Scholarly works in Virology
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Item Occult hepatitis B Virus Infection among HIV positive patients In Nigeria(Hindawi Publishing Corporation, 2014) Opaleye, O. O.; Oluremi,A, S.||Atiba, A. B; Oluremi,A, S.||Atiba, A. B; Adewumi, M. O.; Mabayoje, O. V.; Donbraye, E.; Ojurongbe, O.; Olowe, O. A."HIV has been known to interfere with the natural history of hepatitis B virus (HBV) infection. In this study we investigate the prevalence of occult hepatitis B virus infection (OBI) among HIV-infected individuals in Nigeria. Overall, 1200 archived HIV positive samples were screened for detectable HBsAg using rapid technique, in Ikole Ekiti Specialist Hospital. The HBsAg negative samples were tested for HBsAg, anti-HBc, and anti-HCV by ELISA. Polymerase chain reaction was used for HBV DNA amplification and CD4 counts were analyzed by cytometry. Nine hundred and eighty of the HIV samples were HBsAg negative. HBV DNA was detected in 21/188 (11.2%) of patients without detectable HBsAg. CD4 count for the patients ranged from 2 to 2,140 cells/𝜇L of blood (mean = 490 cells/𝜇L of blood). HCV coinfection was detected only in 3/188 (1.6%) of the HIV-infected patients(𝑃 > 0.05). Twenty-eight (29.2%) of the 96 HIV samples screened were positive for anti-HBc. Averagely the HBV viral load was <50 copies/mL in the OBI samples examined by quantitative PCR. The prevalence of OBI was significantly high among HIV-infected patients.These findings highlight the significance of nucleic acid testing in HBV diagnosis in HIV patients.Item HBV Infection among HIV-infected cohort And HIV-negative Hospital attendees in South Western Nigeria(African Network for Infectious Diseases (ANID), 2014) Adewumi, M. O.; Donbraye, E.; Odaibo, G. N.; Bakarey, A. S.; Opaleye, O. O.; Olaleye, D. O."Background: Prevalence, association and probable mode of acquisition of HBV and HIV dual infections have not been fully explored. Thus, HBV intervention plan and services are sometimes exclusively targeted towards HIV-infected population. We investigated HBV infection among HIV-infected cohort in comparison with HIV-negative hospital attendees to ascertain dual infectivity pattern; thereby encouraging appropriat allotment of intervention services. A total of 349 (M=141; F=208; Mean=33.98 years; Range= 0.33-80 years) plasma specimens from two virus diagnostic laboratories in south-western Nigeria were analysed. These include 182 HIV-positive and 167 HIV-negative specimens from ART and GDV laboratories respectively. The specimens were initially screened for detectable HIV antigen/antibody, and subsequently HBsAg by ELISA technique. Overall, HBsAg was detected in 20.92% (95% CI: 16.65-25.19%) of the patients. Also, 24.82% (95% CI: 17.69 31.95%) and 18.27% (95% CI: 13.02-23.52%) HBsAg positivity was recorded for males and females respectively. CHI square analysis showed no association (P=0.14) between gender and prevalence of HBsAg. Similarly, comparison of prevalence of HBsAg by age groups shows no significant difference (P=0.24). Overall, no significant difference (P=0.59) was observed in the prevalence of HBsAg among the HIV-infected cohort and HIV-negative hospital attendees. Results of the study confirm endemicity and comparable rates of HBV infection independent of HIV-status.Item Polio Virus neutralizing antibody dynamics among Children In A North-Central And South-Western Nigeria State(Talor&Francis, 2014-09-01) Adeniji, J. A.; Onoja, A. B.; Adewumi, M. O.Northern Nigeria accounts for the highest number of confirmed wild polio viruses globally. Transmission to neighboring countries is worrisome after the country failed to meet several deadlines for polio eradication. Most studies on neutralizing antibody have focused on the Northeastern and Northwestern regions. This study measured polio virus neutralizing antibody levels among children in North-central and South-western Nigeria. Children between the ages of 10 months and 13 yr were randomly selected from Abanishe-lolu Hospital Ilorin (North-central) and Oni Memorial and Adeoyo Hospitals in Ibadan (South-west) Nigeria. The alpha neutralization method was employed. Herd immunity was 1.4% in Ilorin, 36.6% in Oni Memorial Hospital, and 49.5% in Adeoyo Hospital. Out of 299 children studied, 49 (16.4%) children had no protection against all poliovirus