FACULTY OF BASIC MEDICAL SCIENCES
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Item Cytokine profile in Nigerians with tubal infertility(Termedia Publishing House Ltd, 2016) Nsonwu-Anyanwu, A. C.; Charles-Davies, M. A.; Taiwo, V. O.; Bello, F. A.; Bin, L.; Oni, A. A.Background: Immune response to genital Chlamydia trachomatis infection is involved in both immunity and pathology. The cytokine profile during infection has been implicated in the disease outcome, either resolution or severe sequelae. Serum cytokines of Chlamydia positive Nigerian women with tubal infertility were assessed to determine their possible relationship with tubal occlusion. Material and methods: One hundred and fifty age-matched consenting women (100 fertile and 50 with tubal infertility) were recruited based on C. trachomatis antibody positivity and grouped into infertile Chlamydia positive (CTpos) women (n = 50), fertile Chlamydia positive women (n = 50) and fertile Chlamydia negative (CTneg) women as controls (n = 50). High vaginal swabs and endo-cervical swabs were collected for microscopy, culture and gram staining. Cytokines [transforming growth factor β1 (TG F-β1), interferon γ (IFN-γ), tumor necrosis factor α (TNF-α), interleukin (IL )-4, IL -10 and IL-17A] were estimated by ELISA in sera. Data were analyzed using ANOVA, χ2 and Spearman’s correlation at p = 0.05. Results: Lower IFN-γ levels were observed in infertile women compared to fertile women. Fertile CTneg women had significantly higher TNF-α, and TGF-β1 compared to fertile and infertile CTpos women, respectively. Lower IL-10 levels were seen in fertile CTpos women compared to the infertile CTpos group. Vaginal discharge was negatively correlated with TNF-α and IFN-γ and positively with IL-4 in Chlamydia positive women. Conclusions: Chlamydia positive women with tubal infertility have higher IL -10 and lower IFN-γ levels than controls, which may contribute to their development of tubal pathology.Item Female reproductive hormones and biomarkers of oxidative stress in genital chlamydia infection in tubal factor infertility(Avicenna Research Institute, 2015) Nsonwu-Anyanwu, A. C.; Charles-Davies, M. A.; Taiwo, V. O.; Bin, L.; Oni, A. A.; Bello, F. A.Background: Genital Chlamydia infection (GCI) and the associated pathologies have been implicated in tubal infertility. Though the actual pathologic mechanisms are still uncertain, oxidative stress and other factors have been implicated. The purpose of the study was to determine the possible contribution of female reproductive hormones and biomarkers of oxidative stress in genital Chlamydial infection to tubal occlusion. Methods: This prospective case control study was carried out by recruiting 150 age matched women grouped into infertile Chlamydia positive women (n=50), fertile Chlamydia positive women (n=50) and fertile Chlamydia negative women as controls (n=50). High vaginal swabs and endocervical swabs were collected for screening Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Treponema pallidum, Staphylococcus aureus, and Candida albicans. Sera were collected for estimation of Chlamydia trachomatis antibody, female reproductive hormones [Follicle Stimulating Hormone (FSH), Luteinizing Hormone (LH), Oestradiol (E2), Progesterone (P4), Prolactin (PRL)] and biomarkers of oxidative stress [Total Antioxidant Capacity (TAC) and 8-hydroxyl-2-deoxyguanosine (8-OHdG)] by enzyme immunoassay (EIA). Data were analyzed using chi square, analysis of variance and LSD Post hoc to determine mean differences at p=0.05. Results: Among women with GCI, higher levels of LH and 8-OHdG were observed in infertile Chlamydia positive women compared to fertile Chlamydia positive women (p<0.05). Higher levels of LH and 8-OHdG and lower TAC levels were observed in infertile Chlamydia positive women compared to fertile Chlamydia negative controls (p<0.05). Conclusion: Mechanisms including oxidative DNA damage and reduced antioxidant capacity may be involved in the pathology of Chlamydia induced tubal damage.Item Chlamydial infection, plasma peroxidation and obesity