Pharmaceutical Microbiology
Permanent URI for this communityhttps://repository.ui.edu.ng/handle/123456789/367
Browse
9 results
Search Results
Item Molecular screening of antibiotic-resistant determinants among multidrug-resistant clinical isolates of proteus mirabilis from SouthWest Nigeria(Makerere University Medical School (Uganda), 2017) Alabi, O. S.; Mendonça, N.; Adeleke, O. E.; Da Silva, G. J.Background: Globally, and particularly in developing countries, the menace of anti-microbial resistance is an accelerating problem. In Nigeria, increase in bacterial resistance has been phenotypically established but due to high cost, few molecular studies have been reported. Objectives: This study screened for presence of transferable resistance genes and mobile genetic elements (MGEs) such as integron among multi-drug resistant (MDR) P. mirabilis. Methods: A total of 108 P. mirabilis strains collected from five tertiary hospitals in SouthWest Nigeria were subjected to antibiotic susceptibility study using disc-diffusion method. Transferable resistance genes and MGEs were amplified using Polymerase chain reaction (PCR) analysis and amplicons sequenced. Results: Varied resistance was observed against all the antibiotics tested. About 56% of the isolates were MDR including those from 0-12 years old children. PCR analysis revealed the presence of aac(6’)-Ib (33.3%), plasmid mediated quinolone resistance (PMQR) genes [qnrA (36.7%), acc(6’)-Ib-cr (5%)], TEM (48.3%), CTX-M (6.7%) and integrons class 1 (58.3%) and class 2 (26.7%). Sequencing analysis revealed blaTEM-1, blaCTX-M-15 associated with ISEcp1 and eight different arrays of gene cassettes: aadA1, aadA1-qacH, aadB-aadA2, aadA5, dfrA7, dfrA15, dfrA17, dfrA17-aadA5. Conclusion: Transferable resistance genes in association with MGEs are present in Nigerian P. mirabilis thus their potential in disseminating resistance.Item Effect of kunnu-zaki on clinical bacteria isolates(Academicjournals, 2012-03) Olaitan, J. O.; Adeleke, O. E.; Alabi, O. S.Ten clinical isolates of Gram-negative bacteria were exposed in agar-cup diffusion sensitivity test to Kunun-zaki, a non-alcoholic fermented beverage, taken from eighteen samples. The undiluted samples of the beverage appreciably inhibited the growth of all the bacteria tested including, remarkably, Pseudomonas aeruginosa, Vibro cholerae, Salmonella enterica and Staphylococcus aureus. The bacteria however varied in their sensitivities to the fractional dilutions of the samples. This antimicrobial effect represents another property for Kunun-zaki and obviates the possibility of public health risk in its consumption.Item Multidrug resistant and extended-spectrum beta-lactamase (ESBL) producing proteus mirabilis from tertiary hospitals in four states in Southwest Nigeria(Nigeria Association of Pharmacists in Academia (NAPA), 2015) Alabi, O. S.; Adeleke, O. E.; Adegoke, O. A.; Gbadeyan, O. F.; Ejilude, O.Background: Multidrug resistant (MDR) and extended-spectrum beta-lactamase (ESBL) producing Gram-negative bacteria pose great threat to antibiotic treatment of life threatening infections worldwide. Objectives: This study investigated the occurrence and distribution of MDR and ESBL producing Proteus mirabilis among clinical isolates collected from tertiary hospitals in four states in Southwest Nigeria. Materials and Methods: One hundred and eight (108) none-duplicated P. mirabilis collected from microbiology units of tertiary hospitals in four states in Southwest Nigeria namely; Oyo, Osun, Ogun and Lagos state, after authentication with standard bacteriological method, were subjected to antibiotic susceptibility test against ten selected antibiotics using disc-diffusion method. Presumptive production of ESBL was determined by double disc synergy test among isolates with MDR phenotype that showed resistance to any of the third generation cephalosporin antibiotics. Results: Of the 108 clinical isolates of P. mirabilis collected from Oyo (39.8%), Osun (25.9%), Ogun (21.3%) and Lagos (13%) states, 60 (55.6%) showed MDR phenotype. Among the 60 MDR isolates collected in Oyo (50%), Lagos (10%), Ogun (21.7%) and Osun (18.3%), 66.7%, 66.7%, 30.8% and 9.1% of the isolates produced ESBL, respectively. Conclusion: This study recorded the occurrence of ESBL and MDR P. mirabilis in all the four states but higher percentage of ESBL-production among MDR P. mirabilis in two of the states, Oyo and Lagos. Hence, there is need for adequate monitoring of antibiotic use to prevent increased rate of ESBL-positive MDR P. mirabilis