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Item Toxicity of calcium hypochlorite on fingerlings of Clarias gariepinus (Burchell, 1822)(Faculty of Agriculture and Forestry, University of Ibadan, 2014) Kolawole, A. S; Olukunle, O.The toxicity of calcium hypochlorite (containing 65% chlorine disinfectant) was investigated on fingerlings of Clarias gariepinus. Fish of mean weight of 5.5 ± 0.8g and total length of 7.8 ± 0.5cm were exposed to various concentrations (0.00, 0.011, 0.033, 0055 and 0.077 mg/I) of calcium hypochlorite resulting in residual chlorine of(0.00, 0.002, 0.006, 0.010 and 0.014 mg/l respectively) for 12, 24, 48 and 96h, adopting a static bioassay technique. The water quality, tolerance and behavioural responses, haematological and histopathological parameters of fingerlings of Clarias gariepinus were monitored and measured using standard methods. Blood samples were obtained from the caudal circulation and used for the measurement of Packed Cell Volume (PCV), Haemogolobin level (Hb), Red and white Blood cell count. Erratic swimming, excited competitive movements and gasping for air at the surface were the behavioural changes observed with increase in time of exposure and concentration of the chemical. The water quality parameters were measured during and after the experiment. Significant differences were observed. The PCV showed significant (P<0.05) decrease in values between 25% and 20% in all concentrations compared to the control value of 29% at 96h exposure. Haemoglobin levels were similarly significantly (P<0.05) lowered from the control value of 8.3g/dL to 6.7g/dL in the varying concentrations with increase in time of exposure. Deteriorating changes were observed in the liver, gill and brain of fingerlings of C. gariepinus exposed to calcium hypochlorite with increase in both concentration and time of exposure. It is therefore concluded that treated municipal water supply which usually contains residual chlorine of 0.20ppm is not suitable for aquaculture. It is recommended that the water should be exposed to light between 24 to 48 hours in order to allow for the evaporation of the residual chlorine present in it if it must be used at all. Calcium hypochlorite should not be used indiscriminately in aquaculture systems.Item Haematological changes of Clarias gariepinus juveniles fed different dietary lipid(Fisheries Society of Nigeria, 2012) Oshoke, J. O.; Olukunle, O. A.; Ajayi, A. I.; Dasuki, Saulawa, L. A. A.Twelve weeks feeding trial was conducted to determine the effects of different dietary lipid sources on the hematological changed in Clarias gariepinus juveniles, 6 iso-nitroginous diets were formulated at 45% CP and fed to triplicate groups of 15 juveniles. The feed contained (Palm Seed Oil (PSO), Ugwu Seed (USO), Soya Bean Oil (SBO), Almond Seed Oil (ASO), Mixture Of All the vegetable oil + the fish oil (MOA) and Cod Liver Oil (CLO) which is the control. The oils were added at 5% inclusion level respectively. Fish of mean weight 22.83+ 0.30g were fed these experimental diets in triplicate groups. The hematological analyses of fish showed that the red blood cell, white blood cell, Erythrocyte sedimentation rate (ESR), Mean cell volume (MCV), Mean cell Haemoglobin (MCH) and packed cell volume were not significantly different (p<0.05), but haemoglobin concentration and Mean cell haemologlobin concentration (MCHC) were significantly different (p>0.05). The present study showed that PSO, USO, ASO, SBO and MOA can effectively replace cod liver oil without compromising the health of African catfish, Clarias gariepinus.Item Hormonal and haematological responses of Clarias gariepinus (Burchell 1822) to nitrite toxicity(Medwell Journals, 2007) Ajani, F.; Olukunle, O. A.; Agbede, S. A.Study on hormonal and haematological responses of Clarias gariepinus to nitrite toxicity was carried out to know the magnitude of the effects of this stressor on fish physiology. Haematological responses of C. gariepinus to sub-lethal levels of nitrite (0.1 g L-1) at different exposure h (0, 6, 24, 48, 72 and 96 h) were carried out. Blood samples of C. gariepinus were collected at each exposure h and evaluated for primary and secondary stress indicators. Data