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Item Evaluation of photoperiod sensitivity and adaptation in Kenaf (Hibiscus cannabinus) in some Nigerian agro-ecologies(Elsevier, 2012) Balogun, M. O.; Akande, S. R.; Raji, J. A.; Ogunbodede, B. A.Seven genotypes of Kenaf were grown in pots at controlled and natural photoperiod (PP) regimes in Ibadan, South-western Nigeria, to determine their PP sensitivities. Numbers of days to flowering and fibre yield were recorded. The same genotypes were grown on the field at Jos, Ibadan and Badeggi of high altitude, southern guinea savannah and forest agro-ecologies of Nigeria, respectively. Days to flowering, fibre and seed yields were recorded. Potted plants grown at natural PP flowered earliest, 54 days after planting (DAP) while they flowered at 118 DAP at 12 hours PP. IFEKEN DI400flowered 20 days later than other genotypes at both PP regimes, and was grouped as being relatively photo- insensitive. Fibre yield was significantly higher at natural than at 12 hours PP only in IFEKEN DI400, with highest mean yield of24.08g / plant. In the genotype x environment studies, IFEKEN DI400 was consistently latest to flower across locations with an average of 107 days. Mean fibre yield of 11,314.5kg/ha in Ibadan tripled that obtained in Badeggi and Jos. Genotype Tainung had the highest seed yield of 473.75g and 179.35g at Badeggi and Ibadan, respectively while the lowest seed yield 201.08g for Jos was recorded in genotype 8B. Badeggi is recommended for seed production while Ibadan is recommended for fibre production pending the availability of specifically adapted genotypes. The genotypes IFEKEN DI400, Tainung and 8B are candidate genotypes in breeding for high yield and adaptation to Nigerian agro-ecologies.Item Effects of plant growth regulators and explant types on callus formation in African yam bean (sphenostylis stenocarpa (hochst. Ex A. rich) harms)(0075-5192, 2009) Akande, S. R.; Balogun, M. O.; Ogunbodede, B. A.This study was conducted to investigate the effect of explant types and plant growth regulator regimes on callus induction in African yam bean, with a view to developing protocols for regeneration Leaf, stem and root explants of African yam bean were cultured in media containing different types and concentrations of plant grow& regulators (PGR). The explants were observed for callus formation after three months. The main effects of variety, explant and plant growth regulator regimes and their interactions were highly significant Highest callus formation was recorded in stem explant while the root explant had the lowest. The accession, SSSWN56 brown seed) had higher percentage callus formation than ssswN7s (grey seed). No callus was formed in hormone-free medium and the media containing indole acetic acid (IAA). One- hundred-percent callus formation was recorded for both accessions in the medium containing 1.5 mg/l each of kinetin and Naphthalene acetic acid (NAA). This medium is recommended for callus induction using stem explant and it will be useful in perfecting regeneration protocols for African yarn bean.Item Effects of plant growth regulators on callus, shoot and root formation in fluted pumpkin (telfaira occidentalis)(Academic Journals, 2007-02) Balogun, M. O.; Akande, S. R.; Ogunbodede, B. A.Root and stem explants of fluted pumpkin were cultured in medium containing different types and concentrations of plant growth regulators (PGRs). The explants were observed for callus, root and shoot formation parameters after four months. Differences among explants, plant growth regulators and their interaction were not significant for number of roots per plantlet. Callus formation was higher in stem than root explants. Stem explants formed more callus in medium containing naphthalene acetic acid than that containing indole acetic acid while the trend was opposite with root explants. Root explants did not form shoots, leaves or nodes in any of the PGR regimes, while a concentration of 1.5 mg/l benzylaminopurine (BAP) induced the highest numbers of shoots, nodes and leaves per stem explant. There was no callus, shoot, node and leaf formation by both explants when cultured in medium without PGRs while root formation was minimal.Item Variation in photo- and thermal- sensitivities among local, improved and exotic kenaf accessions in Nigeria(WFL Publishers, 2007) Balogun, M. O.; Raji, J. A.; Akande, S. R.; Ogunbodede, B. A.The response of two local, eleven improved and one exotic accessions of kenaf to daylength was determined by their increase in vegetative growth after flowering in 2004. Seven out of the fourteen accessions were planted every 4 months in 2005 to determine their sensitivity to natural variations in daylength, temperature, solar radiation and relative humidity in tenns of days to flower initiation, percentage gain in height after flowering and fibre yield. The plants took longer days to flower at longer daylength and higher temperature. Differences among accessions were significant for all traits in 2004. Days to flowering were more in December than in April and August, the number of days differing among accessions. Percentage gain in height after flowering differed with planting date only in the heat-tolerant accessions and was highest in December. Differences in fibre yield were not significant among accessions when planted in August, but differed significantly in April and December. On the average, fibre yield was highest in December and lowest in August. A grouping of the accessions based on their responses to daylength for both years of study was consistent. The implications of these findings in development of agronomically superior varieties and production of raw materials all round the year in Nigeria are discussed.Item Effects of plant growth regulators on indirect somatic embryogenesis in kenaf (hibiscus cannabinus )(The Genetics Society of Nigeria, 2006) Balogun, M. O.; Akande, S. R.; Ogunbodede, B. A.To develop protocols for regeneration of kenaf via indirect somatic embryogenesis, hypocotyl and cotyledon explants of two genotypes were cultured at different 2,4- dichlorophenoxyacetic acid and kinetin concentrations. The highest callus yields were obtained in medium containing both 0.5mg of kinetin and 0.1mg of 2, 4-dichlorophenoxyacetic acid per litre of medium. The calli were further cultured in twelve different media containing different plant growth regulators to induce somatic embryogenesis. Friable calli were mostly produced in one genotype (Ifeken400) and non-friable in the other (Ifeken400). Among the plant growth regulator regimes, media containing 3.0mg/I of kinetin and that containing 1.5mg/l each of kinetin and NAA did not support production of globular embryos in both varieties. The friable and non- friable caili were most responsive to kinetin and NAA respectively in terms of formation of torpedo embryos. The most advanced walking stick embryos were produced only in Ifeken400 in 1.5mg/l NAA. The importance of genotype and callus type in successful regeneration of kenaf by indirect somatic embryogenesis is discussed. Research is ongoing to achieve whole plantlet regeneration.Item Microproagation of fluted pumkin by enhanced axilary shoot formation(2002-05) Balogun, M. O.; Ajibade, S. R.; Ogunbodede, B. A.In a bid to develop protocols for micropropagation of fluted pumkin (Telfairia occidentalis), single node cuttings of two genotypes, Telfairia occidentalis white and Telfairia occidentalis orange (ToW-1 and ToR-1 respectively) were cuItured in Murashige and Skoog medium but with four different combinations of kinetin and naphthalene acetic acid concentrations. The highest number of nodes per plantlet was obtained in the medium containing l=litre2.0mg/l kinetin which indicates suitability for micropropagation purposes. There was however, lack of root formation: Premature senescence was also significantly predminant in ToR-1 relative to ToW1. Optimum medium for Telfairia micropropagation appears to be genotypeItem Development of high yielding, late maturing kenaf (hibiscus cannabinus) using gamma irradiation(Food and Agriculture Organization of the United Nations, 2009) Balogun, M. O.; Akande, S. R.; Raji, J. A.; Ogunbodede, B. A.; Agbaje, G. O.; Adeyeye, O. O.