Wildlife & Fisheries management
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Item Chemical analysis and nutritional assessment of artocarpus heterophyllus lam. (jack fruit) defatted seeds used as addictive in feed for clarias gariepinus post juveniles(2013) Olaifa, F. E.; Ajayi, A. A.; Raji, I. O.A 49-day feeding trial was carried out with feeds supplemented with microgram quantities of the defatted seeds of Artocarpus heterophyllus in the diets of Clarias gariepinus at the post juveinile stage. Five diets at 40% crude protein were formulated containing 0, 15, 30, 45 and 60x106 μg DAH seed as additive. Each dietary treatment was replicated three times with 10 fish per replicate. Proximate composition of the defatted seed showed that it was rich in protein, carbohydrate and minerals. Fish on DAH–supplementd diets had better survival rates than the control. Haematology, plasma biochemistry and gross tissue examination were also carried out. No significant differences (p<0.05) were observed between the fish on DAH-containing treatments and the control. There might be need to further process and test defatted A. heterophyllus seeds as either probiotic or prebiotic on young fish for longer periods in order to take advantage of its rich supply of nutrientsItem Studies on phyoremediation of copper using pteridium aquilinum (bracken fern) in the presence of biostimulants and bioassay using clarias gariepinus juveniles(Taylor and Francis, London, 2013) Olaifa, F. E.; Omekam, A. J.A study was carried out to evaluate the uptake of copper from water containing 10 mg/L copper by Pteridium aquilinum (bracken fern) and Clarias gariepinus in the presence of five plant growth stimulants: Nitrogen: phosphorus: potassium (15-15-15: an inorganic fertilizer), pig, cattle, poultry, and a mixture of pig/cattle manures. A plant growth stimulant differentiated each treatment. A 96-hour bioassay using C. gariepinus was carried out at the end of the experiment to test the efficacy of the clean up by P. aquilinum. The control experiment contained no copper or plant growth stimulant. Fish survival, uptake of copper by P. aquilinum, C. gariepinus, concentration of copper in water, hematology and histopathology of the fish were assessed. Higher concentrations of copper were reported in P. aquilinum than in water or C. gariepinus. Low fish mortality was reported with the highest being 20% in the cattle manure-containing treatment. Low fish mortality was reported with the highest being 20 percent in the cattle manure containing treatmentItem Chemical analysis and nutritional assessment of defatted garcinia mangostana seeds used as an addictive on the feed of fish (clarias gariepinus)(Global Journals Inc., 2013) Ajayi, I. A.; Olaifa, F. E.; Omoniyi, M. M.Chemical analysis and nutritional assessment of defatted Garcinia mangostana seeds (DGMS) were undertaken to determine its suitability as an additive at 0.00g, 18.00g, 36.00g, 54.00g and 72.00g inclusion levels in diets and performance of Clarias gariepinus post juveniles. Proximate analysis of DGMS showed that the defatted seeds were high in carbohydrate (71.00 ± 0.79%) but low in protein content (8.10 ± 0.22%). The mineral element analysis detected different minerals with potassium as the highest (270.00ppm). All the fish increased in weight and length significantly (p<0.05) above the initial values though no significant differences were observed among treatments at 49 days in all growth indices showing that the diets were similar in nutritional qualities and adequate for growth of fishItem The effect of walnut (tetracarpidium conophorum) leaf and onion (allium cepa) bulb residues on the tissue bacteriological changes of clarias gariepinus juveniles(2012) Bello, O. S.; Olaifa, F. E.; Emikpe, B. O.; Ogunbanwo, S. T.In this study, the effect of walnut leaf (WL) and onion bulb (OB) residues on tissue bacteriology of Clarias gariepinus juveniles by dietary intake was investigated. Nine experimental diets: control (0%), OB2 (0.5%), OB3 (1.0%), OB4 (1.5%), OB5 (2.0%), WL6 (0.5%), WL7 (1.0%), WL8 (1.5%) and WL9 (2.0%) were formulated and replicated thrice at 40% crude protein. Fish (mean weight 7.4±0.02g) were fed twice daily at 3% body weight for 12 weeks. Microbiological analyses of water and fish (skin, gill, intestine and liver) and organ index (liver, spleen, kidney and heart) were investigated. Data were analysed using descriptive statistics and ANOVA at p=0.05. Results of enterobacteriacea and total viable count from this study revealed that bacterial loads on the water and fish of the experimental tanks were more affected