Botany
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Item Antibacterial activities of daldina concentrica(2011-01) Jonathan, S. G.; Olawuyi, O. J.; Popoola, O. O.; Aina, D. A.Activities of the distilled water, ethanolic and chloroform extracts of Daldina concentrica an ascomycetous fungus was investigated on Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Bacillus cereus and Staphylococcus aureus) using agar well diffusion method. Ethanolic extract of Daldina concentrica showed significantly antibacterial activity against all the test microorganisms except Bacilllus cereus (P ≤0.05). Staphylococcus aureus was the most sensitive organism to the extracts of this fungus with 17.0mm zone of inhibition with ethanol extract. It was also shown that chloroform extract of Daldinia concentrica possessed higher anti-bacterial activity against the five tested microorganisms. The effect of fresh tissues of the fungus on test bacteria indicated that Proteus mirabilis was the only sensitive organism with 5.0mm zone of inhibition while other bacteria were resistant. The implications of these findings were discussed.Item Studies on antimicrobial potentials of three ganoderma species(2010-05) Jonathan, S. G.; Awotona, F. EThe fruit bodies of three Ganoderma species namely G. lucidium, G. applanatum and G. australe were collected from the decaying logs within the University of Ibadan Botanical Gardens .Invitro antagonistic effect of the ethanol, methanol and distilled water extracts of these macro fungi were tested against some disease causing microorganisms. Both crude and pure extracts of these Ganoderma species exhibited various degree of inhibition against the test organisms. The widest inhibitory zone (20.3mm) were obtained with the crude methanolic extract of G. lucidium against Proteus mirabilis while the highest in-vitro antifungal activity (24.3mm) was observed in the crude ethanolic extracts of G. lucidium against Aspergillus niger. The lowest zone of inhibition (2.3mm) was demonstrated with the aqueous extract of G. australe against Escherichia coli and 2.7mm with purified extract of G. australe against Penicillum oxalium . The minimum inhibitory concentration (MIC) for the ethanol extract ranged between 1.7 and 5.0mg/ml for bacteria and between 2.0 and 6.0mg/ml for fungi. The implications of these findings were discussed.