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Item Prevalence of eustrongylides ignotus in poecilia reticulata obtained from some wastewater drains of Lagos state(Fisheries Society of Nigeria, 2012-11) Akinwale, M.M.A.; Hassan, A. A.Poecilia reticulata is a common ornamental tropical fish‘is fouhd in many wastewater drains in Nigeria. Guppies feed on Oligochaetes which are intermediate hosts of Eustrongylides ignotus which is a parasite of Clarias gariepinus. The potential ban on the importation of ornamental fishes into Nigeria due to dearth of information on the parasite fauna of ornamental fishes .has enhanced the demand for local species, usually sourced from the wild. Sampling was done monthly using a 2 mm mesh-sized scoop net along selected drains at Igi-Olugbin Street, Basil Ogamba Street,, Ahmadu Bello Road and Adenaike Alagbe Street between March, 2004 and February, 2005. The selected drains were abutting residences and varying industrial activities in each of four different local government areas of Lagos State. 60 female and 60 male samples were randomly selected from each drain for dissection and microscopy. Nematodes observed were identified using standard identification guides. Prevalence was determined as percentage infection in guppies examined. Measurements of wastewater temperature, Dissolved Oxygen (DO), pH and transparency were done according to APHA methods: Drain depth was determined using a calibrated pole. Data was analyzed using chi-square test, ANOVA and Pearson correlation coefficient. Out of 4,320 n samples examined only 156 were infected. There was no sex-related difference in the prevalence of E. ignotus and no significant difference in mean monthly pH and DO across drains. The highest prevalence of 12.0% was observed in P. reticulata males obtained from Adenaike Alagbe . Street in June, 2004. Mean monthly physicochemical parameters for drains were: temperature, 25.0 ± 1.1- 26.0 ± 1.1 0 C; DO, 7.8 ± 2.1 - 8.4 ± 1.8 mg 1; pH, 6.9 ± 0.5 - 7.3 ± 0.4; transparency, 3.5 ± 0.8 - 23.0 ± 3.6 cm and drain depth, 9.6 ± 2.3 - 14.8 ± 3.2 cm. The prevalence of E. ignotus in the P. reticulata obtained from the selected Streets should not prevent their exploitation for both import substitution and the export market because it is lowItem Prevalence of camallanus cotti in poccilia reticulata obtained from some wastewater drains in Lagos state(Fisheries Society of Nigeria, 2012-11) Akinwale, M.M.A.; Hassan, A. A.One way to diversify Nigeria’s economy is to improve on agricultural activities with low import input but high export potential such as the promotion of the ornamental fish industry. P. reticulata is one such fish species that enjoys international acceptability. However, a potential bottleneck in its international trade is the decertification of exports from Nigeria in the uncertainty of standing parasite fauna of locally available stock of P. reticulata. Four Streets in different local government areas of Lagos State were identified with existing populations of P. reticulata where collection of samples with a scoop net was done before sorting into 60 male and 60 female individuals per wastewater drain from each Street was carried out monthly between March, 2004 and February, 2005. Temperature, pH, Dissolved Oxygen (DO), water transparency and drain depth were measured with mercury-bulb thermometer, ARH-1 CE electronic meter, Oxyguard electronic probe, secchi disc and calibrated pole respectively. Chi-square was used to test for sex-related differences in prevalence and ANOVA for differences in physicochemical analyses. Only 3.4% of the 4,320 samples examined were infected with C. cotti. There was no sex-related differences in the prevalence of C. cotti while only DO, transparency and drain depth were found to be significantly different among the wastewater drains on the four Streets. The highest prevalence of 15.0% was obtained in P. reticulata females obtained from Igi-Olugbin and Adenaike Alagbe Streets in September, 2004. High prevalence clustering correlated with receding rains. However, mean monthly temperature, transparency and drain depth were significantly different across drain (p < 0.05). P. reticulata obtained from these four Streets can be further exploited for import substitution and export market since this prevalence level can be managed and does not rival records of C. cotti in other popular export sources in AsiaItem SEM study on morphology and surface topography of wenyonia minuta woodland 1923 (cestoda: caryophyllidea) and the histopathological consequences on synodontis filamentosus (boulenger, 1901) from Lekki Lagoon, Lagos - Nigeria(2015) Akinsanya, B.; Hassan, A. A.; Adeogun, A. O.The SEM study on m orphology and surface topography of Wenyonia minuta was undertaken. The parasite was recovered from the intestine of Synodontis filamentosus. A total of two hundred and forty fi ve specimens of the fish host were exam ined.The fem ale specim ens (n =88) has a prevalence of 69.3% while the male specimens (n = 157) had a lower prevalence of 46.4%. The SEM description of the caryophyllidea cestode revealed the scolex to be fluke - like with four shallow longitudina l groove and flat pointed anterior end. The overall length of the scolex is 104250.97pm with a breadth of 653451.20pm .The furrow longitudinal groove were only observed on the dorsal side of the scolex. External protuberances were also reported in this study. The histopathological analysis shows m atting, lym phocytic infiltration of the lamina propria, goblet cell hyperplasia while some sections with no significant pathological changes were also observedItem Raised iron levels in wet- ground vigna unguiculata and capsicum frutescens using domestic grinding techniques(2014) Hassan, A. A.; Salawu, M. A.The biosafety of commonly used domestic grinding techniques was investigated; the effects of attrition mills using new (attrition mill 1) and old (attrition mill 2) plates, wooden mortar and pestle, grinding stone and electric blender on iron content of wet-ground staple foods, Vigna unguiculata (cowpea) and Capsicum frutescens (pepper) were examined in this study. Attrition mill 1 was in use 4 weeks prior to this study while the attrition mill 2 had newly installed grinding plate. The wet-ground pepper and cowpea were analyzed using Atomic Absorption Spectrophotometer (AAS). The iron contents of wet- ground foods (pepper and cowpea) from both attrition mills were significantly higher (p>0.05) than iron content of ground food using other methods of grinding. A 30-40 folds increases in the iron content of ground food samples were detected using attrition mills. Ground pepper from attrition mill 1 showed higher iron contents (4300±474.35mg) than pepper ground in attrition mill 2 (3199±281.68mg). These values are higher than recommended dietary allowances for iron intake. The increased iron content in ground pepper and cowpea observed in the present study confirmed the high risk of iron overloading using attrition milling. The level of contamination of ground food increased with use in attrition mills as a result of wear and tear of grinding plates.