Scholarly works in Pharmaceutical Microbiology
Permanent URI for this collectionhttps://repository.ui.edu.ng/handle/123456789/394
Browse
4 results
Search Results
Item Antibacterial activity of methanolic extract of Garcinia kola (Heckel) seed and standard antibiotics(African Society for Clinical Microbiology, 2006) Adeleke, O. E.; Ojo, O. P.; Idowu, P. A.The methanolic extract of Garcinia kola (Heckel) seeds and eight standard antibiotics were tested in-vitro for comparative activity against 10 isolates of each of six bacterial species: Escherichia coff. Staphylococcus aureus, Staphylococcus albus. Streptococcus pyogenes, streptococcus pneumoniae and Pseudomonas aeruginosa, all from throat infections. The methanolic extract exerted activity against all the bacteria tested almost in similar manner as gentamicin. Of the remaining seven standard antibiotics, chloramphenicol, erythromycin, and tetracycline showed activity against one organism or the other. Remarkably, augmentin, cloxaciilin and cotrimoxazole had no activity against any of the bacterial isolates. Amoxycillin was able to affect only 2 of die 8 isolates of Esch. coli. This has lent credence to the ethnopharmaceutical claims of the curative effect of raw-chewed Garcinia kola seeds on throat infections as well as highlighting the bacterial resistance to standard antibiotics, particularly, the ß-lactams.Item Antimicrobial activity of the crude extracts and isolated fractions of Garcinia kola Heckel stem bark(Science Association of Nigeria, 2006) Idowu, P. A.; Moody, J. O.; Odelola, H. A.The stem bark of Garcinia kola, commonly used for various ailments in traditional medicine in Nigeria was examined for antimicrobial activity. Crude chloroform and methanolic extracts and fractions isolated from the chloroform extract using column chromatography were screened for in-vitro antibacterial and antifungal activities. As tested using agar cup diffusion method on Staphylococcus aureus (NCTC 6571), Escherichia coli (NCTC 9001), Pseudomonas aeruginosa (NCTC 6750), Bacillus cereus (Lab. Stock), Candida albicans .(NCTC 7534) and Aspergillus niger (Lab. Stock), the crude extracts showed good activity at 10 and 20mg/ml respectively. The 14 fractions isolated from the column chromatography also showed varying but significant degree of activity on most of the tested organisms at lOOpg/ml. The study has shown that G. Kola bark has antimicrobial activities that justify its uses in traditional medicine in Nigeria as anti-infective agent. Also, the fact that most fractions showed antimicrobial activity is an indication that G. Kola bark has many antimicrobial constituents which are different but closely related.Item Anticancer and antibacterial activities of solanum aethiopicum L., solanum macrocarpon L. and garcinia kola heckel(2021-05) Oluremi, B. B.; Oloche, J. J.; Aden, A. J.Cancer and infectious diseases are among the top ten leading causes of death in developing countries. The discovery and development of newer agents for combating these global health problems cannot be overemphasized. This study was designed to investigate the activities of extracts of Solanum aethiopicum, Solanum macrocarpon and Garcinia kola on cancer and antibacterial pathogens. The Agar well diffusion method was used to evaluate antibacterial activity of the extracts. In vitro anticancer activity against cervical cancer (HeLa), breast cancer (MCF-7) and epidermoid carcinoma of the larynx (HEp-2) was done by microscopic evaluation of morphology and by determination of 50% cytotoxic concentrations (CC50). The CC50 of ethyl acetate extracts of Solanum aethiopicum and Solanum macrocarpon against MCF-7 were 38.79 μg/mL and 41.30 μg/mL respectively, and were significantly lower (p < 0.05) compared to the CC50 of the other extracts. Dichloromethane extract of Garcinia kola was significantly cytotoxic (p<0.05) on cervical cancer (CC50 = 38.13 μg/mL) compared to DCM extracts from Solanum aethiopicum and Solanum macrocarpon. The minimum concentration of methanol extracts of Solanum aethiopicum, Solanum macrocarpon and Garcinia kola that inhibited the growth of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae was 1.56 mg/mL. Solanum aethiopicum, Solanum macrocarpon and Garcinia kola extracts demonstrated significant (p < 0.05) anticancer and antibacterial activities and as such are promising sources of novel plant-derived anticancer and antibacterial drugs.Item Serum protein binding and the antimicrobial activities of garcinia kola, kola acuminata and kola nitida seed extracts on the agents of respiratory tract infections(West African Society for Pharmacology, 2009) Adeleke, O. E.; Alabi, O. S.; Coker M. E.The kinetics of bactericidal activity of the methanolic extracts of powdered seeds of Garcinia kola (Heckel), Kola acuminata and Kola nitida obtained by soxhlet extraction, were studied using two bacterial sensitive strains of Staphylococcus aureus and Pseudomonas aeruginosa. The study showed that the three extracts at the concentrations used against the two test organisms, Staphylococcus aureus OOUTH206 (0.5mg/ml for Garcinia kola, 4mg/ml for Kola acuminata and Kola nitida) and Pseudomonas aeruginosa UCH189 (8mg/ml for Garcinia kola, 15mg/mi for Kola acuminata and Kola nitida); had activity on the two microorganisms. There was a gradual reduction in the number of viable microbial counts as evident in the reduction in the cell number at the different time intervals. Similar procedure was carried out in the presence of 5% serum protein and it was observed that the activities of the various extracts were altered significantly indicating the effect of serum protein binding on the Constituents of Garcinia kola, Kola acuminata and Kola nitida seed extracts. The results were expressed in percentage viable count of the inoculum size and showed that the percentage viable count decreases with time in varying degrees. The reduction was more intense with G. kola extract than the other extracts on S. aureus as well as on P. aeruginosa towards the third and fourth hours. The effect of serum protein was seen to affect the activities of all the extracts in varying degrees as shown in the graphs plotted.