in tubal infertility(2011-12) Nsonwu-Anyanwu, A. C.; Charles-Davies, M. A.; Oni, A. A.; Taiwo, V. O.; Bello, F. A.Background: Genital tract infections and obesity are both sources of oxidative stress. Alterations in immune and antioxidant parameters may arise from this or from an indeterminate autoimmune mechanism. Objective: This study aimed to investigate the association of Chlamydial infection, obesity and oxidative response with tubal infertility in Nigerian women. Methods: It was a case-control study of 40 women with tubal infertility and 32 fertile women, respectively, recruited from the Infertility and Family Planning Clinics respectively, of the University College Hospital, Ibadan, Nigeria. Anthropometric indices were measured in each subject and endocervical swabs were taken to screen for current genital tract infection. Antioxidant, hormonal and immunologic analysis were performed on serum. Results: None of the subjects had current genital tract infections. Chlamydia trachomatis IgG positivity was significantly higher in infertile than in fertile subjects [OR 4.33; 95%CI (0.078-0.681)]. No significant variations were observed in the anthropometric indices, antioxidant parameters and hormones between infertile and the fertile women. Body mass index correlated positively with oxidative stress in infertile subjects. Waist and hip circumferences correlated negatively with oestradiol in women with tubal infertility. Conclusion: Chlamydial infection is associated with tubal factor infertility, however, obesity seems to increase oxidative stress and reduce fertility potential in women with tubal factor infertility.Item Genital ulcer diseases among HIV_infected female commercial sex workers in Ibadan, Nigeria(2011) Fayemiwo, S. A.; Odaibo, G. N.; Oni, A. A.; Ajayi, A. A. AWe evaluated the prevalence and association of Genital Ulcer Diseases (GUDs) among HIV-1 infected female commercial sex workers (FCSWs) in Ibadan, Nigeria. A total of 250 FCSWs from brothels in Ibadan were tested for presence of antibodies to HIV and Syphilis. Pelvic examinations for signs of sexually transmitted infections (STIs) were carried out on the subjects. Endocervical and high vaginal swabs were collected from each of the subjects to establish laboratory diagnosis of STIs. Their age ranged from 15 to 55 years (Mean = 25.8 yrs; SD = 3.74). Majority (246/250) were Nigerians, while 1.6% were from neighboring West African countries. Sixty four (25.6%) of the subjects were positive for HIV-1 while seven (2.8%) had dual HIV-1/2 infection. Analysis of the STIs showed that 49 (19.6%) of the CSWs had GUDs. Herpes genitalis was the commonest GUDs as it occurred in 25 (10%) of the subjects. Other STIs identified were chancroid (5.6%), syphilis (4.0%) and lymphogranuloma venerum (LGV) (4%). Sixteen (64.0%) of the CSWs with herpes genitalis had HIV-1 infection. The risk ratio of herpes genitalis for HIV acquisition was 3.0 (95% CI: 2.0 - 4.4). Syphilis and chancroid were also found tobe significantly associated with increased risk of HIV infection (p < 0.0001). The adjusted odd ratios for Herpes genitalis, chancroid, and syphilis were 3.7 (1-13.0, p < 0.05), 19.8 (2.7-13.0, p < 0.05) and 19.1 (1-231.0, p < 0.05) respectively. There is need to educate FCSWs continually to adopt safer sexual behaviours, seek early diagnosis and treatment of GUDs to reduce their risk of transmitting HIV infection.Item Hepatitis B surface antigen (HbsAg) in blood and gential secretions of paients with sexually transmitted diseases in Ibadan, Nigeria.