Veterinary Medicine

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A case report of Dystocia due to shistosomus reflexus (conjoined twins) in a West African Dwarf Doe in Ibadan, Nigeria
(Faculty of Veterinary Medicine, University of Ibadan., 2017) Olaogun, S. C.; Abiola J. O.; Jeremiah, O. T.
A three years old light brown West African dwarf doe, weighing about 32 kg was presented with history of dystocia of about 36 hours was presented to the University of Ibadan Veterinary Teaching Hospital, Ibadan. The animal was restless. Close observation showed a swollen vulva with protruding foetal head. There was abnormal slimy blood tinted discharges with foul odour in her vulva. Rectal temperature was 40 degree Celcius with relatively high respiratory rate. Dystocia was diagnosed. Using systematic and calculated tration and retropulsion, Schistosomus reflexus (co-joined twins) were subsequently delivered after about 20 minutes of intervention. Post-manipulation care was given by intramuscular injection of procaine penicillin and streptomycin combination at a dose of 1ml per 10kg body weight for 4 days consecutively; multivitamins injection was also given at 1ml per 10kg body weight for four days. Complete recovery of the doe was achieved.
A case report of Dystocia in a primiparous West African dwarf ewe due to relative fetal oversize associated with poor mating management practices in Ibadan, Nigeria
(Faculty of Veterinary Medicine, University of Ibadan, Nigeria, 2019) Olaogun, S. C.; Olaifa A. K.; Odunkoya, D.
A one and a half (1½) year old Primiparous West African dwarf ewe, weighing 12 kg was presented with history of anorexia and straining of about 24 hours duration to the University of Ibadan Veterinary Teaching Hospital, Ibadan. Close observation of the animal showed a narrow birth canal with bilateral carpal flexion of the foetus which also appeared too big for the dam. Rectal temperature was 36.9°C, Heart rate was 68beats per minute and respiratory rate was 36 breathes per minute. The dam was reported to be mated with a ram of same breed weighing 40kg. Dystocia was diagnosed and caesarean section was recommended to relieve the dystocia. The right lateral side of the abdomen close to the Paralumbar fossa was carefully and aesthetically shaved and prepared. Induction of anaesthesia was done using 2 ml of Lignocaine given epidurally as an inverted L-block and 1 ml of Duracaine was given posteriorly. Complete desensitization of the region was achieved within 5minutes of induction. Drapes were used to prevent contamination as much as possible. A small straight 10cm long incision was made on the lateral side with no obvious bleeding observed. The uterus was located and incised with the apparently big dead foetus weighing 2.2kg taken out and chromic catgut size 1 was used to suture the uterus. The skin was about to be sutured with nylon suture material when the dam passed on. This report highlighted the detrimental effect of poor reproductive management practices and the delay in seeking veterinary care leading to maternal and fetal death with its grave economic consequences.
A case report of Management of impaction colic in Donkey in Ibadan, Nigeria
(Faculty of Veterinary Medicine, University of Ibadan, Nigeria, 2020) Adedokun, R. A. M.; Olaifa, A. K.; Alaba, B. K.; Olaogun, S. C.
A 13 year old male donkey (jack), weighing 171 kg was presented with history' of frequent prolonged recumbency and eliciting sand bath action. The donkey was dehydrated, anorexic, inactive, refused to drink and no defecation. Rolling, bloating, uneasiness, distress were also observed, grunting sound was also heard whenever the animal attempted to rise from recumbency. Rectal temperature was 36.6°C, pulse and respiratory rates were 60 and 30 per minute, respectively. Mucous membrane initially appeared normal but later became congested with generalised bruises on the body of the donkey especially at the bony prominences. Colic was clinically diagnosed and treatment immediately instituted. Animal was drenched with 1 litre of liquid paraffin; 2 litres of lactated Ringer’s solution was given intravenously, I/V; lntramuscular(l/M) injections of 8 mis (2.25mg/Kg) of vitamin B complex and 3.8 mis of50mg/ml (1.1 mg/Kg) Flunixin Meglumine were also administered. The patient was treated for five consecutive days with significant improvement on the third day. The rolling, bloating, distress and uneasiness were relieved after day four of treatment and the donkey was discharged on the fifth day. This report highlighted the causes, type and nature of this particular case, management procedures and preventives strategy of colic in donkey.
A case report of Management of impaction colic in Donkey in Ibadan, Nigeria
(Faculty of Veterinary Medicine, University of Ibadan, Nigeria, 2020) Olaogun, S. C.; Adedokun R. A. M.
