FACULTY OF PHARMACY

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    Phytosterols from spondias mombin Linn with antimycobacterial activities
    (2013-01) Olugbuyiro, J. A. O.; Moody, J. O.; Hamann, M. T.
    The growing problems of tuberculosis have led to the search for new anti-Mtb agents from higher plants. The stem bark of Spondias mombin was evaluated for its in vitro activity against Mycobacterium tuberculosis (H37Rv strain). Bioassay-guided fractionation of the methanol extract was carried out by Vacuum Liquid Chromatography (VLC) on Silica gel (230-400 mesh) and purification was done using HPLC and TLC. In vitro antimycobacterial susceptibility was performed by a fluorometric microplate alamar blue assay (MABA) and percentage mycobacterial inhibition was calculated. The structures of the isolated compounds were established by spectroscopic analysis. The active VLC fraction exhibited 91% inhibition against M. tuberculosis H37Rv at a concentration of 40 μg/mL. The HPLC fraction SMi-15 containing compounds 1 and 2 showed 92.8% inhibition against M. tuberculosis. Two new antimycobacterial phytosterols were isolated from the stem bark of S. mombin and the structureswere identified as mombintane I (1) and mombintane II (2). The stem bark extractives of S. mombin contain antitubercular principles of the class phytosterol and support an important potential of triterpenoids.
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    In-Vitro Susceptibility of Mycobacterium Tuberculosis to Extracts of Uvaria Afzelli Scott Elliot and Tetracera Alnifolia Willd
    (2011-01) Lawal, T. O.; Adeniyi, B. A.; Wan, B.; Franzblau, S. G.; Mahady, G. B.
    Tuberculosis is a global burden with one –third of the world’s population infected with the pathogen Mycobacterium tuberculosis and an annual 2 million deaths from the disease. This high incidence of infection and the increased rate of resistant strains of the organism (MDR- and XDR- TB) have called for an urgent need to develop new anti-tuberculosis drugs from plants. The crude extract of Uvaria afzelli Scott Elliot (Annonaceae) root bark, and leaves and root bark of Tetracera alnifolia Willd. (Dilleniaceae) were investigated for anti-Mycobacterium tuberculosis activity using the MABA assay method. Anti- Mtb activity was determined against Mtb H37RvATCC 27294 at concentrations of 100- 0.390μg/mL. The hexane and chloroform extracts of the root bark of Tetracera alnifolia and the chloroform extract of Uvaria afzelli had anti- Mtb activity with MIC <100 μg/mL. Phytochemical screening for secondary metabolites revealed the presence of tannins, triterpenoid saponins, cardiac glycoside and alkaloids. The anti- Mtb activity demonstrated by the crude extracts is attributed to the presence of tannins and other secondary metabolites which are known to have strong antimicrobial activity. The results therefore support the local use of Uvaria afzelli and Tetracera alnifolia in the treatment of cough associated with tuberculosis and other microbial infections of the respiratory tract and suggest that these plants may be of therapeutic importance in the treatment of tuberculosis.