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Author: search.filters.author.Ososanya, T. O.Subject: search.filters.subject.Haematology

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    Body weight changes, haematological and serum biochemical indices of West African (WAD) Dwarf rams fed ammonium sulphate fortified diets
    (2017) Ososanya, T. O.; Akinlade, A. T.
    Weight gain, haematology and serum biochemical parameters of sixteen (16) West African Dwarf (WAD) rams weighing 12.8+0,12 kg were assigned to diets containing 0g/kg, 2.5g / kg, 5.0g/kg and 7.5g/kg levels of ammonium sulphate ((NH4)2SO4) as T0, T2.5, T5.0 and T7.5, respectively. The animals were fed the diet and wilted guinea grass in a 60:40. Daily weight gain, feed conversion ratio, platelet, lymphocytes, neutrophil, cholesterol, Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Alkaline Phosphatase (ALP) values of the rams fed ammonium sulphate fortified diets were significantly (p<0.05) higher than the control. Haemoglobin concentration increased in ramsfed ammonium sulphate fortified diets from 14.00-14.68% compared to control. The white blood cell (x 10(6)/mm(3)) varied from T0 (4.3 I) to T7.5(5.15). The lowest packed cell volume (41.00%) was obtained for rams on control diet. Red blood cell (x 10(6)/mm(3))) ranged from T0 (11.86) to T7.5 (13.02) while alanine aminotransferase (ALT) ranged from 10.25 (T0) to 25.25 (T7.5) (x 10(6)µL). The study revealed that inclusion of ammonium sulphate fortified diets of WAD rams up to 7.5g/kg had no deleterious effects on weight gain, haematological and serum indices of WAD rams
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    Growth performance and blood profile of gestating wad ewes fed dietary supplementation of ammonium sulphate
    (The Nigerian Society for Animal Production, 2016) Ososanya, T. O.; Inyang, U. A.; Shehu, S. A.
    The concern raised as a result of antibiotic usage in the livestock industry has necessitated the need for alternative feed additives such as ammonium sulphate. Hence, sixteen West African Dwarf (WAD) ewes averaging 24 kg in weight and aged eighteen months were used to determine the growth performance and blood chemistry of ammonium sulphate supplemented diets. The animals were divided into four groups of experimented diets consisting: D1 (0 %) (Control diet), D2 (0.25 %), D3 (0.50 %) and D4 (0.75 %) of ammonium sulphate (AMS) inclusion levels and were randomly allotted in a completely randomized design. They were also synchronized with prostaglandin F(2α) and naturally mated with four (4) rams of proven fertility when they came on heat. Parameters determined were feed intake, dry matter intake (DMT), gestating weights and gains, and blood haematology and serum biochemistry such as white blood cells (WBC), red blood cell (RBC), packed cell volume (PCV), haemoglobin (HGB), albumin (A), globulin (G), total protein (TP), cholesterol (CHOP), blood urea nitrogen, creatinine (C), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP). The results showed that supplementing AMS in ewe diets generally enhanced (p < 0.05) final weight (Kg), gestational weight gain (Kg) and average daily weight gain (g). The values ranged from 32.00-45.25 Kg; 8.25 - 22.25 Kg; 207.79 - 267.86 g/d respectively. The feed conversion ratio improved with increasing levels of AMS with animats on D4 recording the least (0.20). Animals on Dl recorded the highest values for PCV, HGB, and RBC while those on D3 had the least. The values were 44.25 and 41.00 %; 14.68 and 13.40 g/dl; and 13.02 and 11.86 x 10(6)/mm(3) respectively. Animals on D4 recorded the highest values for WBC (5150.50 x 10(6)/mm(3) ). Serum biochemical responses of pregnant sheep indicated significant differences (p < 0.05) of AMS supplementation on TP, A/G ratio, ALP and creatinine values. Animals on Dl recorded the highest values for TP, A/G ratio; ALT, BUN and C white those on D2 were least except jar ALT where D3 was least in ALT. The values were 8.68 and 8.15 g/dl; 0.80 and 0.65; 289 and 235.50 I.U./L; 10.75 and 9.75 mg/dl; 1.48 and 1.08 mg/dl respectively. Animals on D2 recorded the highest values for G, ALP and CHOL (4.80 mg/dl; 25.25 1. U/L; and 63.00 mg/dl respectively). Inclusion of AMS improved conversion ratio thereby improving the performance of ewes and thus proved that it could be tolerated up to 0.75 % supplementation in diets for ewes.
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    Influence of water-washed neem fruits (azardirachta indica A. juss) on heamatology and serum biochemical indices of West African dwarf sheep
    (Science Domain, 2014) Ososanya, T. O.; Adewumi, M. K.; Faniyi, T. O.