Ram fighting is one of the unimaginable sports practiced all around the World. It is often associated with all sorts of injuries which may predispose animals to tetanus. Tetanus is an acute, severe and potentially fatal toxin-mediated disease caused by Clostridium tetani infection. This organism thrives under anaerobic conditions, such as unclean environment, contaminated wounds. A case of an adult West African Dwarf Ram, weighing 45kg presented with generalized stiffness, mild opistotonus, difficulty in swallowing and signs of generalized pain was presented. On physical examination, rapid breathing and mild bloat were observed, but every other clinical parameter was within the normal reference values. Tentative diagnosis of tetanus associated with injuries sustained during animal figthing. The patient was treated with human tetanus immunoglobulin followed by intravenous metronidazole, procaine penicillin streptomycin and diazepam injection administered intramuscularly. High dose of multivitamins was administered I/M. The symptoms were relieved after three hours of intensive treatment each day for two consecutive days. The client failed to present the patient to the clinic for follow-up on the third day citing cost and distance to the clinic as excuses. To the authors’ knowledge a tetanus infection associated with injury from Ram fight has not been previously reported in Ibadan, Nigeria.
A case report of management of pasture bloat in a post-parturient West African Dwarf Ewe in Ibadan, Nigeria
(Faculty of Veterinary Medicine, University of Ibadan, Nigeria, 2020) Olaogun, S. C.; Adedokun, R. A. M.
Black anterior and white posterior body coat was presented at the Veterinary Teaching Hospital, University of Ibadan, with complaints of abdominal distension, sudden laboured breathing and anorexia. The animal was said to have lambed 7 days earlier and was fed with cassava peels and grasses. On clinical examination, the eye ball appeared bulged and starry; the stomach was hard on palpation with frothy buccal exudate and persistent grinding of teeth. The heart rate was 68/minute, respiratory rate was 15/minute and rectal temperature was 39.8oC. Bloat was clinically diagnosed. With the patient properly restrained, an improvised stomach tube was rinsed in vegetable oil and slowly inserted through the oesophagus into the rumen and the gas eructated. Liquid paraffin (15 mls) was subsequently introduced into the rumen via the tube. On removing the stomach tube, a long strand of fresh undigested pasture came out with the tube from the gastrointestinal tract. The hard stomach was thereafter relieved within 5 minutes of the procedure. Long acting Oxytetracycline hydrochloride (2 mls) and 1ml Diclofenac were administered (intramuscularly) simultaneously at different injection sites. Plenty of water was advised to be given orally after 30minutes. Complication resolved and complete recovery of the ewe was achieved after 5 consecutive days of treatment.
A cryptic mitochondrial DNA link between North European and West African dogs
(Elsevier, 2017) Adeola, A. C.; Ommeh, S. C.; Song, J. J.; Olaogun, S. C.; Sanke, O. J.; Yin, T. T.; Wang, G. D.; Wu, S. F.; Zhou, Z. Y.; Lichoti, J. K.; Agwanda, B. R.
Domestic dogs have an ancient origin and a long history in Africa. Nevertheless, the timing and sources of their introduction into Africa remain enigmatic. Herein, we analyse variation in mitochondrial DNA (mtDNA) D-loop sequences from 345 Nigerian and 37 Kenyan village dogs plus 1530 published sequences of dogs from other parts of Africa, Europe and West Asia. All Kenyan dogs can be assigned to one of three haplogroups (matrilines; clades): A, B, and C, while Nigerian dogs can be assigned to one of four haplogroups A, B, C, and D. None of the African dogs exhibits a matrilineal contribution from the African wolf (Canis lupus lupaster). The genetic signal of a recent demographic expansion is detected in Nigerian dogs from West Africa. The analyses of mitochondrial genomes reveal a maternal genetic link between modern West African and North European dogs indicated by sub-haplogroup D1 (but not the entire haplogroup D) coalescing around 12,000 years ago. Incorporating molecular anthropological evidence, we propose that sub-haplogroup D1 in West African dogs could be traced back to the late-glacial dispersals, potentially associated with human hunter-gatherer migration from southwestern Europe.
A Retrospective Study of Ruminant Cases Presented Between 1996 and 2005 at the Veterinary Teaching Hospital, University Of Ibadan, Ibadan, Nigeria
(Integrity Research Journals, 2016) Abiola, O. J.; Olaogun, S. C.; Emedoh, O. M.; Jeremiah, O. T.
