FACULTY OF BASIC MEDICAL SCIENCES

Permanent URI for this communityhttps://repository.ui.edu.ng/handle/123456789/262

Browse

Author: search.filters.author.Onifade, A. A.

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Oxidative stress biomarkers and their relationship with testosterone in male auto mechanics in Ibadan, Nigeria
    (Sciencedomain International, London, 2016) Adekola, S. A.; Charles-Davies, M. A.; Onifade, A. A.; Okoli, S. U.
    Occupational exposure to mixed chemicals generates free radicals with inadequate antioxidants resulting in oxidative stress. Recently, hypogonadism in male auto-mechanics was associated with oxidative stress. Studies show that testosterone, a male hormone increases the activities of antioxidant enzymes. This study is aimed at evaluating the oxidative stress biomarkers and their relationship with testosterone in auto mechanics in Ibadan, Nigeria. Eighty-three males participated in this prospective cross sectional study after informed consent. Forty-three were male auto-mechanics, occupationally exposed to mixed chemicals in the mechanic community, Bodija, Ibadan (cases). Their mean (SEM) age and body mass index (BMI) were 42.5 (1.7) years and 23.8 (0.5) Kg/m2 respectively. They were age and BMI matched with 40 unexposed, apparently healthy males from the University College Hospital and environs (controls). Demography, social habits, anthropometry and gonadal status were obtained by standard methods. Serum obtained from blood (10 ml) collected from the participants was used for biochemical analyses. Testosterone levels were determined by enzyme immunoassay method (Immunometrics UK Ltd). Levels of total antioxidant capacity, total plasma peroxide (TPP), malondialdehyde (MDA), hydrogen peroxide (H2O2), glutathione peroxidase (GPX), superoxide dismutase (SOD), glutathione-S-transferase (GST), and reduced glutathione (GSH) were determined using spectrophotometric methods while oxidative stress index (OSI) was calculated. P<0.05 was regarded as significant. TPP, MDA, OSI, H2O2 and GST `levels were significantly higher (P<0.001) in eugonadal cases compared with controls. All these biomarkers levels were similar in hypogonadal compared with eugonadal cases. (P>0.05) Testosterone related negatively with SOD in the controls only but positively with MDA and negatively with GST in cases only (P<0.05). Occupationally exposed auto mechanics appear to have oxidative stress and may benefit improvement in antioxidant status. Testosterone may contribute to and enhance total antioxidant status, which may be important in gonadal function.
  • Thumbnail Image
    Item
    Hypogonadism in males exposed to mixed chemicals in a mechanic village in Bodija, Ibadan
    (2015) Okoli, S. U.; Charles-Davies, M. A.; Onifade, A. A.; Adekola, S.
    Africa has great intensity of chemical exposure and high level of infertility. Functional disorders of the male germ cell and endocrinopathies have been attributed to exposure to mixed chemicals. Data on the mixed chemical exposure on reproductive hormones are sparse in Nigeria. This study was designed to evaluate male reproductive hormones and determinants of occupational exposure to mixed chemicals in a mechanic village in Ibadan. Forty-three males, auto mechanics aged 18-60 years occupationally exposed to mixed chemicals (MCG) at their work place in Bodija mechanic village with mean±SEM duration of 21.2±1.9 years were age matched with 40 unexposed males (controls) from the University College Hospital, Ibadan and environs. Demography, anthropometry, blood pressure and sexual history were obtained by standard methods. Blood (10 ml) was obtained from each participant for estimation of reproductive hormones by enzyme linked immunosorbent assay while total antioxidant capacity (TAC) was determined spectrophotometrically. Testosterone/oestradiol ratio (TE ratio) was calculated. The MCG were classified based on their reproductive hormone levels into 4 subgroups as eugonadic, hypogonadotrophic hypogonadism, sub-optimal hypogonadism and compensated hypogonadism. P<0.05 was regarded as significant. There were significantly raised oestradiol levels, diastolic blood pressure, waist circumference and waist to hip ratio and significantly lower TAC and TE ratio in the MCG than the controls (P<0.01). Exposed hypogonadic males exhibited significantly lower LH, FSH, testosterone, prolactin, TAC levels and TE ratio than exposed eugonadic males (P<0.05). Hypogonadism in the males exposed to mixed chemicals may be associated with reduced TE ratio resulting from increased adipose mass as well as oxidative stress associated with the mixed chemical exposure.