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    Phenotypic Characterization And Spoligotype Profiles Of Mycobacterium Bovisisolated From Unpasteurized Cows’ Milk In Ibadan, Nigeria.
    (2007) CADMUS, S.I.B.; ADESOKAN, H.K.
    A pilot study was carried out to screen for the presence of Mycobactcria in unpasteurized milk from cows mcant for sale at the cattle market in the mclropolitan city of Ibadan. South-western Nigeria. Fifty three milk samplcs were cultured using 7H11 -Middlebrook media while nitrate and niaein biochemical tests were carried out initially to classify the isolated Mycobacteria specics and the results were later' confirmed by spoligotyping. In all, Mycobacterium bovis were isolated from 6 (11.3%) milk samples. The detected spoligotypes obtained were NI, N6, N9, NI6. and N28 (two isolates). After comparison with the "Mycobacterium bovis Spoligotype Database” (www.mbovis.org), spoligotypes NI. N6, and N16 were found to have been already documented (N1=SB0944; N6=SBI027: NI6=SB0951). while N9 and N28 were relative!)' new strains and undocumented. The public health implication of these findings is that humans are at great risk of exposure to infection with M. bovis through the consumption of unpasteurized milk from cattle through the food chain.
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    Serological survey of brucellosis in livestock animals and workers in Ibadan, Nigeria
    (2006) Cadmus S. I. B.; Ijagbone I. F.; Oputa H. E.; Adesokan H. K.; Stack J. A.
    A serological survey of brucellosis in livestock animals and workers was conducted in Ibadan, Southwestern Nigeria between May and August 2004. A total of 1,210 cattle, 54 sheep, 496 goats, 200 pigs and 21 humans (i.e. butchers and herdsmen) were screened using the Rose Bengal test (RBT).From the results, prevalence in trade cattle was 5.82% while 0.86% was recorded in goats. None of the sheep and pigs was positive to the test. Out of the 11 samples taken from butchers, seven were positive and none of the ten herdsmen were sero-positive. This gives an infection rate of 63.3% in the butchers; and a combined infection rate of 31.82% in humans. This shows that brucellosis is still a major zoonosis in Nigeria; other public health implications are discussed.