Crop Protection & Environmental Biology

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End date: 2009Subject: search.filters.subject.African yam bean

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    Effects of plant growth regulators and explant types on callus formation in African yam bean (sphenostylis stenocarpa (hochst. Ex A. rich) harms)
    (0075-5192, 2009) Akande, S. R.; Balogun, M. O.; Ogunbodede, B. A.
    This study was conducted to investigate the effect of explant types and plant growth regulator regimes on callus induction in African yam bean, with a view to developing protocols for regeneration Leaf, stem and root explants of African yam bean were cultured in media containing different types and concentrations of plant grow& regulators (PGR). The explants were observed for callus formation after three months. The main effects of variety, explant and plant growth regulator regimes and their interactions were highly significant Highest callus formation was recorded in stem explant while the root explant had the lowest. The accession, SSSWN56 brown seed) had higher percentage callus formation than ssswN7s (grey seed). No callus was formed in hormone-free medium and the media containing indole acetic acid (IAA). One- hundred-percent callus formation was recorded for both accessions in the medium containing 1.5 mg/l each of kinetin and Naphthalene acetic acid (NAA). This medium is recommended for callus induction using stem explant and it will be useful in perfecting regeneration protocols for African yarn bean.
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    Genetic variation in nutritive and anti-nutritive contents of African yam bean (sphenostylis stenocarpa)
    (Wiley InterScience, 2005) Ajibade, S. R.; Balogun, M. O.; Afolabi, O. O.; Ajomale, K. O.; Fasoyiro, S. B.
    Seeds of 20 African yam bean collections were evaluated for nutritive and antinutritive contents. Anti-nutrients were negatively correlated with protein and carbohydrate contents. Principal component analysis and the Fastclus procedure showed that collections with high anti-nutrient contents had darker seed colour.
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    Variation in seed yield and nutrient contents in African yam bean (sphenostylis stenocarpa)
    (Alexander von Humboldt Foundation, Bonn, Germany., 2008-09) Akande, S. R.; Balogun, M. O.
    Nine African yam bean accessions were evaluated for seed yield and other reproductive characters Significant differences were observed for seed yield, 100 seed weight and number of days to 50% flowering. Seed yield among the accessions ranged from 24.79 to 118.33 g per plant. Average number of seeds per pod was from 13.33 to 19.33, 100 seed weight varied between 19.69 to 28.22 g, while pod length also differed from 19.57 to 28.00 cm. The protein content of the African yam bean accessions were comparable to that of cowpea, but the concentrations of the anti-nutritional factors were significantly higher in the African yam bean accessions. Although the African yam bean accessions with low anti-nutritional factors were low yielding, they can be recombined with the high yielding accessions in developing improved varieties.