Retrospective study of ruminant cases (cattle, sheep and goats) presented to the Veterinary Teaching Hospital, University of Ibadan, Ibadan between a period of ten years (January1996 to December 2005) was conducted to determine the most prevalent diseases using clinical case file records. Within the period of study, a total of 601 cases were handled out of which 394 (65.56%) occurred in goats, 171(28.45 %) sheep and 36(5.99%) cattle. The most prevalent infections in the three species were ectoparasitism (31.11%), endoparasitism (16.97%) and reproductive cases (13.31%). Among caprine species, endoparasitism was 27.16%, while ectoparasitism and reproductive conditions had 20.96% and 13.96% respectively. As for ovine species endoparasitism was 38.01% while ectoparasitism 9.94% and reproductive conditions had 9.94. But among bovine species, endoparasitism was 41.67%, reproductive conditions had 22.22% and bacterial diseases had 16.67%. The high prevalence of these conditions may be due to poor management practices, improper health/veterinary care, stress on pregnant animals, and neglect of prophylactic measures for disease prevention. There is therefore need for awareness and education of livestock farmers/owners on effective prevention and control measures through livestock extension services and proper management system that restrict animals from roaming freely thereby preventing injuries caused by trauma.
Analysis of the genetic variation in mitochondrial DNA, Y-chromosome sequences, and MC1R sheds light on the ancestry of Nigerian indigenous pigs
(Springer Nature, 2017) Adeola, A. C.; Oluwole, O. O.; Oladele, B. M.; Olorungbounmi, T. O.; Boladuro, B.; Olaogun, S. C.; Nneji, L. M.; Sanke, O. J.; Dawuda, P. M.; Omitogun, O. G.; Frantz, L.
Background: The history of pig populations in Africa remains controversial due to insufficient evidence from archaeological and genetic data. Previously, a Western ancestry for West African pigs was reported based on loci that are involved in the determination of coat color. We investigated the genetic diversity of Nigerian indigenous pigs (NIP) by simultaneously analyzing variation in mitochondrial DNA (mtDNA), Y-chromosome sequence and the melanocortin receptor 1 (MC1R) gene. Results: Median-joining network analysis of mtDNA D-loop sequences from 201 NIP and previously characterized loci clustered NIP with populations from the West (Europe/North Africa) and East/Southeast Asia. Analysis of partial sequences of the Y-chromosome in 57 Nigerian boars clustered NIP into lineage HY1. Finally, analysis of MC1R in 90 NIP resulted in seven haplotypes, among which the European wild boar haplotype was carried by one individual and the European dominant black by most of the other individuals (93%). The five remaining unique haplotypes differed by a single synonymous substitution from European wild type, European dominant black and Asian dominant black haplotypes. Conclusions: Our results demonstrate a European and East/Southeast Asian ancestry for NIP. Analyses of MC1R provide further evidence. Additional genetic analyses and archaeological studies may provide further insights into the history of African pig breeds. Our findings provide a valuable resource for future studies on whole-genome analyses of African pigs.
Antibiograms of Bacteria Isolated from Diseased Lungs of Cattle Slaughtered at Abattoir, Ibadan, Nigeria
(Faculty of Veterinary Medicine, University of Nigeria, Nsukka, 2022) Uchechukwu, C. O.; Amosun, E. A.; Jeremiah, O. T.
This study was undertaken to assess the antimicrobial susceptibility profile of bacterial pathogens isolated from diseased lungs of cattle. Forty-two bacterial isolates obtained from diseased lung tissue samples of cattle slaughtered at Bodija abattoir were tested for antimicrobial susceptibility patterns using the Kirby-Bauer disc diffusion method. The bacteria isolated were Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas species, Proteus mirabilis, Pasteurella lymphangitidis, Pasteurella multocida, Pasteurella ureae, Pasteurella pneumotropica, Klebsiella pneumoniae, Escherichia coli, Morganella morganii and Mannheimia haemolytica. Selection of antibacterials was on the basis of the Gram reaction of the isolates and each was tested for 10 different antibacterials. A high proportion of the Gram-negative isolates showed sensitivity to ciprofloxacin (75.0 %) and ofloxacin (66.7 %) while a moderate proportion (54.2 %) was sensitive to perfloxacin. A low proportion of the Gram-negative isolates showed sensitivity to amoxicillin/clavulanate (20.0 %), tetracycline (20.8%), amoxicillin (20.8%), trimethoprim (12.5%), sulfamethoxazole (12.5%) and gentamycin (4.2%) while resistance was demonstrated against nitrofurantoin and ceftriaxone. All the isolates of Streptococcus pneumonia were sensitive to cefuroxime, ceftriaxone, ciprofloxacin, streptomycin, trimethoprim/sulfamethoxazole, perfloxacin and chloramphenicol, with very low proportion being sensitive to ampicillin and amoxicillin. Most of the isolates of Staphylococcus aureus were resistant to all the drugs tested. The results of the study showed that the isolates were resistant mostly to the common and relatively cheaper drugs; an important economic impact to the chemotherapeutic management of respiratory disease in cattle.