in these states and others in the nearest future.Item Prolonged use of cough formulations and the health risk from their antimicrobial activity on some normal bacterial flora(AJCEM, 2010-01) Adeleke, O. E.; Alabi, O. S.; Adetoyi, O. A.Cough formulations were observed to contain some chemical substances that have been associated with antimicrobial property, namely: menthol, honey, citric acid and volatile oils. A prolonged use of such formulations by patients was therefore considered a health risk on the normal bacterial flora. Nine cough formulations denoted by letter codes along with simple syrup B.P., absolute alcohol and sterile distilled water as controls, were investigated for relative antimicrobial activity on some normal flora bacteria by the agar-cup diffusion method. The respective individual single brands of cough formulation with the exception of one brand exhibited inhibitory activity against 5 - 1 2 bacterial isolates including Escherichia coli, Klebsiella spp, Streptococcus faecalis, Strep. pneumoniae, Strep. viridians, Proteus mirabilis, Staphylococcus aureus and Pseudomonas aeruginosa. Simple Syrup B.P. and sterile distilled water did not exhibit any antibacterial activity while the absolute alcohol exerted activity only on Staph aureus. The antimicrobial activity recorded for the cough formulations could cause a depletion of the normal bacterial flora following a prolonged use of the formulations tested, hence, the attendant health risk of depressed natural immune system of the body, normally associated with such bacteria. pathological status when it presents as aItem Antibiotic resistance profile and phenotypic detection of betaLactamase-producers among gram-negative bacteria isolated from(Nigeria Association of Pharmacists in Academia (NAPA), 2016) Adejumo, A. E.; Alabi, O. S.; Adeleke, O. E.Background: Cockroaches are carriers of numerous microorganisms. However, there is paucity of information on their role as potential reservoir for beta-lactamase producers. Objectives: This research determined the antibiotics susceptibility profile of Beta-lactamase producing Gramnegative bacteria isolated from the gut of household cockroaches in selected locations in and around University of Ibadan, Oyo state. Materials and Methods: Thirty Cockroaches from different locations in and around University of Ibadan were collected between June 2015 and March, 2016, and their intestinal homogenates cultured on different selective media for the isolation of bacteria. The isolates were identified using a combination of biochemical tests and 16S rRNA sequencing. Antibiotic susceptibility testing was done using the disc-diffusion technique and phenotypic detection of extended-spectrum beta-lactamase (ESBL), AmpC-beta-lactamase (AmpC) and Metallo-beta-lactamase (MBL) production was done using double-disc synergy, cefoxitin/cloxacillin and imipenem/EDTA double-disc methods respectively. Results: A total number of 58 bacteria belonging to nine genera; Pseudomonas, Proteus, Klebsiella, Salmonella, Enterobacter, Escherichia, Serratia, Shigella and Raoultella were isolated. Higher percentage of the isolates exhibited resistance to erythromycin (90%), azithromycin (87.5%), amoxicillin (84.5%), ampicillin (74.1%), amoxicillin-clavulanic acid (74.1%), cefoxitin (67.2%) and chloramphenicol (54%), while lower percentage showed resistance to aztreonam (25%), ertapenem (13%), cefotaxime (8.6%), ceftazidime (10.3%), cefepime (5.2%), ciprofloxacin (3.5%), gentamicin (5.2%) and imipenem (0%). MDR phenotype was recorded among 82% of the isolates, 17.2% were positive for ESBL, 12% for AmpC and 13.8% for MBL production. Conclusion: This study identified household cockroaches Periplaneta americana, as a potential reservoir for MDR and beta-lactamase-producing isolates. Keywords: Susceptibility testing, ESBL, AmpC, MBL, Beta-Lactamase, MDR, Household cockroaches Cockroaches are insects of the order Blattodea some of which are associated with human environment (Beccaloni, 2014). One of the two species of cockroaches that has been reported by different authors to be widely distributed in Nigeria is the American cockroaches namely Periplaneta americana (Ajero et al., 2011; Etim et al., 2012; Akinjogunla et al., 2012; Tilahun et al., 2012). The incidence of Cockroach infestation in restaurants, hospitals, warehouses, offices and within households, particularly the kitchen, have been reported to be very high in Nigeria (Omudu and Akosu, 2013; Braimah et al., 2015). The insects being nocturnal in nature are found