Wildlife & Fisheries management

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    Investigation into the healing properties of walnut (tetracarpidium conophorum) leaf and onion (allium cepa) bulb residues in clarias gariepinus
    (2013-05) Bello, O. S.; Emikpe, B. O.; olaifa, F. E.
    The study evaluated the effect of dietary inclusion levels of walnut leaf (WL) and onion bulb (OB) residues on dermal wound healing of Clarias gariepinus. The experiment was carried out in 9 experimental tanks (1.8 m x 2 m x 1.2 m) for 14 days. Nine experimental diets were formulated at 40% crude protein representing the following inclusion levels: Control (0%), OB2 (0.5%), OB3 (1.0%), OB4 (1.5%), OB5 (2.0%), WL6 (0.5%), WL7 (1.0%), WL8 (1.5%) and WL9 (2.0%). Fish (mean weight of 1kg) were fed twice daily at 3% body weight. Percentage healing and daily healing rate on lateral and caudal parts of the male and female Clarias gariepinus were investigated at 0, 7 and 14 days. Data were analysed using descriptive statistics and ANOVA at P < 0.05. The results demonstrated that the fish fed with walnut leaf and onion bulb residues showed better performance than the control. Dermal wound healing of Clarias gariepinus was better on lateral and caudal parts (100%, 14.29; 100%, 14.29) in WL 8 compared to the control (98%, 14.00; 80%, 11.43) respectively. The results obtained showed that the male Clarias gariepinus had better healing compared to the female (100%, 14.29; 82%, 11.71), respectively. Diets with walnut leaf and onion bulb residues had wound healing properties and they could be use in fish farming. Also, the inclusion of 1.5% of walnut leaf residue in fish feed promotes wound healing in Clarias gariepinus
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    The protective effect of walnut (tetracarpidium conophorum) leaf and onion (allium cepa) bulb residues on the experimental pseudomonas aeruginosa infection in clarias gariepinus juveniles
    (African Union, 2012) Bello, O. S.; Emikpe, B. O.; olaifa, F. E.
    The study assessed the effect of Walnut Leaf (WL) and Onion Bulb (OB) residues on disease resistance of Clarias gariepinus juveniles against infection with the bacteria pathogen Pseudomonas aeruginosa. Clarias gariepinus juveniles were fed with diets containing 0 (control), OB2 (0.5%), OB3 (1.0%), OB4 (1.5%), OB5 (2.0%), WL6 (0.5%), WL7 (1.0%), WL8 (1.5%) and WL9 (2.0%). Biochemical (serum total protein, albumin, globulin, albumin: globulin ratio) and haematological indices of the fish were investigated. Fish were exposed to 0.5ml of 107 Pseudomonas aeruginosa of 24h old culture with the percentage mortality and relative level of protection recorded for 4 weeks post – infection. The results demonstrated that the fish fed with treated diets showed increased in biochemical and haematological indices (P<0.05) compared with the control. The challenge infection showed an improvement from treated groups with percentage mortalities and relative level of protection highest in WL8 (3.33%, 90%) and OB2 (3.33%, 90%) and least (33.33%, 0%) in control respectively. The results suggest that walnut leaf residue at 1.5% inclusion for one month could be a potential, less expensive and promising dietary supplement that would positively affect growth, haematology and make C. gariepinus more resistant to Pseudomonas aeruginosa