Antibiotic profiling of bacterial isolates obtained from turkey and chicken in selected farms in Ibadan, Nigeria
(Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria, 2019) Amosun, E. A.; Adepoju, B. C.; Ogunbadewa, A. J.; Abatan, M. O.
In recent times, the prevalence of antimicrobial drug resistance has increased tremendously due to a number of factors including use of human drugs for the treatment of animal diseases, leading to the transfer of antibiotic resistance in terms of antibiotic residues in poultry meat to pathogenic bacteria. This study determined the antibiotic profiles of bacterial isolates in poultry cloacal swabs from selected farms in Ibadan. Fifty and twenty cloacal swabs were collected aseptically from turkey and chicken at Apete and University of Ibadan research farm respectively. The samples were immediately transported to the laboratory for microbiological analysis. Thus, the cloacal swabs were screened using MacConkey agar, blood agar and xylose lysine deoxycholate agar. Isolates were identified using standard microbiological techniques and tested to ten different antibiotic discs according to Kirby-Bauer procedure. Sixty-one and thirteen different isolates were detected from turkey and chicken cloacal swabs respectively. Of the turkey isolates, Pseudomonas had the highest occurrence of 25% while Escherichia coli (46%) had the highest occurrence of the chicken isolates. The Gram-negative isolates showed high resistance to augmentin (69%), streptomycin (69%), sulphamethoxazole (78%) and chloramphenicol (82%). Staphylococcus species which was the only Grampositive isolate in this study was greatly resistant to gentamicin (83%). Both the turkey and chicken isolates had different antibiotic resistance rates and patterns with a huge percentage (86%) of them being multi-drug resistant. This work observed a higher resistance to many of the commonly used antibiotics in the poultry industry thereby, posing a public health risk since most of these drugs are used for treatment of human infections
Antibiotic Resistance Profiling and Microbiota of the Upper Respiratory Tract of Apparently healthy Dogs in Ibadan, South West Nigeria
(Drug Research & Production Unit, Faculty of Pharmacy, Obafemi Awolowo University,, 2017) Daodu, O. B.; Amosun, E. A.; Oluwayelu, O. D.
Background: Rearing of dogs and other pets has become increasingly popular in modem society. Bacterial flora resides within thè nasal and orai cavities of dogs and when chanced, can be pathogenic. Certain similarities between humans and dogs portends dangerous behavioral habits that could lead to zoonotic disease transmission. This study was aimed at isolation, identification and antibiotic profiling of bacteria from nasal swabs of apparently healthy dogs. The zoonotic risk was also considered. Methodology: A total of 173 nasal swabs were collected from 173 apparently healthy dogs. Structured questionnaires were administered to investigate human behavioral habits. Results: Two hundred and twenty two (222) bacterial isolates were obtained from thè culture with ten (10) potentially pathogenic bacteria in thè order of Escherichia coli (18.5%), Proteus species (17.1%), Staphylococcus aureus (14.0%), Klebsiella species (9.0%), Acinetobacter species (9.0%), coagulase negative Staphylococcus species (7.7%), Pseudomonas species (6.8%), Actinobacter species (6.8%), Citrobacter species (5.9%) and Streptococcus species (5.4%). Overall, thè Gram negative isolates showed resistance to ciprofloxacin (9.3%), sparfloxacin (16.0%),perfloxacin (17.3%), ofloxacin (21.6%), chloramphenicol (34..6%), gentamycin (36.4%), streptomycin (37.%), septrin (49.4%), amoxillin (59.3%), augmentin (62.3%) while thè Gram positive bacteria showed resistance to ciprofloxacin (3.3%), perfloxacin (6.7%), erythromycin (13.3%), streptomycin (21.7%), rocephin (28.3%), septrin (28.3%), gentamycin (36.7%), zinnacef (68.3%), ampiclox (81.7%) and amoxillin (85.0%). Multi-dmg resistance (MDR) to three or more antimicrobials was observed in some of thè isolates. Seventy - seven resistance pattems were observed, 16 in Gram positive and 61 in Gram negative bacteria. Conclusion: This study revealed MDR to two or more antimicrobials in all thè isolates. These can pose antibiotic resistance challenges in situation of primary or secondary canine respiratory infections. Also, this study revealed that 82% of thè dog owners/ lovers had less than 50cm face-to-face contact with these dogs while playing with them, thus increasing their chances of acquiring MDR bacteria from apparently healthy dog
Antibiotic Resistance Profiling of Escherichia coli O157:H7 from Guinea fowl and Chicken in Ibadan North Local Government area, Nigeria
(Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria, 2017) Amosun, E. A.; Ayantoke, I. A.; Agba, J. U.; Ojo, O. E.
