FACULTY OF VETERINARY MEDICINE

Permanent URI for this communityhttps://repository.ui.edu.ng/handle/123456789/270

Browse

Author: search.filters.author.Odunola, O. A.

Search Results

Now showing 1 - 3 of 3
  • Thumbnail Image
    Item
    Anticlastogenic properties of methanolic extract of cnestis ferruginea leaves
    (Faculty of Pure and Applied Sciences, LAUTECH, Ogbomoso, Nigeria, 2006) Badmus, J. A.; Odunola, O. A.; Ola-Davies, O. E.; Adisa, R. A.
    The inhibitory activity of methanolic extract of Cnestis ferruginea was investigated in hone marrow cells of mice using micronucleus assay. The mice were fed with the extracts at two different concentrations (100 and 200mg/kg body weight) white control mice were fed with corn oil for six days ad libitum. On the seventh day the mice were administered a single dose of sodium arsenile (2.5mg/kg) and sacrificed after 24hrs. Bone marrow smear were prepared for Observation of claslogenic effect. Results show micronuclei formation in the polychroniatic erythrocyte (PCEs) in the Sodium arsenite treated mice. Claslogenecity induced by sodium arsenile was significantly reduced in mice pretreated with the extract al 100mg/kg body weight while more significant reduction was noted at 200mg/kg body weight. The result indicates a dose dependent relationship of the inhibitory activity of the extract against sodium arsenile induced clastogenecity.
  • Thumbnail Image
    Item
    Protection against 2-acetyl aminofluorene induced toxicity in mice by garlic (allium sativum), bitter kola (garcinia kola seed) and honey
    (College of Medicine, University of Ibadan, Nigeria, 2005) Odunola, O. A.; Adetutu, A.; Olorunnisola, O. S.; Ola-Davies, O. E.
    The effects of honey (Ho) and aqueous suspensions of garlic (Allium sativum) (Ga) and bitter kola (Garcina kola seed) (Bi) on the toxicities induced by 2- acetylaminofluorene (2-AAF) a model carcinogen, were investigated in mice. The animals were dosed for seven consecutive days with Ho, Ga and Bi as dietary Supplements. They were then challenged with a single intraperitoneal (i.p.) dose of 2-AAF at 50mg/kg bd. wt on the seventh day. The degree of clastogenicity was assessed using the mouse micronucleus assay while liver damage was monitored by measüring the level of gamma glutamyltransferase (y-GT) in serum and liver homogenates respectively. The results revealed that 2-AAF induced micronuclei formation in the polychromatic Crythrocytes (PCEs) of the bone marrow by about five fold in comparison to the PCEs formed in control mice. Ho, Ga, and Bi also induced micronucleus formation on their own. However, feeding of any of Ho, Ga or Bi and the administration (i.p) of 2-AAF reduced significantly, the ability of 2-AAF to induce micronuclei formation in the Order Ho>Ga>Bi. Furthermore, 2-AAF induced y-GT activity in the serum and liver homogenate by about two and a half and three folds respectively. A combination of 2-AAF and any of GaorBi or Ho significantly decreased 2-AAF-induced activity of y-GT in the order Ho>Bi>Ga (serum) and Bi>Ga=Ho (liver). These findings suggest that honey, garlic and bitter kola protect against 2-AAF-induced y-GTactivity and micronuleated PCEs formation.
  • Thumbnail Image
    Item
    Reduction of the clastogenic effect of inorganic arsenic by extracts of some dietary additives
    (Science Association of Nigeria, Sokoto, Nigeria, 2004) Odunola, O. A.; Ola-Davies, O. E.
    The clastogenic effects of aqueous' extracts of Garlic (Allium sativum.L) (Ga), African pepper (Xylopia aethiopica) (Ap), Bush pepper (Piper guincense) (Bp) and African nutmeg (Monodora myristica). (An) at 100mg/kg body weight each administered orally either alone and in combination with a single oral dose of sodium arsenite (2.5mg/kg body wciglit, 1/10th, of LD50) were investigated in mouse bone marrow cells. The results obtained show that Ga induced micronucleus formation in the polychromatic erytrocytcs (PCEs) of the bone marrow by about 12 folds followed by Bp (7 folds), Ap (4 folds) and An (1 fold) in comparison with animals exposed to distilled water only. These results indicate that Ga, Bp and Ap have mild clastogenic activity in micc. ln contrast, sodium arsenite, a known clastogen, induced micronuclcatcd PCEs formation by about 90 folds. Interestingly treatment of mice with extracts of the dietary additives Ga, Bp, Ap and An, markedly reduced the clastogenic activity of sodium arsenite in the order Ap > Ga > An > Bp. Maximum reduction of arsenite eftcct was about 60% with Bp. it may be concluded therefore from these findings that garlic, African pepper, bush pepper and African nutmeg may be useful in dietary manipulation of arsenic intoxications.