obtained were subjected to simple descriptive analysis, analysis of variance and Pearson Product Moment correlation analysis. When exposed to nitrite, there was progressive increase in plasma cortisol level of C. gariepinus from 0 h (101.00± 0.10 ng dL-1) to 48 h (161.00± 1.20 ng dL-1), this later decreased at 72 h (107.00±1.60 ng dL-1), it increased in 96 h to 136.00±1.00 ng dL-1. PCV and Hb were elevated after 6 h of exposure and decreased significantly (p<0.05) at 48, 72 and 96 h of exposure in fish exposed to sub-lethal level of nitrite. From the value of 3.69±0.01 106/l recorded at 0 h, RBC level decreased significantly (p<0.05) at 6 h, this was followed by significant increase (p<0.05) at 24 h. The RBC value later decreased gradually to 2.20±0.56 106 L-1 at 96 h. The study shows that hormonal and haematology balances of C. gariepinus was affected on short term exposure to nitrite toxicity.Item The nutritional value of cow blood meal and its effects on growth performance, haematology and plasma enzymes of hybrid catfish(2002) Olukunle, O. A.; Ogunsanmi, A. O.; Taiwo, V. O.; Samuel, A. A.The nutritional potential of sun-dried cow blood meal (CBM) was investigated for its effect on growth performance, haematology and plasma enzyme activities in hybrid catfish fingerlings (Clarias gariepinus Male x Heternbrunchus longifilis Female), in an assay that lasted for twelve weeks. Six graded diets containing 0, 5, 10, 15, 20 and 25 percent of CBM were compounded. Ten fingerlings (mean weight 4.23+0.64g) per tank were reared under laboratory conditions in eighteen plastic tanks, each containing 12 liters of water. The fingerlings were fed 3% of their body weight daily, with bimonthly adjustments. At the end of the 84-day experiment, growth performance parameters such as weight gains, specific growth rate (SGR) and feed conversion ratio (FCR) were determined. Blood samples were pooled from each treatment on days 0, 42 and 84 for haematotogical and plasma enzyme analyses. The specific growth rate and the feed conversion ratio were best in the fish fed the control (0% sun-dried CBM) diet and poorest in the fish fed 25% CBM diet inclusion. There were no significant differences (P>0.05) between mean daily weight gains, specific growth rate and feed conversion ratios of fish fed diet 2 (5% CBM) and the control diet. The higher the inclusion level of the sun-dried CBM in the experimental diets, the poorer the nutrient utilization and growth performance. Hematological and pIasma enzyme analyses revealed progressively severe macrocytic normochromic, anaemia, leucocytosis and high enzymatic activities of plasma alanine aminotransferase (ALT) and-aspartate aminotransferase (AST), gamma-glutamyl transferase (GGT) and alkaline phosphatase (ALP) in fish fed increasing levels of sun-dried CBM. These enzymatic changes imply liver, kidney and/or cardiac damage, and opportunistic systemic bacterial infection. It can be inferred that for optimum growth performance, best nutrient utilization and unimpaired haematological and plasma enzyme activities in hybrid Clarias, a 5 % level of sun-dried CBM inclusion in their diet is recommended.Item A comparative assessment of growth performance, haematology and serum biochemistry of hybrid catfish fed non-conventional plant proteins in homestead concrete tanks(2000) Olukunle, O.; Taiwo, V. O.; Adejinmi, J. O.An 84-day experiment was carried out to evaluate the potentials of three non-conventional plant proteins groundnut cake (GNC), soyebean cake (SBC) and sesame seed cake (SSC) singly and combined in the diets of hybrid catfish grown in homestead concrete tanks. Triplicate groups of 30 juveniles average weight of 26.9+ 0.18g were fed four diets which were formulated to contain 30% crude protein with 25% of the GNC (diet 1) component replaced by SBC (diet 2), SSC (diet 3) and a combination of the three in diet 4. All the diets contained an equal amount of animal protein (25.43g). At the end of 84 days of feeding, growth parameters, feed and carcass proximate analyses, mortality, haematology and serum biochemical parameters were measured. The results showed that the diets affected the growth of the juveniles significantly (P<0.05). The percentage weight