    Neem is a fast growing tree that thrives well in all parts of Nigeria. The seeds are readily available because the plant is used to control desertification. A 4 - week feeding trial was conducted to investigate the effect of supplementation of water - washed neem fruit (Azardirachta indica A.juss) in diets on haematological and serum biochemical indices of West African Dwarf (WAD) ewes. Three diets were formulated to contain water-washed neem fruit at graded levels of inclusion; 0% (control), 5% and 10%. Twelve 10 month old WAD ewes were randomly assigned to 3 dietary treatments. Each treatment had 4 replicates. Variations in values of haematological and serum biochemical parameters of WAD ewes fed experimental diets were determined. Some haematological parameters (RBC and Hb counts) measured were significantly (p<0.05) different while PCV, WBC, some differential counts and serum biochemical parameters were not significantly different among the treatments. However, urea, creatinine, glucose and alkaline phosphatase levels in serum of WAD ewes showed significant (p<0.05) differences, while, other serum biochemical indices measured were not significant. Although, the result of this study showed that the inclusion of water - washed neem fruit at 10% depressed some heamatological parameters, but showed no adverse effect on the ewes. However, all the values obtained for serum biochemical indices were within the normal physiological range except for alkaline phosphatase. Therefore, water - washed neem fruit can be included in the diet of WAD ewes at 10% without any deleterious effect.
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    Body weight changes, heamatological and serum biochemical properties of West African dwarf rams fed ginger fortified diets
    (2016) Ososanya, T. O.
    The effect of feeding diets fortified with 0, 0.5, 1.0 and 1.5% ginger powder on body weight changes, haematological and serum biochemical properties and described as T1, T2, T3 and T4 respectively was determined using sixteen 12-18 months old WAD rams weighing 15.86 + 0.03kg. The animals were grouped into 4 treatments in a completely randomized design with 4 animals per treatment and fed the experimental diets for 28 days. Daily weight gain, feed intake and feed conversion ratio were monitored. Blood samples were collected and analyzed for haematological indices {Packed Cell Volume (PCV, %), Red Blood Cell (RBC, 10(6)/mm(3) and White Blood Cell (WBC, 10(3)/mm(3)} and biochemical parameters {Total protein (TP, g/dl), Blood urea nitrogen (BUN, mg/dl), Aspartate Transferase (AST, I.U. /I) and Alanine Transaminase (ALT, I.U. /1)}. PCV ranged from 33.75 (T4) to 39.00 (T2) and the values were within the recommended PCV value for WAD ram. Similarly, WBC values for rams on T4 (10.37) and T3 (8.80) were significantly higher than T2 (6.05) and Tl (4.87). Total protein and BUN increased linearly across the treatments. The values for AST and ALP ranged from 72.10 (T2) to 147.00 (T3) and 238.00 (T4) to 294.00 (T2) respectively. Consequently, it can be concluded that 1.5% fortification of ginger powder promoted body weight gain and had no deleterious effect on the haematological and serum biochemical profile of WAD rams.
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    Haematology parameters of West African dwarf rams fed velvet bean (mucuna pruriens) ensiled with whole maize stover
    (Animal Science Association of Nigeria, 2015) Alabi, B. O.; Ososanya, T. O.
    The haematological characteristics of West African Dwarf (WAD) Rams fed velvet bean (Mucuna pruriens) foliage ensiled with whole maize were determined. Twenty (20) growing rams aged 10-15 months with mean weight ranging between 11.75-12.00kg were randomly assigned to four dietary treatments comprising five animals per dietary treatment in a completely randomized design (CRD). Treatment 1 contained 100% ensiled whole maize, Treatment 2 contained 75% whole maize + 25% Mucunapruriensfoliage silage, Treatment 3 contained 50% whole maize + 50% Mucunapruriensfoliage silage, Treatment 4 contained 25%> whole maize + 75% Mucunapruriensfoliage silage respectively. Each animal receive each diet at 5% body weight for 105 days. Blood was collected through jugular vein and put into well lebelled bottle containing anticoagulant ethylene diaminetetracetic acid (EDTA). The blood samples were analyzed for Red blood cell (RBC) count, Parked cell volume (PCV), haemoglobin (Hb), white blood cell (WBC) count, neutrophils, lymphocytes, eosinophils and monocytes. Significant differences (P<0.05) were obtained in haemoglobin and neutrophil which range from 6.11-9.87g/dl and 35.28 - 41.23% respectively while other parameters investigated showed no significant (P>0.05) differences. The nutritional health status of the rams can be enhanced when fed Mucunapruriensfoliage and whole maize silage but best result could be obtained when fed silage containing 75% whole maize and 25% Mucunapruriens.