scholarly works
Permanent URI for this collectionhttps://repository.ui.edu.ng/handle/123456789/394
Browse
2 results
Search Results
Item In-Vitro Susceptibility of Mycobacterium Tuberculosis to Extracts of Uvaria Afzelli Scott Elliot and Tetracera Alnifolia Willd(2011-01) Lawal, T. O.; Adeniyi, B. A.; Wan, B.; Franzblau, S. G.; Mahady, G. B.Tuberculosis is a global burden with one –third of the world’s population infected with the pathogen Mycobacterium tuberculosis and an annual 2 million deaths from the disease. This high incidence of infection and the increased rate of resistant strains of the organism (MDR- and XDR- TB) have called for an urgent need to develop new anti-tuberculosis drugs from plants. The crude extract of Uvaria afzelli Scott Elliot (Annonaceae) root bark, and leaves and root bark of Tetracera alnifolia Willd. (Dilleniaceae) were investigated for anti-Mycobacterium tuberculosis activity using the MABA assay method. Anti- Mtb activity was determined against Mtb H37RvATCC 27294 at concentrations of 100- 0.390μg/mL. The hexane and chloroform extracts of the root bark of Tetracera alnifolia and the chloroform extract of Uvaria afzelli had anti- Mtb activity with MIC <100 μg/mL. Phytochemical screening for secondary metabolites revealed the presence of tannins, triterpenoid saponins, cardiac glycoside and alkaloids. The anti- Mtb activity demonstrated by the crude extracts is attributed to the presence of tannins and other secondary metabolites which are known to have strong antimicrobial activity. The results therefore support the local use of Uvaria afzelli and Tetracera alnifolia in the treatment of cough associated with tuberculosis and other microbial infections of the respiratory tract and suggest that these plants may be of therapeutic importance in the treatment of tuberculosis.Item In vitro anti-mycobacteria sensitivity and Kill-kinetics of allium ascalonicum L. (whole plant) on nontuberculous mycobacteria species(2014-05) Igbokwe, C. O.; Lawal, T. O.; Adeniyi, B. A.Allium ascalonicum L. (Shallot) was one of the herbs repeatedly identified from the result of our ethnobotanical survey for the treatment of tuberculosis and nontuberculous mycobacteria diseases. It has been reported to show inhibitory potentials against several pathogens. This plant is also known to form part of the diet of many people across the world. In the quest for a more active and body-friendly therapeutic agents, extracts of Allium ascalonicum (whole plant) were screened against four nontuberculous mycobacteria species namely, Mycobacterium fortuitum ATCC 684, Mycobacterium smegmatis ATCC 19420, Mycobacterium abscessus and Mycobacterium phlei ATCC 19240. In vitro susceptibilities testing was done using agar diffusion method with the concentrations of extracts ranging between 25 to 200 mg/mL. The minimum inhibitory concentration (MIC) was determined by agar dilution methods while the kill or bactericidal kinetics was measured by viable counting technique. Methanolic extract demonstated significant inhibitory potentials against three of the organisms at the test concentrations with a minimum inhibitory concentration (MIC) of 100 mg/mL, and 72-98 % kill of susceptible organisms in 24 hours. This significant activity may be due to the presence and right combination of the secondary metabolites in the plant such as alkaloids, flavonoids saponins, cardiac glycosides and essential oil, as revealed from our phytochemical screening. This study therefore confirms the scientific bases and justifies the use of Allium ascalonicum L. in traditional medicine practice in Nigeria and other parts of the world; and encourages its consumption as a natural prophylaxis against tuberculosis and nontuberculous mycobacteria diseases.