in dark places within the households during the day hiding in opened and cracked sections of the wall, furniture, inside wardrobes and cupboards (Omudu and Akosu, 2013; Billah et al., 2015; Braimah et al., 2015). Sometimes they enter into cooking pots and other kitchen utensils if left opened. They feed on leftover food, cooked and uncooked food materials contaminating them with microorganisms on their body and in their faecal droppings especially those ready to eat food that do not require further processing before eating them (Xue et al., 2009). They are also found in other parts of the house including bedrooms (Etim et al., 2013). Cockroaches are also found in garbage and sewage tanks, particularly in homes with pit latrine (Craczyk et al., 2005; Pai et al., 2005) making them a potential vector for pathogenic and non-pathogenic microorganisms from human origin (Graczyk et al., 2005; Pai et al., 2005; Vazirianzadeh et al. 2014). Studies have shown that most of the disease-causing bacteria of human origin can survive within the body system of cockroaches (Imamura et al., 2003), hence their presence in sewage and garbage elucidate their role as potential carriers of these human diseasecausing agents (Vazirianzadeh et al., 2014). Several authors have incriminated cockroaches as reservoir and transmitters of disease-causing microorganisms like Enterobacter spp., Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Shigella spp., Campylobacter spp. and Salmonella spp., especially those in the hospital environment (Craczyk et al., 2005; Tatfeng et al., 2005; Ukay et al., 2009; Tilahun et al., 2012; Vazirianzadeh et al., 2014). In addition, cockroaches have also been reported to carry microbial agents of leprosy, urinary tract infection (Enterococcus spp.), MATERIALS AND METHODS Study Area The study was carried out in University of Ibadan metropolis, Oyo state. The areas of choice were kitchen and toilets in residential apartments in and around the University of Ibadan. Specimen Collection and Preparation for bacteria isolation A total of 30 cockroaches, identified to be Periplaneta americana, through online file (Beccaloni, 2014), were collected from residential cholecystitis (Helicobacter hepaticus), plague (Yersinia pestis) and bacteremia, septic arthritis and peritonitis (Oligella urethralis) (Pat, 2006; Falsafi and Mahboubi, 2013). Antibiotic resistance and its spread among bacterial isolates is a global problem and has been reported among bacterial isolates in both clinical and community settings (WHO, 2014). Cockroaches have been reported to harbour pathogenic and opportunistic pathogens that exhibited high level resistance to antibiotics, especially those used in lifethreatening disease cases such as the cephalosporins and carbapenems (Fathpour et al., 2003; Bouamama et al., 2010; Wannigama et al., 2014). Bacterial isolates exhibiting multidrug resistance phenotype have also been reported in cockroaches (Devi and Murray, 1991; Tetteh-Quarcoo et al., 2013). Resistance to beta-lactam antibiotics are known to be mediated mostly by beta-lactamase enzymes. The initial variants of these enzymes were active only on the penicillin class but the discovery of the third generation cephalosporin class help to overcome their destructive action (Paterson and Bonomo, 2005). The advent of extended-spectrum beta-lactamase, and metallo beta-lactamase enzymes among others which are known in addition to the penicillin class, to hydrolyze the third generation cephalosporins (Ceftazidime, cefotaxime, ceftriaxone) and the carbapenems (imipenem, ertapenem) respectively, created a serious problem for clinicians in the management of life-threatening infections caused by such bacteria habouring the enzymes (Paterson and Bonomo, 2005). Currently, there is paucity of information on the various resistant determinants harboured by bacteria isolated from household cockroaches. This study therefore, aimed at using phenotypic techniques to determine multidrug resistance phenotype and the presence of common beta-lactamase enzymes among bacterial isolates from the gut of house-hold cockroaches in selected locations in and around University of Ibadan. apartments using sticky rods and direct collection with sterile hand-gloves (Paul et al., 1992). They were introduced into separate sterile wide-mouth bottles aseptically and transported to the laboratory of the Department of Pharmaceutical Microbiology, University of Ibadan for freezing at 0oC for 10- 15min to anaesthetize them. Intestinal gut of each cockroach was prepared