Chemical pathology
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Item Endocrine disruptors-arsenic, cadmium and lead in pre and postmenopausal black women with breast cancer(University College Hospital, Ibadan, 2017) Ajayi, O. O.; Charles-Davies, M. A.; Anetor, J. I.; Ademola, A. F.Background: The involvement of toxic metals in adiposity has been suggested to be contributory to the high incidence of breast cancer, particularly in sub-Saharan Africa. This study is aimed at evaluating serum arsenic, cadmium and lead in relation to adiposity and blood pressure in Nigerian women with breast cancer. Methodology: The study comprised 85 women newly diagnosed with breast cancer pre-therapy (cases) matched with 84 apparently healthy women without breast cancer (controls) according to age and menstrual phase. Arsenic (As), cadmium (Cd) and Lead (Pb) levels were determined by atomic absorption spectrophotometry. Blood pressure and anthropometry were determined by standard methods. Data analysed by Student’s t-test and Pearson correlation coefficient were considered statistically significant at p<0.05. Results: Cd and Pb levels were significantly higher in cases, compared with controls (p<0.05). Waist circumference (WC), hip circumference (HC), weight, height, waist hip ratio (WHR), waist height ratio (WHtR) were significantly higher in cases compared with controls (p<0.05). Cadmium positively correlated with diastolic blood pressure while FT4 inversely correlated with arsenic in the cases (p<0.05). Conclusion: Observations in this study suggest the involvement of these toxic metals in adiposity which could be involved in breast carcinogenesis.Item Serum polychlorinated biphenyls and bisphenol-A levels in Nigerian women with breast cancer(2014-06) Ajayi, O.; Charles-Davies, M. A.; Anetor, J. I.; Ademola, A. F.Endocrine disruptors (EDs)-polychlorinated biphenyls (PCBs) and bisphenol-A (BPA) have been associated with increased risk of breast cancer (B.Ca). In Nigeria, there is increase in breast cancer incidence. This has been attributed to increased use and exposure to EDs. Although, several studies have been done on breast cancer, there is paucity of information on the serum levels of these EDs in Nigerian women. This study therefore aims at determining the serum levels of PCBs and BPA in breast cancer patients.Eighty women aged 18-70 years were recruited with informed consent after institutional ethical approval. They were 40 participants with breast cancer pre-treatment (cases) matched with 40 apparently healthy women (controls). They were recruited from a tertiary hospital in Ibadan, Nigeria. Demographic indices: age, age at menarche (AM), age at menopause (AMP) and anthropometric indices: height, weight, body mass index (BMI), waist circumference (WC), hip circumference (HC), waist-hip ratio (WHR) and waist-height ratio (WHT) were obtained from the pre-test questionnaire administered and standard methods respectively.10 ml of venous blood was collected from each participant and sera obtained were analyzed for PCB and BPA. These were determined by High performance liquid chromatography (HPLC). Data were analyzed using Student t-test and Pearson correlation coefficient, p was significant at <0.05.Results showed increases in the height, weight, WC, HC, WHR, WHT, PCB and BPA serum levels in cases compared with controls. Positive correlations were observed among WC and WHR, WHT, HC; HC and WHT; WHR and WHT in both cases and controls. However, positive correlations among height, weight, WC and WHR; BMI and weight were observed in cases only. Weight correlated negatively with age at menarche (AM) only in controls. The differences and correlations were statistically significant (p<0.05).Mechanisms involving PCB, BPA and adiposity may be involved in breast cancer aetiology. Reduction of environmental pollution and dietary modulation may be helpful.Item Oestrogen levels and humoral immune parameters in Nigerian breast cancer patients(2009-09) Etuk, A. I.; Charles-Davies, M. A .; Arinola, O. G.Objectives: Endocrine and immune interactions mediate breast cancer which is currently incurable. This study attempts at elucidating mechanisms by which breast cancer progresses by determining the levels of oestradiol and humoral immune parameters at different stages of breast cancer compared with women without breast cancer. Patients and Methods: 59 subjects (29 breast cancer attending Surgery Clinics of 2 teaching hospitals in Lagos State, Nigeria and 30 controls) aged 23-82 years were recruited using convenience sampling. Serum was obtained from 10ml of fasting blood from each subject and stored frozen at -20“C until analysis. Oestradiol and albumin were estimated using commercial kits (Adaltis Italia S.P.A and Biolab SA, Maizy, France) respectively. Haptoglobin, alpha-2- macroglobulin, caeruloplasmin, IgG, IgA, IgM were analysed by single radial iinmuno-diffusion method. SPSS version 10 was used for statistical analysis. Main Results: Oestradiol, haptoglobin and caeruloplasmin were significantly increased in premenopausal breast cancer patients compared with premenopausal controls, while only oestradiol was significantly increased in postmenopausal breast cancer patients compared with postmenopausal controls. Moreover, IgM, haptoglobin and caeruloplasmin were significantly reduced in stage III breast cancer patients compared with stages II and IV breast cancer patients. Conclusions: Although inflammation is common to all stages of breast cancer, immunosuppression is most pronounced in breast cancer patients in stage III of the disease.Item Micronutrient levels in the plasma of Nigerian females with breast cancer(Academic Journals, 2008-06) Arinola, O. G.; Charles-Davies, M. A.Background: Breast milk is important for the overall well-being of infants. Although lactation is relatively robust in the face of poor nutrition, the implication of poor nutrition on non-nutritive factors in breast milk is inconclusive. Objective: This study was designed to find associations between nutritional and immune factors in maternal blood and breast milk with the aim to improve the needed public and individual strategies for a healthy infant. Method: A cross sectional study was conducted on 61 lactating Nigerian women aged 23-40years within the first 3 months postpartum. Anthropometric measurements were obtained while nutritional factors (total protein, albumin) and immunoglobulin classes (IgG, A and M) were estimated by Biuret, Bromocresol green and single radial immunodiffusion methods respectively in maternal plasma and breast milk. Results: Most (73.5%) of the lactating mothers had normal mean body mass index (i.e. not under weight nor obese) and the mean levels of plasma total protein, albumin, IgG, IgA and IgM were within normal reference ranges in these mothers. Nutritional and immunological indices increase in the plasma with length of lactation but decrease in breast milk with lactation. There were no correlation between BMI, plasma indices and milk indices in these lactating mothers. Conclusion: This study supports the superiority of colostrum over transitional or matured milk for the protection and nourishment of infants.