Escherichia coli Ol 57 is a major zoonotic disease of thè poultry industry. 315 cloacae samples (177 chickens and 138 guinea fowls) were screened for enterohaemorrhagic E. coli (EHEC 0157). The samples were pre-enriched with tryptic soy broth, incubated over- night at 37°C for 18 hours, sub-cultured on MacConkey agar and incubated at 37°C for 24 hours. Rose pink colonies on MacConkey agar (putative E. coli) were selected for bio- chemical test. The susceptibility of identified E. coli isolates to antimicrobial agents was determined by thè standard Kirby-Bauer disk diffusion method on Mueller-Hinton agar. Confirmed E. coli were sub-cultured on Sorbitol MacConkey agar piate (SMAC) and incubated at 37°C for 24hours. Colourless or pale colonies (non-sorbitolfermenters) were further tested. Isolates that produced colourless colonies and agglutinatimi with E. coli 0157 latex test were tested with E. coli HI antiserum by a slide agglutination test as well as other identified E. coli isolates irrespective of their sorbitol fermentatimi reactions were identified serologically using a latex agglutination test kit. Of thè 315 samples, 9.5% were positive for EHEC Ol 57 including 7.3% and 12.3% of thè chickens and thè guinea fowls, respectively. The differences in thè two species were not statistica!ly (P<0.05) significant The resistance of thè EHEC 0157 to antim icrobials were; amoxicillin/clavulanic acid 66.67%, nitrofurantoin 60.00%, gentamicin 56.67%, trimethoprim/sulphamethoxazole 50.00%, tet- racycline 50.00%, pefloxacin43.33%, amoxicillin 33.33 %, ciprofloxacin 33.33 %,ofloxacin 30.00%, and ceftriaxone 20.00%. E. coli OJ57:H7 isolated from guinea fowls were all susceptible to amoxicillin and ciprofloxacin while EHEC 0157:H7 from chickens showed 100% resistance to tetracycline and pefloxacin. The presence of virulent multidrug resistali E. coli 0157 strains in thè faeces of guinea fowls and chickens showed thè dangers that handlers and consumers are exposed and thè implications of disseminatimi of thè pathogens to thè environment through faecal shedding.
Antibiotic sensitivity of some bacteria isolates isolated from scouring calves from some farms in Oyo and Ogun States, Nigeria
(Faculty of Veterinary Medicine, University of Ibadan, Nigeria, 2018) Olaogun, S. C.; Jeremiah, O. T.; Jubril, A. J.
Diarrhoea in young pre-weaned calves is one of the most important causes of calf morbidity and mortality. Information on this multi-aetiological condition is scanty in Nigeria. Therefore, we studied the prevalent bacteria and antibacteria susceptibility to various bacterial isolates from faecal samples of suspected cases of calf scours in Oyo and Ogun States of South-western Nigeria. One hundred and twenty calf faecal samples (120) from various breeds of cattle showing clinical manifestation of scours were screened for bacterial agents in two farms in Oyo and five farms in Ogun States. Isolation and identification were done using standard procedures, while antibiotic sensitivity was done using Kirby Bauer's disc diffusion method. Escherichia coli, Salmonella spp. and Campylobacter spp. were identified. Out of all the calves, 19 calves (15.8%) were positive for bacteria agents, E. coli was isolated from 12 calves (10%), Salmonella spp. was isolated in 10 calves (8.3%) and Campylobacter spp. was isolated in 1 calf (0.8%). Antibiotic sensitivity test revealed that the most sensitive antibiotics were Meropenem (91.3%) and Amikacin (82.6%) while the least sensitive was Ampicillin (21.7%). Therefore Meropenem is the most sensitive antibiotic and recorded the highest percentage sensitivity in all the three bacteria pathogens isolated, while Ampicillin appeared to be the least sensitive antibiotic to virtually all the pathogens isolated. Meropenem is therefore recommended as the drug of choice for the management of bacteria calf scours. The isolation of highly pathogenic E.coli 0157 from calf scour cases reaffirmed cattle as a reservoirs host which may enter the food chain and poses a faeco-oral potential public health hazards.
Antimicrobial resistance among commensal Escherichia coli from cattle faeces and beef in Ibadan, Nigeria
(Academic Journals, 2012) Amosun, E. A.; Ojo, O. E.; Alao, I. K.; Ajuwape, A. T. P.
