Crop Protection & Environmental Biology

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    Morphological diversity of 51 kenaf accessions in Nigeria
    (Universidad de Oriente Press, 2008) Balogun, M. O.; Raji, J. A.; Akande, S. R.
    Fifty-one accessions of Hibiscus cannabinus L. were evaluated for 14 morphological parameters in Ibadan, southwestern Nigeria. The correlation matrix of the quantitative parameters was used to perform principal components (PC) analysis to understand the relative contributions of each trait to the variation observed, while Fastclus procedure was used to cluster the accession into five groups. The most widely varied traits were earliness, number of apical branches and leaf lobes per plant with 483.3, 97.9 and 60.6% coefficients of variation respectively. Correlation between fibre yield parameters and earliness was negative and significant. The first three PCs explained 66.23% of the variation, with only number of apical branches not highly weighted by any of the PC. The five clusters were distinguished by earliness, plant height, fibre yield, stem spine density, stem girth and apical branching. The variation was seen as a manifestation of environmental response in addition to the genotypic constitution. These results will aid in parent selection during breeding programmes.
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    Variation in photo- and thermal- sensitivities among local, improved and exotic kenaf accessions in Nigeria
    (WFL Publishers, 2007) Balogun, M. O.; Raji, J. A.; Akande, S. R.; Ogunbodede, B. A.
    The response of two local, eleven improved and one exotic accessions of kenaf to daylength was determined by their increase in vegetative growth after flowering in 2004. Seven out of the fourteen accessions were planted every 4 months in 2005 to determine their sensitivity to natural variations in daylength, temperature, solar radiation and relative humidity in tenns of days to flower initiation, percentage gain in height after flowering and fibre yield. The plants took longer days to flower at longer daylength and higher temperature. Differences among accessions were significant for all traits in 2004. Days to flowering were more in December than in April and August, the number of days differing among accessions. Percentage gain in height after flowering differed with planting date only in the heat-tolerant accessions and was highest in December. Differences in fibre yield were not significant among accessions when planted in August, but differed significantly in April and December. On the average, fibre yield was highest in December and lowest in August. A grouping of the accessions based on their responses to daylength for both years of study was consistent. The implications of these findings in development of agronomically superior varieties and production of raw materials all round the year in Nigeria are discussed.
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    Effects of plant growth regulators on indirect somatic embryogenesis in kenaf (hibiscus cannabinus )
    (The Genetics Society of Nigeria, 2006) Balogun, M. O.; Akande, S. R.; Ogunbodede, B. A.
    To develop protocols for regeneration of kenaf via indirect somatic embryogenesis, hypocotyl and cotyledon explants of two genotypes were cultured at different 2,4- dichlorophenoxyacetic acid and kinetin concentrations. The highest callus yields were obtained in medium containing both 0.5mg of kinetin and 0.1mg of 2, 4-dichlorophenoxyacetic acid per litre of medium. The calli were further cultured in twelve different media containing different plant growth regulators to induce somatic embryogenesis. Friable calli were mostly produced in one genotype (Ifeken400) and non-friable in the other (Ifeken400). Among the plant growth regulator regimes, media containing 3.0mg/I of kinetin and that containing 1.5mg/l each of kinetin and NAA did not support production of globular embryos in both varieties. The friable and non- friable caili were most responsive to kinetin and NAA respectively in terms of formation of torpedo embryos. The most advanced walking stick embryos were produced only in Ifeken400 in 1.5mg/l NAA. The importance of genotype and callus type in successful regeneration of kenaf by indirect somatic embryogenesis is discussed. Research is ongoing to achieve whole plantlet regeneration.