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Subject: search.filters.subject.Telfairia occidentalis

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    Effect of phytohormones and genotype on meristem and shoot tip culture of teifaira occidentalis hook F
    (Elewa Biosciences Journals, 2012) Adesoye, A. I.; Okooboh, G. O.; Akande, S. R.; Balogun, M. O.; Odu, B. O.
    Objectives: The aim of this work was to investigate the effect of plant growth regulators (PGR), genotype and explant type on in vitro shoot induction, elongation, and multiplication in Telfairia occidentalis. Methodology and Results: In this study attempts were made to induce shoots from meristem and shoot tip culture of Telfairia occidentalis Hook F. Shoot apical meristems with 1-2 leaf primordia and shoot tip explants were aseptically isolated and cultured on ½ N6 medium supplemented with different combinations of 2mg/l BAP, IAA and kinetin. Explants were derived from seedlings of two T. occidentalis genotypes grown in sterilized soils inside vials in the laboratory. Regeneration response were examined based on five parameters – callus formation, shoot length, number of shoots per explant, number of leaves per explant and number of nodes. Shoot induction, elongation and multiplication were most effectively promoted by the medium supplemented with 2.0 mg/lBAP + 2.0 mg/l IAA for shoot tip culture, while 2.0 mg/l BAP was most effective for shoot regeneration from meristem culture. Genotype I was significantly higher than Genotype II in shoot bud regeneration response (p<0.05). Culture of explants from shoot tip led to better shoot egeneration in comparison to explants from meristem. Callus formation/induction was also influenced by explants and media interaction. Conclusion and Application of Findings: In vitro shoot induction from meristem and shoot tip culture of T. occidentalis has been demonstrated. These in vitro culture procedures would be useful for developing uniform clones or micropropagation and could also form the basis for in vitro storage of explants and subsequent regeneration of plantlets after long term conservation in this species. The recalcitrant nature its seeds makes alternative means of genetic resources conservation very necessary. More importantly, meristem culture technique is useful for developing virus-free clones and avoids the limitations imposed by conventional mode of planting. Calli produced could also be excellent targets for genetic transformation and improvement of this species.
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    Effects of plant growth regulators on callus, shoot and root formation in fluted pumpkin (telfaira occidentalis)
    (Academic Journals, 2007-02) Balogun, M. O.; Akande, S. R.; Ogunbodede, B. A.
    Root and stem explants of fluted pumpkin were cultured in medium containing different types and concentrations of plant growth regulators (PGRs). The explants were observed for callus, root and shoot formation parameters after four months. Differences among explants, plant growth regulators and their interaction were not significant for number of roots per plantlet. Callus formation was higher in stem than root explants. Stem explants formed more callus in medium containing naphthalene acetic acid than that containing indole acetic acid while the trend was opposite with root explants. Root explants did not form shoots, leaves or nodes in any of the PGR regimes, while a concentration of 1.5 mg/l benzylaminopurine (BAP) induced the highest numbers of shoots, nodes and leaves per stem explant. There was no callus, shoot, node and leaf formation by both explants when cultured in medium without PGRs while root formation was minimal.
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    Sex differences in biochemical contents of telfaira occidentalis hook F.
    (WFL Publishers, 2006) Ajibade, S. R.; Balogun, M. O.; Afolabi, O. O.; Kupolati, M. D.
    In order to evolve ways of early sex identification in Telfairia, the biochemical contents of leaf, vine and root of male and female plants were determined in three genotypes. Female plants had significantly higher concentrations of protein and fat while the male plant was higher in fibre, as hand all the anti-nutrients. The concentration of trypsin inhibitor was most influential in distinguishing between the sexes especially for root and vines while the fibre content was most important for the leaf. Leaf fibre content is therefore recommended for sex identification since it can be easily determined at any stage of plant development without destroying the whole plant.