Commensal bacteria contribute to the distribution and persistence of antimicrobial resistance in the environment. This study monitored antimicrobial resistance in commensal Escherichia coli from the faeces of on-farm and slaughter cattle and beef. A total of 342 (89.5%) E. coli isolates were obtained from 382 samples. Isolation rate of E. coli was 90.0% in on-farm cattle, 87.1% in slaughter cattle and 92.2% in beef. Overall, the isolates showed resistance to amoxicillin (97.9%), ampicillin (97.9%), cefuroxime (25.1%), chloramphenicol (69.3%), ciprofloxacin (11.7%), cotrimazole (45.9%), erythromycin (59.4%), gentamycin (36.5%), nalidixic acid (27.2%), nitrofuratoin (54.9%), norfloxacin (21.1%), ofloxacin (14.0%), streptomycin (78.9%) and tetracycline (33.9%). There were no significant differences in antimicrobial resistance of E. coli from the different sample types. Only four (1.2%) of the 342 isolates were susceptible to all antimicrobial agents, while 338 (98.8%) were resistant to at least one of the tested antimicrobial agents. Multi-drug resistance to three or more antimicrobials was observed in 321 (93.9%) of all the isolates. Forty-one resistance groups were observed in on-farm cattle, 30 in slaughter cattle and 34 in beef. All the 30 resistance groups found in slaughter cattle were also present in on-farm cattle and beef. ‘AmoAmpChlEryNitStr’ and ‘AmoAmpChlStr’ were the predominant resistant patterns. This study confirmed on-farm and slaughter cattle as important sources of antimicrobial resistant E. coli transmissible to humans through beef.
Antimicrobial Resistance in Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa Isolated from the Milk of Dairy Cows in Three Nigerian Cities
(Nigerian Veterinary Medical Association, 2012) Amosun, E. A.; Olatoye, O. I.; Adetosoye, A. I.
This study determined the safety of milk from dairy herds obtained by hand milking method from two major cattle producing States of Nigeria by investigating the presence of Escherichia coli 0157:H7 and their antibiotic susceptibility pattern. Forty seven milk samples from Kwara and 63 from Kaduna States were obtained from selected indigenous breeds of dairy cow. Bacteriological analysis by culturing on MacConkey agar (MAC) and subcultured on Sorbitol MacConkey agar (SMAC) were done. Escherichia coli 0157.H7 were confirmed serologically using latex agglutination kits (OxoidR UK). The isolates were tested for susceptibility to five commonly used antimicrobial agents and plasmid transfer was also carried out using E. coli K12 356 recipient. Out of the 61 non-Sorbitol fermenting (NSF) E. coli isolated from the samples 33(30.0%) were confirmed as E .coli 0157.H7 serotype. Antibiotic Susceptibility profile showed that all the isolates were resistant to one or multiple antibiotics, resulting in six different resistance patterns. Sulphadimidine resistance was the highest with all the isolates (100%) exhibited resistance to this drug while streptomycin had the highest sensitivity. Out of the seventeen E.coli 0157.H7 isolates tested for plasmid transfer, eleven (64.7%) transferred their resistance to the sensitive E. coli K12 356 enblock, while the remaining six showed segregation. The preponderance of E. coli O157 in this study indicated that greater proportion of milk being produced for human consumption in Nigeria were not wholesome and could posed threat of transmission of zoonotic pathogens. The high proportion of multidrug resistance exhibited by the isolates and the plasmid transfer is of public health significance as they could confer resistance on both pathogenic and non-pathogenic bacterial population in the consumers. More so, the milk which were obtained traditional unhygienic hand milking are either consumed raw or improperly pasteurized. Hygienic milking, pasteurization and judicious use of antibiotics after properly diagnosis and sensitivity test against newer antibiotics are recommended.
Antimicrobial Resistance in Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa Isolated from the Milk of Dairy Cows in Three Nigerian Cities
(Nigerian Veterinary Medical Association, 2012) Amosun, E. A.; Olatoye, O. I.; Adetosoye, A. I
Bovine mastitis is usually associated with bacterial pathogens isolated from the milk or mammary glands of dairy cows. A total of 205 isolates comprising of 110 (53.66%) Escherichia coli, 67 (32.68%) Klebsiella pneumoniae, and 28 (13.66%) Pseudomonas aeruginosa obtained from cases of bovine mastitis from southwest and Northern Nigeria during a period of one year were tested for susceptibility to ampicillin, tetracycline, neomycin, streptomycin, sulphadimidine and nalidixic acid using minimum inhibitory concentration (MIC) method. The results demonstrated wide variation of in the susceptibility patterns for the various organisms from different regions of Nigeria. The three organisms displayed highest resistance to sulphadimidine 200 (97.5%) followed by ampicillin 153 (74.63%), tetracycline 103 (50.24%), neomycin 90 (43.90%), streptomycin 68 (33.17%) and nalidixic acid 29 (14.15%) respectively. The resistance patterns of the strains revealed 27 distinct resistance groups. In conclusion these data confirmed that majority of the Gram negative organisms that are causative agents of mastitis in Nigeria were resistant to several antibiotics. This could be a result of indiscriminate use of drugs by dairy farmers for treatment of mastitis in their herds.
