Medical Microbiology & Parasitology
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Item Serological profile of convalescent COVID-19 patients at an infectious diseases hospital in Nigeria(Sciencedomain International, 2021) Fowotade, A.; Fasuyi, T. O.; Omoruyi,E. C.; Alonge, T. O.Background: IgG antibodies may serve as promising targets to detect and evaluate immune responses against the SARS-CoV-2 virus. Both IgA and IgM antibodies target the spike protein’s receptor binding domain and are rapidly decayed, while IgG antibodies remain relatively stable for longer periods in COVID-19 patients. Objectives: The current study was designed to detect the presence of SARS-CoV-2 antibodies among convalescent COVID-19 patients and to evaluate the relationship between these antibodies, the symptom grade and their baseline Cycle Threshold (CT) by RT-PCR. Methods: Eighty-nine convalescent COVID-19 patients on admission were recruited and tested until negative by RT-PCR. Sera obtained from participants were screened for SARS-CoV-2 IgM and IgG antibodies using rapid lateral flow assays. Results: It was observed that 93,3% and 77,5% respectively had IgM and IgG antibodies against the S1 protein of SARS-CoV-2. Majority (74,0%) presented with mild COVID-19 symptoms with a mean RT-PCR Ct value of 31,4. Conclusion: Convalescent COVID-19 patients develop a fairly good level of IgG antibodies. Theantibody status is not dependent on CT value or symptom grade. However, there was a significant correlation between baseline CT and time taken to test negative by RT-PCR.Item Re-emergence of Monkeypox in Nigeria: a cause for concern and public enlightenment(African Society for Clinical and Experimental Microbiology, 2018) Fowotade, A.; Fasuyi, T. O.; Bakare, R. A.Monkey pox infection is a zoonotic infection transmitted by direct or indirect contact with blood, body fluids and lesions of an infected animal. Human to human spread of Monkey pox has been described and infection is usually self-limiting, with an incubation period of 6-16 days. In Nigeria, the last case of monkey pox infection was recorded over 46 years ago. The recent emergence in Nigeria occurred in the year 2017 and was reported to have spread to 24 states with 228 suspected individuals affected. Laboratory diagnosis, as well as management and prevention of monkey pox infection in Nigeria, remain challenging as Nigeria is a resource-poor country with limited infrastructure, technical skill and training which is required in making a diagnosis. The ability of the monkey pox virus to evolve, its potential bioterrorism potential, as well as its recent emergence in Nigeria further justifies the need for improving the understanding of the presentation and prevention of monkey pox infection.Item Internal and external quality control in the medical microbiology laboratory(African Society for Clinical and Experimental Microbiology, 2018) Fowotade, A.; Fayemiwo, S. A.; Bongomin, F.; Fasuyi, T. O.; Aigbovo, O. A.; Adegboro, B.Culture media play a very important role in bacteriology as they are used in the isolation, identification and antimicrobial susceptibility testing. It is essential that the quality of media be safeguarded to have a successful microbiology laboratory. Microorganisms usually show typical morphological appearance and properties on solid media. Variations in the composition of the medium may alter this appearance and properties. There is therefore a need to ensure good quality media, which is capable of giving satisfactory results by ensuring a proper quality management system. Often times, majority of laboratories prepare their media for routine diagnostics and research purposes. Therefore, it is essential that certain parameters of media are checked thoroughly before they are considered suitable for laboratory use. Control methods are discussed in details in this report.