serotypes while 100 (33.4%) were fully protected against all three serotypes. Five (7.9%) children in Ilorin and 5 (3.4%) children in Oni Memorial Hospital Ibadan had no detectable neutralizing antibody. A significant difference was observed (p=0.000) when the GMT against poliovirus 1, 2, and 3 was compared. A significant proportion of children were not fully protected. Sero-surveillance is recommended for effective monitoring of vaccination efforts to guide health policy formulators.Item Rubella igg antibody among Nigerian pregnant women without vaccination history(Pan African Society for Clinical and Experimental Microbiology, 2013-01) Adewumi, M. O.; Olusanya, R. B.; Oladunjoye, B. A.; Adeniji, J. A."Rubella is a vaccine-preventable viral infection, its aetiologic agent; rubella virus was identified as human teratogen capable of causing a spectrum of birth defects described as congenital rubella syndrome (CRS). Despite the availability of safe and effective vaccines, significant proportion of the population remains susceptible to rubella infection in developing countries. More significantly, such developing countries including Nigeria have not demonstrated adequate commitment to preventive vaccination; a panacea for intervention. Consequently, this study was designed to determine the prevalence of anti-rubella IgG among pregnant women to ascertain the proportion of susceptible population. A total of 273 consenting rubella vaccine naïve antenatal clinic attendees aged 15-42 years (Median age = 28 years) were randomly selected and their sera analyzed for qualitative and quantitative anti-rubella IgG detection. Overall, 244/273 (89.4%) pregnant women enrolled in this study had protective level (Titre = >10 IU/mL) of anti-rubellaIgG (Median Titre = 165 IU/mL; Range = <10 - >250 IU/mL), while, 29/273 (10.6%) of the study population lack protective antibody titre ( OD = <10 IU/mL). Results confirm previous reports of exposure, infection, and continuous circulation of rubella virus in Nigeria. It emphasizes the need for improved and continuous surveillance for rubella and CRS cases, prompt vaccination of vulnerable populations, and evaluation of health policies to achieve immunization and ultimately ensure control/elimination of rubella virus in Nigeria and beyond."Item High Prevalence Of Anti-Hepatitis A Virus Immunoglobulin G Antibody Among Healthcare Facility Attendees In Osogbo, Nigeria(Talor&Francis, 2013-01-19) Sule, W. F.; Kajogbola, A. T; Adewumi, M. O.The objectives of this study were to determine the prevalence of anti-hepatitis A virus (HAV) immunoglobulin G (IgG) antibody and associated factors among residents of Osogbo, a town in Nigeria with inadequate environmental sanitation and a shortage of potable water. This is a health facility–based study. Ninety one consenting, asymptomatic attendees of public healthcare facilities in Osogbo, Osun State, Nigeria, were consecutively selected for this study. Plasma samples of the study participants were tested for the presence of anti-HAV IgG using a HAV Ab Competitive Enzyme Immunoassay test kit. Ninety (98.9%) of the participants were seropositive for anti-HAVIgG antibody; group-specific prevalence was also high, but association of participants’ variables with the prevalence could not be obtained due to limited sample size and high group-specific prevalence. Since the hepatitis A vaccine is not currently used in Nigeria, the chance is high that the HAV IgG antibody–positive individuals were naturally infected; consequently Osogbo can be described as highly endemic for HAV infection.Item Hepatitis B Core IgM antibody (anti-HBcIgM) among Hepatitis B Surface antigen (HBsAg) negative blood donors in Nigeria(Springer Nature, 2011) Japhet, M. O.; Adesina, O. A.; Donbraye, E.; Adewumi, M. O.Background: Transfusion associated Hepatitis B virus (TAHBV) continues to be a major problem despite mandatory screening for Hepatitis B surface Antigen (HBsAg). Presence of HBsAg is the common method for detecting hepatitis B infection. Unfortunately, this marker is not detected during the window period of the infection. Nigeria being a developing country cannot afford DNA testing of all collected units of blood which serve as the only possibility of achieving zero risk of transfusion associated HBV. Five different serological makers of hepatitis B virus (HBV) infection