Antimicrobial resistance pattern of Streptococci and Staphylococci isolated from cases of bovine clinical mastitis in Nigeria
(Marsland Press, USA, 2012) Amosun, E. A.
Streptococci and staphylococci are frequently isolated from bovine mastitis in dairy cows. Limited information is available on the antimicrobial susceptibility of these organisms in Nigeria. A total of 130 Streptococci and 177 Staphylococci isolated from cases of bovine mastitis from three states in Nigeria namely; Oyo, Kwara and Kaduna states for a period of one year were used in this study. Overall, 55.38% of the strains tested were Streptococcus uberis, 24.62% were Streptococcus agalactiae, 12.31% were Streptococcus dysgalactiae, 3.85% were Streptococcus zooepidemicus, 2.31% were Streptococcus bovis and 1.54% were for Streptococcus equines, 25 coagulase negative staphylococcus and 152 Staphylococci aureus. The antimicrobial susceptibility for these organisms was determined for the following antimicrobial agents: Ampicillin, Tetracycline, Sulphadimidine, Nalidixic acid, Neomycin and Streptomycin. Results demonstrated substantial differences in their resistance patterns for the various organisms. The resistance patterns revealed 10 distinct resistance groups. All the streptococci isolate showed resistance to Ampicillin and tetracycline while 98.46%, 86.15%, 48.46%, 24.62%, and of the Streptoccoci species were resistance to sulphadimidine, Neomycin, Streptomycin and Nalidixic acid respectively while Staphyloccocus aureus had 100% for tetracyclin and sulphadimidine respectively while 97.37%, 88.16%, 69.08% and 67.11% showed resistance to ampicillin, neomycin, nalidixic acid and streptomycin respectively. However, Staphylococcus coagulase negative showed 100% resistance to tetracycline and sulphadimidine while 72%, 68%, 68%, and 32% showed resistance to nalidixic acid, neomycin, ampicillin and streptomycin respectively. The result of this study revealed that dairy farmers misused these antibiotics by treating cows several times per case. This study point to the fact that dairy farmers should take caution in the use of antibiotics for the dairy cows on the farms
Antimicrobial Resistance Profiling of Escherichia coli Isolated from Chickens in Northern Province of Rwanda.
(Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria, 2022) Cyuzuzo, E.; Amosun, E. A.; Byukusenge, M.; Musanayire, V.
Antimicrobial resistance (AMR) is a global public health concern due to inappropriate antimicrobial-use in humans and animals including poultry. Escherichia coli have been proposed as one of the pathogens to be used for AMR surveillance. The goal of current study was to determine the antimicrobial resistance of E. coli in chickens in Northern Province of Rwanda. A cross- sectional study was conducted between June and August 2021; the chickens were randomly selected in each of the twenty farms that were included in the study. The samples were collected from cloaca and from farm environment. E. coli was isolated and identified and antimicrobial susceptibility testing was done using disc diffusion method. The results were defined as resistance(R), susceptible(S) and intermediate (I). After Data processing, they were entered in Microsoft Excel for analysis. The data were presented as frequencies and percentages. In total, 384 samples were collected (139 in Gakenke, 114 in Rulindo and 131 in Musanze Districts). E. coli was isolated from 162(42.18%) among which 40 (24.7%) were from Musanze District, 57(35.2%) from Gakenke District and 65 (40.1%) were from Rulindo District. The highest resistance was observed for tetracycline (69.8%) followed by cotrimoxazole (39.5%). The highest susceptibility was observed for gentamycin (100%) followed by ciprofloxacin (96.9%) and amoxicillin (66%). This study indicated the presence of E. coli in chickens of Northern Province of Rwanda and a large number of antibiotic resistant E. coli were isolated. Therefore, the government should set policies to control the use of antibiotics on farms.
Antimicrobial Susceptibility in Escherichia coli isolates from Guinea Fowls (Coturnix coturnix japonica) and Quails (Numida meleagris) in Ibadan, Oyo-State, Nigeria
(Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria, 2021) Amosun, E. A.; Martin, A. E.
