FACULTY OF VETERINARY MEDICINE
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Item Pathogenicity of Pseudomonas aeruginosa in reciprocal hybrids of Clarias gariepinus and Heterobranchus bidorsalis(University of Ibadan, Faculty of Veterinary Medicine, 2022) Obisesan O. M.; Oladosu G. A.; Adelakun, O. D.; Ajibade A. O.; Adah A. D.; Oloko A. B.; Bamigboye O. F.Farming of hybrids of two African Clariid catfish species, “Heteroclarias” is becoming a focus of attention in many aquatic farms in Nigeria. Pseudomonas sp. is one of the major pathogens responsible for disease in aquaculture. A total of 150 Heteroclarias juveniles were orally inoculated with different concentrations of Pseudomonas aeruginosa ranging from 3 x 101 to 3 x104 CFU/100µl in 4 groups (A to D) replicated thrice, for 14 days with the aim of evaluating its pathogenicity. The control group (E) also with 10 fish in 3 replicates were given normal saline orally. Clinical signs, mortality, histopathology and haematology were observed following experimental infection. Haematological parameters observed were statistically analysed using SPSS 20. Clinical signs observed in group A to C were discolouration, sloughing and patches of haemorrhage on their skin, Frequency of mortality recorded in group A to E were 20%, 10%, 10%, 6.6% and 0% respectively. Histopathological changes observed were vacuolar degeneration of renal tubular epithelium, diffuse hepatocellular vacuolar degeneration, and hyperplasia, fusion of secondary lamellae, with haemorrhage in the gills. Significant difference in haemoglobin concentration parameter was observed, translating to reduction in oxygen carrying capacity of the infected fish. Heteroclarias were susceptible to Pseudomonas aeruginosa, with resultant significant mortality.Item Clinicopathological features of arborescent organ necrosis syndrome in Clarias gariepinus (Burchell 1822)(Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria, 2013) Oladosu G. A.; Tijani M. O.; Akpavie S. O.; Adedeji O. B.Some teleosts such as the African catfish (Clarias gariepinus) posess accessory respiratory organs (arborescent organ) which enable them to breathe atmospheric air in hypoxic conditions. Arborescent organ necrosis syndrome is a relatively new disease condition of farmed African catfish in Nigeria. There is only one report of this syndrome in the African catfish, although several anecdotes of its occurrence exists. Six freshly dead catfishes were collected from two farms during a disease outbreak where severe high mortalities were recorded. At necropsy there was severe necrosis of the arborescent organ. Other gross lesions observed included severe gill hyperaemia and severe depigmentation of the skin. Histopathology revealed severe necrosis and non- suppurative inflammation of the arborescent organ and gills, hepatocellular vacuolar degeneration and severe necrosis of the haematopoietic and non haematopoietic elements of the trunk kidney. Three organisms namely Aeromonas hydrophila, Pseudomonas putida and Salmonella subsp IIIB were isolated from the necrotic arborescent organs and the dorsal and trunk kidneys. All the isolates were susceptible to ciprofloxacin. No mortalities were recorded on affected farms a day following the administration of ciprofloxacin to affected catfishes. Although this is not the first report of this syndrome in the African catfish, it is notable that the organisms isolated in this case differ from what was isolated (Enterobacter cloacae and Aeromonas sobria) in the previously reported case.