were therefore assessed to evaluate the reliability of using HBsAg marker alone in diagnosis of HBV infection among blood donors and to detect the serological evidence of the infection at the window period. This will preclude the possibility of transmitting hepatitis B through transfusion of Hepatitis B surface antigen (HBsAg) negative blood in Nigeria. Methods: Between July and August 2009, 92 blood donors were enrolled for the study. The prevalence of 5 different markers of Hepatitis B virus infection was detected using Enzyme Linked Immunosorbent Assay (ELISA). Demographic factors were assessed during the study.Results: HBsAg and its antibody (anti-HBs) was detected in 18 (19.6%) and 14(15.2%) of the 92 blood donors respectively. Anti-HBc IgM was found in 12(13.0%) of the 92 blood donors while Hepatitis B envelope antigen (HBeAg) and its antibody (anti-HBe) were detected in 4(8.9%) and 12(26.7%) respectively from 45 donors sampled. HBeAg is a marker of high infectivity and appears after HBsAg. At least one serological marker was detected in 30(32.6%) of the blood donors. Five (5.4%) of the 92 donors had anti-HBc IgM as the only serological evidence of hepatitis B virus infection. Conclusions: The result of this study shows that five donors have anti-HBcIgM as the only serological evidence of HBV infection. Inclusion of anti-HBcIgM in routine screening of blood donors in Nigeria should be encouraged. This is the first study to assess anti-HBcIgM in the country.Item Evaluation of immunity against poliovirus serotypes among children in riverine areas of Delta State, Nigeria(Medical Microbiology and Parasitology Society of Nigeria (MMSN), 2011-06-01) Donbraye, E.; Adewumi, M. O.; Odaibo, G. N.; Bakarey, A. S.; Opaleye, O. O.; Olaleye, D. O.Nigeria remains one of the major reservoirs for wild poliovirus transmission despite the reported success in National Immunization Days and acute flaccid paralysis surveillance. Two hundred children aged ≤ 10 years, were enrolled following parental consent from hard-to-reach riverine areas of Delta state of Nigeria to assess the level of protective immunity to poliovirus. Neutralizing antibodies to the three poliovirus serotypes in the serum samples of the children were determined by the beta method of neutralization. Eight (4%) of the children had no detectable antibody, 178 (89%), 180 (90%) and 181 (90.5%) were positive for antibodies to poliovirus types 1, 2 and 3, respectively. Overall, 162 (81%) of the children had antibodies to the three poliovirus serotypes at a titre of at least 1:8. The study shows the need for proper monitoring of vaccination coverage in such hard-to-reach riverine areas to achieve the objective of the global eradication of poliovirus.Item Traditional crop farmers in Kogi East, Nigeria elucidate elevated HIV and AIDS prevalence level during a five -year study period(Academic Journals (Academic Journals Inc., Lagos, Nigeria), 2009) Sule, W. F.; Enemuor, S. C.; Adewumi, M. O.; Attah, O. C.The United Nations reported that HIV and AIDS have negative impact upon agriculture and increasing hunger in sub-Saharan Africa. Such a situation is postulated to synonymously occur in Nigeria. This study therefore, aimed to investigate the prevalence level of HIV antibodies and AIDS among traditional crop farmers and non-farmers comprising traders, drivers, teachers and students in Kogi East. This is one of the primary agricultural areas of Kogi State, Nigeria and is therefore of great importance to perform an assessment depicting the prevalence of local HIV infection. Documented records of HIV antibody screening from the year 2002 to 2006 at the Voluntary Counselling and HIV Testing Unit of General Hospital, Ankpa, Kogi East were reviewed. Selected data was statistically analyzed with Chi2 - test using SPSS 13.0. A total of 11,077 patients from the ages of two months to 72 years were screened during the five years. Of this, 2,510 HIV positive cases (overall prevalence of 22.70%; mean = 21.86%; n= 5; standard deviation [SD] = 5.64%) and 322 mean = 12.47%; n = 5; SD = 2.23%) AIDS-related deaths were documented. The letter group included 250 (77.6%, mean = 71.91%; SD = 15.72%) farmers. A significant higher number of farmers were seropositive (_2 = 72.710; p = 0.001, df = 1, _ = 0.05) and died (_2 = 93.255; p = 0.001, df = 1, _ = 0.05) of AIDS-related