by A. cepa and T. conophorum than the control for 4, 8 and 12 weeks and were significantly different (P<0.05) from the control. The values decreased in treated groups as the levels of inclusion (0.5%, 1.0%, 1.5% and 2.0%) increased and as the months increased. Also, organ index showed that the liver, heart, kidney and spleen were not significantly increased in all the treated groups and the control. The results suggest that walnut leaf and onion bulb residues inclusion in the diet of Clarias gariepinus could be a potential, less expensive and promising dietary supplementation that would positively influence growth, reduce and prevent bacterial infections in fish culture.Item The effect of walnut (tetracarpidium conophorum) leaf and onion (allium cepa) bulb residues on the growth performance and nutrient utlization of clarias gariepinus juveniles(Canadian Center of Science and Education, 2012) Bello, O. S.; Olaifa, F. E.; Emikpe, B. O.; Ogunbanwo, S. T.Feeding trial were conducted in experimental tanks (50 x 34 x 27 cm) to assess the growth responses and nutrient utilization of Walnut Leaf (WL) and Onion Bulb (OB) residues in Clarias gariepinus. Nine experimental diets: control (0%), OB2 (0.5%), OB3 (1.0%), OB4 (1.5%), OB5 (2.0%), WL6 (0.5%), WL7 (1.0%), WL8 (1.5%) and WL9 (2.0%) were formulated and replicated thrice at 40% crude protein. Fish (mean weight 7.39±0.02 g and length 10.37±1.24 cm) were fed twice daily at 3% body weight for 12 weeks. Mean Weight Gain (MWG), Specific Growth Rate (SGR) and Feed Conversion Ratio (FCR) were measured. Temperature, dissolved oxygen and pH were determined using standard methods and economic analysis was investigated. Data were analyzed using descriptive statistics and ANOVA at p=0.05. Results showed that the Fish on OB and WL residue- based diets had higher growth than those on control diet but C. gariepinus fed WL8 had significantly higher MWG, SGR and FCR of 53.81±1.20 g, 1.09±0.11 g and 2.16±0.01 respectively of all treatments. The values of temperature, dissolved oxygen and pH were closely related and were still within the range for fish culture in the tropics. WL8 had higher profit index (1.40) and incidence of cost (0.032) and the least in OB5 (1.18) (0.039) respectively. These suggest that walnut leaf residue at 1.5% inclusion could be a potential, less expensive and promising dietary supplementation than onion bulb residue and control that would positively affect growth and water quality of Clarias gariepinus in aquacultureItem Heavy metal contamination of clarias gariepinus from a lake and fish farm in Ibadan, Nigeria(Ibadan Biomedical Communications Group, 2004) Olaifa, F. E.; Olaifa, A. K.; Adelaja, A. A.; Owolabi, A. G.Adult Clarias gariepinus (African Catfish) were purchased from Eleiyele Lake and Zartech fish farm in Ibadan. Water samples were also collected in February (dry season) and June (rainy season), 2002. Gill, bone, intestine, muscle and water samples were analyzed for five metals: manganese, copper, zinc, iron, and chromium by atomic absorption spectroscopy (AAS) in two separate experiments. In each case, two tissues were compared with the levels of the metals in water viz: Gill, bone, and water; intestine, muscle and water. Generally, lower concentrations of the metals were recorded in water than fish tissues. Higher concentrations of zinc than recommended by the Federal Environmental Protection Agency were recorded in the fish during the dry season. Iron was the dominant metal in the muscle while Chromium was the least. Significant differences (p<0.05) were recorded in copper and zinc concentrations in the muscle, intestine and water during the dry and rainy seasons .In gill, bones and water, significant differences (p<0.05) were only recorded for the two stations for copper during the rainy season and only zinc was significantly different (p<0.05) in the dry season. It was concluded that though the heavy metals of interest were present in measurable quantities there were still within safe limits for consumptionItem Toxic stress of lead on clarias gariepinus (African catfish) fingerlings(2003) Olaifa, F. E.; Olaifa, A. K.; Lewis, O. O.The toxic stress of lead on fish was tested employing a 96-hour bioassay test. The experimental fish used were Clarias gariepinus fingerlings. Lead in the form of lead chloride was used to prepare the stock solution. The concentrations of lead used for the experiment were 0, 1.8, 3.2, and 5.6, and10.0mg/l. The lethal concentration (LC50) was estimated using the logarithmic method and was found to be 0.6, 0.58 and 0.62mg/l for replicates1, 2 and 3 respectively.