(2005) Ogunkunle, M. O.; Oni, A. A.; Odaibo, G. N.; Olaleye, O. D.A total of 100 patients attending the Special Treatment Clinic of the University Teaching Hospital (UCH), Ibadan between October, 1998 and April, 1999 were studied to detect Hepatitis B surface antigen (HbsAg) in the blood and genital discharges. This was with a view of establishing whether infected persons (positive by blood test) also excrete the antigen, HbsAg, in their genital secretions. Urethral swabs were collected from 63 male patients, while High Vaginal and Endocervical swabs were collected from 37 female patients. Blood samples were collected from all the patients. HbsAg was tested for by Enzyme immunoassay technique with Wellcozyme HbsAg kit. Of the 63 male patients, 10 (15.9%) had HbsAg in the urethral secretion while 22 (34.9%) had it in their blood, 70% of these male patients were within the age range 11-30 years. Of the 37 female patients, 34 (92%) had HbsAg in the Endocervical secretions, 6 (16.2%) of them had HbsAg in their blood. Eighty percent of the female patients with positive HbsAg in the genital secretions were within age range 21-40 years. This study documents that Hepatitis B virus can be transmitted sexually in this environmentItem Sero-prevalence of hepatitis C virus among patients attending STD clinic in Ibadan, Nigeria(2005) Oni, A. A.; Odaibo, G. N.; Ola, S. O.; Olaleye, O. D.; Bakare, R. A."In the tropics, hepatitis C virus (HCV) seroprevalence ranges from < 0.2% in whole Africa. A strong association between HCV and hepatitis B surface antigen (HBsAg)-negative chronic liver disease and hepatocellular carcinoma has been described. Hepatocellular carcinoma (HCC) is one of the most common cancers among Africans, and in Nigeria by 1970 the estimated rate was 6.6 per 100,000 populations per annum. Sexual transmission was regarded as a minor cause of HCV, the degree of which has not been properly evaluated in most environments. Since it has been established that sexual transmission is an important mode of acquisition of the infection, we therefore set out to find the seroprevalence of HCV among 95 patients attending sexually transmitted diseases (STD) clinic in University College Hospital, Ibadan, Nigeria with a view to recommending preventive and control measures of HCV in our community. The sera collected from these respondents were used for screening for syphilis using the VDRL test, and for HCV antibodies using the MONOLISA anti-HCV (Sanofi, Pasteur France). Mid-stream urine was collected from all participants, and urethral swabs from all male participants while endocervical and high vaginal swabs were collected from female participants. Ulcer swabs were collected from those with genital ulcers. The prevalence of HCV infection was found to be 37.9% in patients presenting with STDs. This comprised 38.9% of males and 61.1% females. This prevalence rate is very high compared with the rate in the general population and other “high-risk” groups in previous studies in the same environment. Factors associated with HCV infection in this environment include high heterosexuality, high level of education, and previous instrumentations such as in scarifications and termination of pregnancy. Prevention and control of STDs will definitely reduce HCV infection and hence the attendant consequences, particularly hepatocellular carcinoma, in our environment. "Item Pattern if sexually transmitted Disease among HIV-! Infected commercial sex workers in Ibadan, Nigeira(MEDIMOND, 2004) Fayemiwo, S. A.; Bakare, R. A.; Odaibo, G. N.; Oni, A. A.; Fasina, A. A.; Olaleye, D. O.; Sankale, J. L.; Kanki, P."This study evaluatcd the association of HXV-1 infection with some other sexually transmitted diseases (STDs) among fernaIe commercial sex workers. BIood samples were collected from 250 female commercial sex workers in Ibadan Oyo State, Nigeria and tested for the presence of HEV antibodies using ELISA and Western blot. Endocervical and high vaginal swab samples were also collected for microscopy and culture. The prevalence of HIV- 1 infection among CSWs in Ibadan, Nigeria was 25.6% with 2.8% having dual reactivity to HIV- 1 and HIV- 2. Bacterial vaginosis was the commonest STDs (32.8%) followed by herpes genitalis, vaginal candidiasis, gonorrhoea, trichornoniasis, chancroid,syphilis, tinea curiz, genital warts, Iympholgranuloma venerum (LGV) and scabics. Recurrcnt vaginal discharge, gcnital ulccr discascs as well as non-genital ulcer diseases (tinea curiz and scabies) were associated with increased risk of HIV infections. Access to prevention and prompt management of these STDs will reduce the spread of HIV."