illness compared to the non-farmers. The ages of people that died range from 12 to 49 years (n = 322) with a mean age of 44.1 years. Throughout the specified five years sampling duration, more non-farmers than farmers partook in HIV antibody screening. Farmers that were seropositive for HIV antibodies and died of AIDS-related illness were significantly higher in proportion than the non-farmers.Item Human immunodeficiency virus (HIV) specific antibodies among married pregnant women and female commercial sex workers attending voluntary counseling and HIV testing (VCT) centre in Abuja, Nigeria(Medical Microbiology and Parasitology Society of Nigeria (MMSN), 2009) Sule,W. F; Adewumi, M. O.; Samuel, T. C.Generally, married women and commercial sex workers (CSWs) engage in sexual activity - an important risk factor for contracting HIV. We therefore tested a hypothesis that prevalence of HIV-1/2 antibodies among married pregnant women (PW) is not different from that of female CSWs. One hundred married PW and 99 female CSWs enrolled in the study. They were consecutively selected as they visited the VCT centre for HIV antibody test. Pertinent data were obtained from each subject using questionnaire forms; venous blood sample was aseptically collected from subjects that gave verbal consent. Plasma obtained from each sample was tested using parallel testing algorithm with DETERMINE® HIV-1/2 and HIV-1/2 STAT-PAK® test was used for statistical analysis of the data. The overall prevalence of HIV-1/2 antibodies was 29.1% (n = 199). Seroprevalence of 39.4 and 19.0% were observed for the CSWs and the PW, respectively. Using various variables, comparison of HIV-1/2 serostatus of the CSWs with that of the married PW showed that the CSWs generally had significantly higher seroprevalence. CSWs who were inconsistent in the use of condom with their clients prior to sexual intercourse in the past three months before this study (P = 0.0001, OR = 11.2) and those aged _ 39 years had significantly (P =0.004, OR = 2.6) higher seroprevalence. Though both groups recorded seropositivity, inconsistency in the use of condom appeared to be the factor mostly responsible for the significantly higher seropositivity of the female CSWs.Item Reliability of testing and potential impact on HIV prevention in Nigeria(Medical and Dental Consultants Association of Nigeria (MDCAN), 2006) Odaibo, G. N; Donbraye, E.; Adewumi, M. O.; Bakarey, A. S.; Ibeh, M. A.; Olalcye, D. O.Several factors including variability of human immunodeficiency virus (HIV), laboratory facilities, cost and competence of personnel handling the tests are some of the important factors that affect accuracy and reliability of HIV testing in most parts of Africa. Recently investigators in Africa have observed that antibody detection assays based on antigens derived from HIV-1 subtype B show moderate to significantly lower sensitivity for detection of infection by various non-B subtypes. In this study, we evaluated the reliability of two El A and 12 rapid HIV-1/2 test kits that are commercially available in Nigeria using the Western immune blotting technique as reference. A panel of 100 sera from Western blot confirmed symptomatic or asymptomatic HIV-1 infected persons and 90 seronegative patients from those referred for testing in our laboratory were used for this study. Each sample was tested with two HIV-1/2 EIA, and 12 HIV-1/2 rapid test kits commercially available at one time or the other for HIV-1/2 testing in Nigeria. Overall, the sensitivity of the two EIA kits were 100% and 91.0% with specificity of 96.7% and 91.1% respectively. The sensitivity of the rapid test kits ranged from 88% to 98.0% with specificity of 92.2% to 100%. Further analysis showed significant variation in the sensitivity and specificity of the same kit based on whether an individual had asymptomatic or symptomatic infection the results of this study highlight the problem of diagnosis of HIV infections in Africa. It shows that the sensitivity of most of the rapid assays shall not be adequate for detection of early infection. The implications of possible misdiagnosis on the various intervention strategies that rely predominantly on correct HIV status of an individual are enormous. Thus, there is an urgent need for review of the current HIV testing assays or algorithms in Nigeria and other parts of Africa.