acid (ASCA) in DMN-induced hepatic injury in rats. Following DMN administrations, malondialdehyde (MDA), nitric oxide (NO) and reduced glutathione (GSH) as well as activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD) were significantly increased. Also significantly increased were levels of tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Following treatment with SYRA and ASCA, the activities of ALT, AST, GPx, CAT and SOD, as well as MDA, GSH, TNF-α, IL-1β, and NFkB levels were significantly reduced. Overall, both treatments were effective, but SYRA had a better therapeutic effect than ASCA. Therefore, this promising potential of SYRA can be taken advantage of in the treatment of DMN-induced hepatic injury.