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    A new spectrophotometric method for the determination of gabapentin usingchromotropic acid
    (Istanbul Medipol University, 2018) Adegbolagun, O. M.; Thomas, O. E.; Aiyenale, E. O.; Adegoke, O.A.
    The purpose was to develop a colorimetric method for determining gabapentin. The method was based on the diazo coupling reaction between diazotized gabapentin and chromotropic acid. The method was validated using ICH guidelines before its application to generic brands of gabapentin. Coupling reaction generated an orange azo adduct whose absorbance was linearly correlated with concentration in the range of 1-6 μg/mL at 470 nm. The method was accurate and precise with recovery range of 97.6-103.1%; intra- and inter-day precisions (%RSD) were less than 0.65% and showed no statistical difference when compared with reference method in the analysis of the dosage forms. The 3D optimization of the adduct revealed an E-type configuration around the azo linkage which would contribute to its stability. The new method can serve as a reliable alternative to the official method for the routine analysis of gabapentin in bulk and dosage forms.
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    New spectrophotometric method for the determination of Gabapentin in bulk and dosage forms using p-dimethylaminobenzaldehyde
    (Taylor & Francis, 2018) Adegoke, O. A.; Adegbolagun, O. M.; Aiyenale, E.; Thomas, O.E.
    A new simple, accurate and economic spectrophotometric method based on azo dye derivatization for the determination of gabapentin (GBP) was developed. Critical factors were optimized. The method was validated and assay of dosage forms was done. Spot tests and TLC confirmed the formation of azo adduct. A 0.3M NaNO2 solution using 2M HCl was used for diazotization. The optimal temperature and time were 30°C and 10 min. Azo adducts were determined at 430 nm. Methanol was found to be the best solvent. Gabapentin coupled at a ratio of 1:1 with DMAB. The assays of GBP were linear over the range 1–6 μg/mL (r = 0.9973) and LOD of 0.8322 μg/mL. The methods were accurate (error < 2%) and precise (RSD < 0.5%). The methods were successfully applied to the assay of GBP in dosage forms and compared favorably with reference method (p > .05). The successful diazotization of gabapentin and the azo adduct formation with DMAB is reported for the first time.