into samples using the method highlighted by Tetteh-Quarcoo et al. (2013)Item Serum protein binding and the antimicrobial activities of garcinia kola, kola acuminata and kola nitida seed extracts on the agents of respiratory tract infections(West African Society for Pharmacology, 2009) Adeleke, O. E.; Alabi, O. S.; Coker M. E.The kinetics of bactericidal activity of the methanolic extracts of powdered seeds of Garcinia kola (Heckel), Kola acuminata and Kola nitida obtained by soxhlet extraction, were studied using two bacterial sensitive strains of Staphylococcus aureus and Pseudomonas aeruginosa. The study showed that the three extracts at the concentrations used against the two test organisms, Staphylococcus aureus OOUTH206 (0.5mg/ml for Garcinia kola, 4mg/ml for Kola acuminata and Kola nitida) and Pseudomonas aeruginosa UCH189 (8mg/ml for Garcinia kola, 15mg/mi for Kola acuminata and Kola nitida); had activity on the two microorganisms. There was a gradual reduction in the number of viable microbial counts as evident in the reduction in the cell number at the different time intervals. Similar procedure was carried out in the presence of 5% serum protein and it was observed that the activities of the various extracts were altered significantly indicating the effect of serum protein binding on the Constituents of Garcinia kola, Kola acuminata and Kola nitida seed extracts. The results were expressed in percentage viable count of the inoculum size and showed that the percentage viable count decreases with time in varying degrees. The reduction was more intense with G. kola extract than the other extracts on S. aureus as well as on P. aeruginosa towards the third and fourth hours. The effect of serum protein was seen to affect the activities of all the extracts in varying degrees as shown in the graphs plotted.Item Elixir formulations of methanolic extract concentrates of garcinia kola, kola acuminata and kola nitida seeds and their antimicrobial activity(West African Postgraduate College of Pharmacists, 2008) Adeleke, O. E.; Alabi, O. S.; Coker M. E.The crude methanolic extracts of Garcinia Kola (Gutiferae), Kola acuminata and Kola nitida (Sterculiaceae) seed powders were screened against some bacterial isolates consisting of six different Genera namely: Streptococcus, Staphylococcus, Escherichia, Proteus, Klebsiella and Pseudomonas, most of which were isolated from the respiratory tract and the rest from other sources like wounds, vagina and urethra. The extracts were found to be active on all the Gram-positive bacteria and some of the Gram-negative bacteria including Pseudomonas aeruginosa. The extracts Were then formulated into stable Elixir formulations at a concentration of 15mg/ml and screened against the same bacterial isolates. They were found to be active against all the selected Gram-positive bacteria used in were also susceptible to the three elixir formulations.Item Comparative antimicrobial activities of extracts of garcinia kola, kola acuminata and kola nitida seeds on isolates of respiratory tract and other infections(West African Postgraduate College of Pharmacists, 2008) Adeleke, O. E.; Alabi, O. S.The methanolic extracts of the powdered seeds of Garcinia kola (Guttilerae), Kola acuminata, and Kola nitida (Sterculiaceae) were tested individually for antimicrobial activity using isolates from respiratory tract infections and other sites of infection such as High vagina swab, wounds, urethra and skin. The isolates included Staphylococus aureus (5strains), Z strains each of Escherichia coli, Proteus mirabilis and Streptococcus pneumoniae; I strain each of Streptococcus viridians Entercoccus faecalis, Streptococcus pyogenes, Bacillus subtilis, Haemophillus influenzae, Candida albicans, Asp. Niger, Ap. Eumigatus and Trichophyton specie, and 3 strains each of Pseudomonas aeruginosa and Klebsiella pneumoniae. The antimicrobial susceptibility test of the extracts showed zones of growth inhibition ranging from 9mm to as large as 35mm, depending on the organism being tested. The minimum inhibitory concentration (MIC) was determined by agar-diffusion method and ranged from 0.23mg/ml to 30mg/ml for the three extracts. The crude extracts of Garcinia Kola showed a stronger antimicrobial against most organisms particularly the Gram-positive bacterial isolates from the respiratory tract, than the other two extracts, while Kola nitida was the least active.Item performance of an acid-cassava starch medium in the propagation of fungi(1999) Adeleke, O. E.; Odelola, H. A.; Onasanya, Y.