Poultry are reared for meat, eggs and feathers. Poultry production is classified into four sectors which are chickens, ducks, guinea fowl, turkey and geese based on the marketing of poultry products and the level of biosecurity. Intensive poultry farming falls under chickens, ducks, guinea fowl and turkey characterized by moderate to high levels of biosecurity, while geese pertain to the ‘backyard’, ‘village’ or ‘family’ poultry system, with few or no biosecurity measures. Avian colibacillosis is an infectious disease of birds caused by Escherichia coli which is considered as one of the principal causes of morbidity and mortality, associated with heavy economic losses to the poultry industry by its association with various disease conditions, either as primary pathogen or as a secondary pathogen. This study determined the prevalence and antibiotic susceptibility of Escherichia coli from guinea-fowl (Numida meleagris) and quail (Coturnix coturnix japonica) in Ibadan. A total of 180 cloacal sample was collected and bacteriologically analysed for the presence of Escherichia coli. For bacteriological analysis samples were pre-enriched in peptone broth and incubated overnight at 370C, followed by a selective isolation on MacConkey agar supplemented with ampicillin (lOOmg/L). Ampicillin-rcsistant isolates were subcultured on MacConkey agar supplemented with cefotaxime (lmg/L). Biochemical tests of all the cefotaxime resistant isolates was performed. Double disc diffusion test specific for Extended Spectrum Beta Lactamase producing E. coli (ESBL E.coli) was performed. Antibiotic sensitivity testing was done using Mueller-Hinton agar. Twenty -six isolates were analysed using disc diffusion method. Eighty - Four (84) isolates were lactose fermenters, 49 isolates were Ampicillin-rcsistant E. coli and 26 isolates were Cefotaxime-resistant E. coli and none was positive for ESBL-E. coli. Gentamycin has the highest sensitivity of 100% followed by ciprofloxacin of 96.2% of E. coli isolates while amoxicillin-clavulanic (96.2%). tetracycline (96.2%) and sulphamethaxazole (92.3%) has the highest rate of resistance. This present study investigated antimicrobial susceptibility of Escherichia coli isolated from guinea fowls and quail in Ibadan, Nigeria.
Antimicrobial Susceptibility Profiling and detection of Cefotaxime- Resistant Escherichia coli from Commercial Laying Hens, Indigenous Ducks and Chickens in Ibadan, Nigeria
(Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria, 2024) Amosun, E. A.; Kolapo, A. M.; Ojja, C. V.
Cefotaxime is a critically important antimicrobial agent for thè treatment of infections in humans and animals. The upsurge in thè incidence of cefotaxime-resistant Escherichia coti from animai sources is of global public health importance. Avian pathogenic Escherichia coti is a Gram negative zoonotic bacterial pathogen. Infections by Escherichia coti usually occur following consumption of foods and water contaminated with faeces. The development of antimicrobial resistance in Escherichia coti is a concem worldwide. This study evaluated thè prevalence, antimicrobial susceptibility and cefotaxime resistant Escherichia coti in commercial laying hens, indigenous ducks and chickens in Ibadan, Nigeria. Cefotaxime resistant Escherichia coti isolates from thè cloacae of these poultry sources were tested for antimicrobial agents. The overall isolation rate of cefotaxime resistant Escherichia coti was 6.5% (6/93), 3.2% (3/93) and 10.0% (20/200) from indigenous ducks, indigenous chickens and commercial laying hens respectively. Cefotaxime resistant Escherichia coti isolates were 89.7%, 86.2%, 65.5%, 55.2%, 37.9%, 27.6%, 20.7% and 20.7% resistant to sulphamethoxazole, tetracycline, ceftazidime, amoxicillin -clavulanic acid, chloramphenicol, ciprofloxacin, gentamicin and ceftriaxone respectively. Whereas, 75.9%, 68.9%, 62.1%, 51.7%, 41.4%, 27.6%,10.3% and 10.3% susceptible to ceftriaxone, ciprofloxacin, gentamicin, chloramphenicol, amoxicillin-clavulanic acid, ceftazidime, sulphamethoxazone and tetracycline respectively. Multidrug resistant (MDR) was observed in 89.7% (26/29) of thè isolates which exhibited 2 (in indigenous ducks), 3 (in indigenous chickens) and 13 (in commercial laying hens) different MDR pattems to 7 antimicrobial classes of drug. Higher isolation rate of cefotaxime resistant Escherichia coti and remarkable numbers of thè isolates from commercial laying hens showed multidrug resistant than that of indigenous ducks and chickens. Misused of drugs was predicted in commercial laying hens. This study showed that thè indigenous ducks and chickens harbour multidrug resistant Escherichia coti and may contribute to environmental contamination through faecal shedding.