scholarly works
Permanent URI for this collectionhttps://repository.ui.edu.ng/handle/123456789/452
Browse
2 results
Search Results
Item Genetic Variations in Thomas’s Rope Squirrel (Funisciurus anerythrus) and Gambian Sun Squirrel (Heliosciurus gambianus) Ibadan, Nigeria, Using Allozyme(2020) Coker, O.M.; Osaiyuwu, O.H.; Isong, O.M.Thomas’s Rope Squirrel (Funisciurus anerythrus) and Gambian Sun Squirrel (Heliosciurus gambianus) are two of the eight squirrel species found in Nigeria with overlapping habitats in Southwestern Nigeria. Squirrels are involved in pollination, seed dispersal, vectors of human and domestic animal diseases as well as crop and household pests. These species and the crucial ecosystem services they render are threatened by habitat destruction, human encroachment and the fact that they are being used as a source of protein in Nigeria. Their conservation would be enhanced by availability of accurate genetic information which are scarce for these species. This study seeks to provide baseline data on genetic variation at three allozyme loci in the two species in University of Ibadan, Ibadan, Nigeria. Twenty-nine individual squirrels including eighteen F. anerythrus species and eleven H. gambianus were used in this study. These were obtained from the wild within the University of Ibadan. Observed and expected heterozygosity (Ho and He), Hardy Weinberg equilibrium (HWE), genetic distance between the species, and inbreeding coefficients were performed using POPGENE and Tools for Population Genetic Analyses (TFPGA). In F. anerythrus, Ho and He were 0.6092 and 0.5635, respectively, while, for H. gambianus, Ho and He were 0.6242 and 0.5745, respectively. There were no deviations from HWE in all the tested loci (p>0.05). Nei genetic distance between the species was 0.0070 and the populations showed fairly high level of outbreeding (Fst = 0.0029 ± 0.0017). More robust genetic markers would be required to further ascertain the genetic status of the species.Item Genetic structure of indigenous sheep breeds in Nigeria based on electrophoretic polymorphous systems of transferrin and haemoglobin(2018) Osaiyuwu, O.H.; Salako, E.A.The study used 100 indigenous sheep comprising 25 Balami, 25 Uda, 25 Yankassa and 25 West African Dwarf breeds reared extensively. The blood samples were taken from Vena Jugularis, processed according to standard procedure and transferrin and haemoglobin examined using cellulose acetate electrophoresis. The observed allele frequencies and genotypes (%) were tested with Hardy-Weinberg’sEquilibrium (χ2). Seven alleles TfA, TfB, TfC, TfD, TfE, TfGand TfPcontrolling 23 genotypes were observed at the transferrin locus while two haemoglobin alleles (HbAand HbB) controlling three phenotypes HbAA, HbAB and HbBB were detected. Except for the West African Dwarf sheep, all the samples indicated genetic equilibrium revealed by significant difference between observed and expected genotypes at both loci. The observed significant difference between the frequencies of alleles and genotypes at the two studied loci in the West African Dwarf sheep can be used as a source of genetic diversity during selection for improvement. The phylogenetic analysis as viewed by the tree topology suggests that the Balami and Uda may have had the same migration route or may have been the same breed which hadonly just recently branched off through adaptive. Also, the West African Dwarf sheep may have been the first to branch off the path of migration and thus had more clearly defined migration route or origin.