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    Yam propagation using 'aeroponics' technology
    (2014) Maroya, N.; Balogun, M.; Asiedu, R.; Aighewi, B.; Kumar, L.; Augusto, J.
    Aims: To study yam propagation and seed yam tuber production in aeroponics system. Study Design: The experiment was conducted in a randomized complete block design and treatments were replicated three times. Place of Study: This experiment was carried out at the International Institute of Tropical Agriculture Headquarters at Ibadan in Nigeria. Methodology: The experiment tested fresh vine cuttings of five yam genotypes of two species in an aeroponics system. Three genotypes of Dioscorea rotundata (TDr 89/02475, TDr 89/02665 and TDr 95/18544) and two yam genotypes of D. alata (TDa 98/01176 and TDa 291) were evaluated. Results: Vines of both D. rotundata and D. alata rooted within 2 weeks in aeroponics system. The rooting of vine cuttings varied significantly among genotypes with a maximum of 98% for TDa 98/01176 and a minimum of 68% for TDr 89/02665. Mini-tubers harvested after 4 months of growth in aeroponics weighed between 0.2 and 2.7g. A second harvest 6 months later gave mini-tubers of up to 110g. The analysis of variance showed significant difference (P<0.05) among genotypes for rooting at 2 weeks after vine planting, number of plant surviving at 90 days after planting and percentage of plants with bulbils. The best genotypes were TDr 95/18544 and TDr 89/02665 for survival at 90 days after vine planting and percentage of plant producing bulbils in the aeroponics system respectively. Conclusion: This study revealed that yam genotypes performed differently in aeroponics system for vine rooting and production of mini-tubers and bulbils.
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    Status and prospects for improving yam seed systems using temporary immersion bioreactors
    (Academic Journals, 2014-04) Balogun, M. O.; Maroya, N.; Asiedu, R.
    Yam production is constrained by scarcity of clean seed, pests, diseases and low soil fertility in the informal seed system, which is still operational, causing up to 90% yield losses. Although meristem culture can be effective for producing healthy seed yam, its use is limited by slow rate of regeneration and propagation in conventional tissue cultures. In most crops tested, temporary immersion bioreactor systems (TIBs) increased propagation rates. To determine the potential of TIBs in improving the yam seed system, 23 databases were consulted and three returned a total of eight publications with only 2 for Dioscorea rotundata-cayenensis. Both plantlets and microtubers can be produced in TIBs, which will facilitate production of quality breeder, foundation and certified seeds and fast-track genetic improvement and the evolution of a formal from informal seed production system. Control of contamination, direct use of field explants, culture of micro-explants like immature embryos and anthers, increasing the size of microtubers produced and standardization for various economically important yam genotypes are knowledge gaps that require immediate research attention. No report has put a cost on yam TIBs, but it will be necessary to use cost-effective TIBs to encourage integration public-private partnerships into emerging formal seed system.