Wildlife & Fisheries management
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Item Potentials of walnut (tetracarpidium conophorum Mull. Arg) leaf and onions (allium cepa linn) bulb extracts as antimicrobial agents for fish(Academic Journals, 2013-05) Bello, O. S.; Olaifa, F. E.; Emikpe, B. O.; Ogunbanwo, S. T.The zone of inhibition varied with the bacteria and type of extract. The average diameter of inhibition zones was 10 ± 0.00 and 9 ± 0.02 mm for methanolic and ethanolic extracts of walnut leaves and onion bulbs, respectively. Staphylococcus aureus, B. subtilis and P. aeruginosa were most sensitive to the extracts. However, S. aureus was more sensitive to the extracts of walnut leaves and S. typhi was the least sensitive. Bacillus subtilis was more sensitive to the extracts of onion compared to E. coli which was the least sensitive. Minimum inhibitory concentration of walnut leaves and onion bulbs extracts on the bacteria tested were both 500 ìg/ml. The results indicated that walnut leaves and onion bulbs had antibacterial activity on the tested organisms and showed their prospects for their use in the treatment of fish diseasesItem The effect of walnut (tetracarpidium conophorum) leaf and onion (allium cepa) bulb residues on the tissue bacteriological changes of clarias gariepinus juveniles(2012) Bello, O. S.; Olaifa, F. E.; Emikpe, B. O.; Ogunbanwo, S. T.In this study, the effect of walnut leaf (WL) and onion bulb (OB) residues on tissue bacteriology of Clarias gariepinus juveniles by dietary intake was investigated. Nine experimental diets: control (0%), OB2 (0.5%), OB3 (1.0%), OB4 (1.5%), OB5 (2.0%), WL6 (0.5%), WL7 (1.0%), WL8 (1.5%) and WL9 (2.0%) were formulated and replicated thrice at 40% crude protein. Fish (mean weight 7.4±0.02g) were fed twice daily at 3% body weight for 12 weeks. Microbiological analyses of water and fish (skin, gill, intestine and liver) and organ index (liver, spleen, kidney and heart) were investigated. Data were analysed using descriptive statistics and ANOVA at p=0.05. Results of enterobacteriacea and total viable count from this study revealed that bacterial loads on the water and fish of the experimental tanks were more affected by A. cepa and T. conophorum than the control for 4, 8 and 12 weeks and were significantly different (P<0.05) from the control. The values decreased in treated groups as the levels of inclusion (0.5%, 1.0%, 1.5% and 2.0%) increased and as the months increased. Also, organ index showed that the liver, heart, kidney and spleen were not significantly increased in all the treated groups and the control. The results suggest that walnut leaf and onion bulb residues inclusion in the diet of Clarias gariepinus could be a potential, less expensive and promising dietary supplementation that would positively influence growth, reduce and prevent bacterial infections in fish culture.Item The effect of walnut (tetracarpidium conophorum) leaf and onion (allium cepa) bulb residues on the growth performance and nutrient utlization of clarias gariepinus juveniles(Canadian Center of Science and Education, 2012) Bello, O. S.; Olaifa, F. E.; Emikpe, B. O.; Ogunbanwo, S. T.Feeding trial were conducted in experimental tanks (50 x 34 x 27 cm) to assess the growth responses and nutrient utilization of Walnut Leaf (WL) and Onion Bulb (OB) residues in Clarias gariepinus. Nine experimental diets: control (0%), OB2 (0.5%), OB3 (1.0%), OB4 (1.5%), OB5 (2.0%), WL6 (0.5%), WL7 (1.0%), WL8 (1.5%) and WL9 (2.0%) were formulated and replicated thrice at 40% crude protein. Fish (mean weight 7.39±0.02 g and length 10.37±1.24 cm) were fed twice daily at 3% body weight for 12 weeks. Mean Weight Gain (MWG), Specific Growth Rate (SGR) and Feed Conversion Ratio (FCR) were measured. Temperature, dissolved oxygen and pH were determined using standard methods and economic analysis was investigated. Data were analyzed using descriptive statistics and ANOVA at p=0.05. Results showed that the Fish on OB and WL residue- based diets had higher growth than those on control diet but C. gariepinus fed WL8 had significantly higher MWG, SGR and FCR of 53.81±1.20 g, 1.09±0.11 g and 2.16±0.01 respectively of all treatments. The values of temperature, dissolved oxygen and pH were closely related and were still within the range for fish culture in the tropics. WL8 had higher profit index (1.40) and incidence of cost (0.032) and the least in OB5 (1.18) (0.039) respectively. These suggest that walnut leaf residue at 1.5% inclusion could be a potential, less expensive and promising dietary supplementation than onion bulb residue and control that would positively affect growth and water quality of Clarias gariepinus in aquaculture