recovery rates of mycobacterium tuberculosis using five decontamination methods

Abstract

tuberculosis (TB) caused by mycobacterium tuberculosis remains one of the leading infectious diseases in resource limited countries of the world, including Nigeria. For optimal care of patients with pulmonary TB, effective decontamination methods are required for isolation and identification of M. tuberculosis from other fast growing organisms found in sputum samples of infected patients. five methods of sputum decontamination including the petroff,oxalic,bleach,simplified concentration and kodoh methods were assessed prior to mycobacterial culture. in all, thirty human sputum samples were processed and results analysed after eight weeks of incubation. overall, there was a significant difference in the growth yield using the different methods (friedman test statistic,Q =36.3; p<0.05). again,a significant difference (friedman test statistic,Q = 48.0; P <0.05) was observed between the valuable and non-valuable yield of mycobacteria. furthermore, the simplified concentration method had the best performance in terms of pure culture growth/minimal media contamination coupled with a cost benefit ratio of 0.10; the bleach method being the least. given these findings,coupled with laboratory challenges in developing countries as well as ease of use on the field/cost effectiveness: we propose the simplified concentration as an optimal decontamination method for use in resources limited settings where TB remains an endemic problem. organismes a croissance rapide qui se trouvent dans les echantillons de crachat des patients infectes. cinq methodes de decontamination du crachat y compris les methods de petroff, oxalique, de blanchiment, de concentration simplifiee et de kudoh ont ete evaluees avant la culture des mycobacteries, en tout, trente echantillons de crachat humains ont ete traites et les resultats analyses apres huit semaines d'incubation. de facon generale, il y avait une difference considerable dans les rendements de croissance en utilisant les differentes methods(test statistique de friedman,q =36.3; p<0.05) aussi, on a remarque une difference importante(test statistique de friedman, Q = 48.0; p<0.05) entre le rendement important et peu important de mycobacterie. de plus, la methode de concentration simplifiee a eu une meilleure performance en termes de croissance de culture propre/ contamination minimale de madia ajoutee a un ratio de profit de laboraroire dans les pays en developpement de menne que la facilite de l'usage sur terrain/efficacite du cout; nous proposons la concentration simplifiee comme methode de decontamination optimale l'usage des pays a ressources limitees ou la TB demeure un probleme endemique.