gain of the fish fed diet 1 was 204% of the initial weight, while those fed diets 2, 3 and 4 were 405%, 465% and 445% respectively. Protein Efficiency Ratio (PER) was significantly higher (P <0.05) in diets 2, 3 and 4 than in diet 1, with diet 4 having the highest PER. The percent specific growth rate (SGR) recorded for each diet was not significantly different (P>0.05) from each other. Percentage mortality were 22.2%, 13.3%, 5.6% and 3.3% in fish fed diets 3, 4, 2 and 1, respectively. There were no significant variations in haematological parameters of fish fed all the various diets, except for those on diet 3 which exhibited lymphopenia and heterophilia (P<0.05). Similarly, there were no significant variations in serum biochemical parameters, except for hyperfibrinogenaemia, hypoglyeaemia and significantly increased AST activities (P<0.05) in fish fed diet 3 and 4. There were significant increases in the serum levels of cholesterol and triglyceride in fish fed diets 2 (P<0.05), 3 and 4 (P<0.01). The findings in this study showed that even though SBC and SSC inclusions in fish diet resulted in better growth performance than GNC inclusion, the relatively high levels of crude fat in these plants proteins especially SSC, may in fact be injurious to fish by causing fat deposition in tissues, fatty hepatic and renal damage, artherosclerosis and coronary heart disease both of which will predispose the fish to stress, innocuous disease and mortality.Item Chemical analysis and nutritional assessment of artocarpus heterophyllus lam. (jack fruit) defatted seeds used as addictive in feed for clarias gariepinus post juveniles(2013) Olaifa, F. E.; Ajayi, A. A.; Raji, I. O.A 49-day feeding trial was carried out with feeds supplemented with microgram quantities of the defatted seeds of Artocarpus heterophyllus in the diets of Clarias gariepinus at the post juveinile stage. Five diets at 40% crude protein were formulated containing 0, 15, 30, 45 and 60x106 μg DAH seed as additive. Each dietary treatment was replicated three times with 10 fish per replicate. Proximate composition of the defatted seed showed that it was rich in protein, carbohydrate and minerals. Fish on DAH–supplementd diets had better survival rates than the control. Haematology, plasma biochemistry and gross tissue examination were also carried out. No significant differences (p<0.05) were observed between the fish on DAH-containing treatments and the control. There might be need to further process and test defatted A. heterophyllus seeds as either probiotic or prebiotic on young fish for longer periods in order to take advantage of its rich supply of nutrientsItem The protective effect of walnut (tetracarpidium conophorum) leaf and onion (allium cepa) bulb residues on the experimental pseudomonas aeruginosa infection in clarias gariepinus juveniles(African Union, 2012) Bello, O. S.; Emikpe, B. O.; olaifa, F. E.The study assessed the effect of Walnut Leaf (WL) and Onion Bulb (OB) residues on disease resistance of Clarias gariepinus juveniles against infection with the bacteria pathogen Pseudomonas aeruginosa. Clarias gariepinus juveniles were fed with diets containing 0 (control), OB2 (0.5%), OB3 (1.0%), OB4 (1.5%), OB5 (2.0%), WL6 (0.5%), WL7 (1.0%), WL8 (1.5%) and WL9 (2.0%). Biochemical (serum total protein, albumin, globulin, albumin: globulin ratio) and haematological indices of the fish were investigated. Fish were exposed to 0.5ml of 107 Pseudomonas aeruginosa of 24h old culture with the percentage mortality and relative level of protection recorded for 4 weeks post – infection. The results demonstrated that the fish fed with treated diets showed increased in biochemical and haematological indices (P<0.05) compared with the control. The challenge infection showed an improvement from treated groups with percentage mortalities and relative level of protection highest in WL8 (3.33%, 90%) and OB2 (3.33%, 90%) and least (33.33%, 0%) in control respectively. The results suggest that walnut leaf residue at 1.5% inclusion for one month could be a potential, less expensive and promising dietary supplement that would positively affect growth, haematology and make C. gariepinus more resistant to Pseudomonas aeruginosa