DEPARTMENT OF ANIMAL SCIENCE

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Author: search.filters.author.Akinyemi, M. O.Subject: search.filters.subject.Polymorphism

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    BIOCHEMICAL CHARACTERISATION OF MUSCOVY AND MALLARD DUCKS IN NIGERIA
    (2014) Akinyemi, M. O.; Aina, A. J.; Ewuola, E.O.; Osaiyuwu, O.H.; Ajao, E.O.
    Protein polymorphism and alleleic variation were investigated in the blood and egg proteins of the Muscovy and Mallard duck breeds found in Southwestern Nigeria. Four proteins namely haemoglobin, transferrin, carbonic anhydrase, and albumin in the blood and two egg white proteins:ovalbumin and conalbumin were analysed .A total of 50 ducks comprising of 30 Mallard duck and 20 Muscovy ducks and 20 eggs from each breed were used for the analysis. Separation of blood and egg protein genotypes was achieved using cellulose acetate electrophoresis. Two co-dominant allele A and B controlling three genotypes AA, AB and BB were observed at haemoglobin, transferrin and carbonic anhydrase loci investigated for both breeds. A third allele C was observed at the albumin locus in the Mallard breed. . Allele A was the most predominant at the Hb, Tf and CA locus in the Muscovy breed with frequencies 0.775, 0.575, and 0.675 respectively and in the Mallard at the Tf, CA and Al locus with frequencies 0.667, 0.567 and 0.767 respectively. The frequencies of allele A and B at the conalbumin locus were 0.425, 0.575; 0.525, 0.475 in the Muscovy breed. Estimates of heterozygosity were 0.428 and 0.430 in the Muscovy and Mallard ducks respectively. Dendogram generated from the genetic distance values revealed that the two breeds are closely related. High genetic similarity was observed between the two breeds.
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    Biochemical Polymorphism in Newzealand White X Chinchilla Rabbit Crosses
    (2017) Osaiyuwu, O.H.; Akinyemi, M. O.; Akinlabi, J. O.; Fatai, R. B.
    The study evaluated diversity within New Zealand white and Chinchilla rabbit crosses using four structural protein loci: Hemoglobin (Hb), Albumin (Alb), Transferin (Tf) and Carbonic anhydrase (CA). Blood (4mls) was sampled from a total of 49 rabbits through ocular venipuncture. The samples collected were analysed using cellulose acetate electrophoresis to estimate the genetic variability within the populations. The degree of heterozygosity, deviation from Hardy Weinberg's Equilibrium (HWE), F , and F values were estimated. All four proteins IS ST loci studied were found to be polymorphic. CA and Alb produced two codominant alleles which controlled three different genotypes. While the two observed codominant alleles in Hb and Tf controlled two genotypes. The haemoglobin locus had the highest heterozygosity value (0.87) while the lowest value (0.53) was recorded at the Albumin locus. Significant deviation from Hardy-Weinberg equilibrium (P < 0.05) was observed at Hb and Tf loci while CA and Alb loci were found to conform with Hardy-Weinberg equilibrium (p > 0.05). Negative F values IS were obtained for all the studied loci indicating the deficiency of homozygotes in the population and that mates were less related in comparison to the average relationship of the population. The results obtained could serve as a reference point in the genetic improvement of the rabbit genetic resource.
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    Frequencies of the Transferrin Alleles and Genotypes in Red Sokoto Goats of Nigeria
    (2014) Akinyemi, M. O.
    The aim of this study was to determine the frequencies of Transferrin alleles and genotypes in Red Sokoto goat. The present study was conducted on 96 adult Red Sokoto goat, maintained in the Northern parts of Nigeria and transported to Bodija market in Ibadan. The polymorphism of transferrin was determined using Cellulose Acetate electrophoresis technique. It was found that the transferrin was controlled by three codominant alleles (TfA, TfB, TfC) in Red Sokoto goats. These three alleles, because of the codominant nature of inheritance, control the occurrence of four transferrin genotypes in the analyzed flock. Two (TfAA and TfBB) of these were homozygous while the other two (TfAB and TfAC) were heterozygous. It was found that the TfAB genotype (0.562) was predominant while Tf AC genotype (0.021) was least common in the analyzed flock. Frequencies of other genotypes were as: TfAA (0.292) and TfBB (0.125) for each genotype in the population. The heterozygote genotype frequency (0.583) was more than that of homozygote genotype (0.417). Considerable variations were observed in the frequencies of transferrin alleles. The frequencies of transferrin alleles were found to be TfA = 0.583, TfB = 0.406 and TfC = 0.010. Transferrin system has showed genetic equilibrium in the analyzed population (x2 value = 4.414). The value of observed heterozygosity was 0.583 with average Fis value of - 0.1795